Method for evaluating nicotine addiction degree or evaluating influence of to-be-detected medicine on nicotine addiction degree

A nicotine, level technology, applied in the field of medicine, can solve problems such as interference of experimental results, influence of experimental efficiency, influence of research conclusions, etc., to avoid experimental failure, improve experimental efficiency, and simple equipment.

Pending Publication Date: 2022-05-06
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, venous catheterization requires a higher level of proficiency for experimenters
(2) Catheters embedded under the skin of test animals will be damaged over time, and gaps will appear, which will lead to unsustainable experiments and affect the efficiency of experiments. The elimination rate of test animals is high
(3) In the intravenous self-administration experiment, the experimental animals must be placed in the control box alone
Since both rats and mice are social animals, individual experiments will inevitably cause stress, which has been proven to have a great influence on addictive behavior, so this model will have a negative impact on the experimental results. Interference, affect research conclusions
(4) The system used in the intravenous self-administration experiment is expensive, and the equipment needs to occupy a large space

Method used

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  • Method for evaluating nicotine addiction degree or evaluating influence of to-be-detected medicine on nicotine addiction degree
  • Method for evaluating nicotine addiction degree or evaluating influence of to-be-detected medicine on nicotine addiction degree

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1 Establishment of quantifiable nicotine addiction model by nicotine drinking water counting test

[0037] The invention proves the feasibility of the method by adopting the mouse nicotine drinking water counting experiment. In this experiment, there were 2 groups (experimental group and control group) of 8-week-old mice, 15 mice in each group.

[0038] Addiction phase trials were performed first. The only water source in the feeding environment for the mice in the experimental group was a mixed aqueous solution containing sodium saccharin and nicotine. The concentration of sodium saccharin in the mixed aqueous solution was 2g / L, that is, each liter of aqueous solution contained 2g sodium saccharin and the concentration of nicotine was 0.5mg / L , that is, each liter of water contains 0.5mg of nicotine, and sodium saccharin is added to cover the bitter taste of nicotine. The only water source in the feeding environment of the mice in the control group was an aqu...

Embodiment 2

[0045] Example 2 Evaluation of Quantifiable Nicotine Addiction Models Using Withdrawal Drugs

[0046] We further take technical method in the present invention to verify the effect of clinical smoking cessation drug varenicline ( figure 2 ). In this experiment, there were 2 groups of 8-week-old mice, 15 in each group. The only water source in the feeding environment of the mice in the experimental group was 0.2% sodium saccharin + 0.5 mg / kg nicotine solution; the only water source in the feeding environment of the mice in the control group was 0.2% sodium saccharin. After drinking the above water sources for three weeks, the mice were individually placed in the corresponding water bottle licking equipment filled with saccharin sodium or saccharin sodium+nicotine solution for three days, one hour a day. On the fourth day, the licking counting test was carried out, and the number of licking and sucking of each mouse was recorded in the fixed-time experiment of 4 hours per day...

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Abstract

The invention provides a method for evaluating the nicotine addiction degree or evaluating the influence of a to-be-detected medicine on the nicotine addiction degree, which comprises the following steps: S1) providing a nicotine-containing aqueous solution as the only water source of a test animal, feeding and recording the times of licking the nicotine-containing aqueous solution by the test animal, when the frequency of licking the nicotine-containing water solution by the test animal is stable, obtaining a nicotine addiction test animal model; s2) replacing a water source with a nicotine-free aqueous solution to obtain a test animal model generating nicotine withdrawal; and S3) evaluating the nicotine addiction degree by evaluating the water source licking frequency or frequency of the experimental animal generating nicotine withdrawal, or evaluating the influence of a to-be-detected medicine on the nicotine addiction degree by the water source licking frequency or frequency of the experimental animal after the to-be-detected medicine is given to the experimental animal. The preparation method of the animal model is very simple, does not need surgical procedures, and can quantitatively evaluate the addiction and withdrawal degrees of experimental animals.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for evaluating the degree of nicotine addiction or evaluating the influence of a drug to be tested on the degree of nicotine addiction Background technique [0002] Nicotine is the main addictive substance in tobacco, and it is also the main addictive ingredient in nicotine e-cigarette liquid currently on the market. At present, there is a serious lack of drugs to treat nicotine addiction at home and abroad. The research on nicotine addiction will help the development of smoking cessation drugs and help more smokers get rid of tobacco and e-cigarette dependence. Therefore, there is a great market demand and social demand for the assessment and research of nicotine addiction. However, the current commonly used method is nicotine self-administration method to evaluate and study nicotine addiction. Although this method can well quantify nicotine addiction, this method h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K31/465A01K67/027
CPCA61K49/0004A61K49/0008A61K31/465A01K67/027
Inventor 陈祖昕刘欣安莫之准
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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