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Composite microbial agent containing streptomyces and application thereof

A technology of compound microbial inoculants and streptomyces, applied in the directions of microorganisms, biocides, microorganism-based methods, etc., can solve problems such as difficult control, fruit shedding, affecting normal growth, etc., to stimulate cell division, promote rooting, improve Effects on crop yield and quality

Pending Publication Date: 2022-04-29
春华秋实科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the fruit is infected, a round black lesion will form near the fruit base, and it will harden and be slightly sunken, causing a large number of fruits to fall off; its economic value

Method used

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  • Composite microbial agent containing streptomyces and application thereof
  • Composite microbial agent containing streptomyces and application thereof
  • Composite microbial agent containing streptomyces and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the composite microbial inoculum containing streptomyces specifically comprises the following steps:

[0023] (1) Activation of bacteria

[0024] Streptomyces luteus, Streptomyces clostridium, and Streptomyces loucherii were respectively inserted into Gaoshi No. 1 medium and cultured at 25°C for 48 hours to obtain a suspension of Streptomyces;

[0025] Inoculating the P. lilacensis strain into the PDA medium and culturing it at 25°C for 48 hours to obtain the P. lilacinum suspension;

[0026] Insert the Trichoderma harzianum species into the PDA medium and culture at 25°C for 72h to obtain the Trichoderma harzianum suspension;

[0027] (2) Bacterial strain expansion culture: each bacterial suspension in step (1) is respectively connected to a fermenter at 28°C for 8 days of fermentation and culture, and then dried and pulverized under reduced pressure to obtain each bacterial agent; the viable bacteria in each bacterial agent The number is no...

Embodiment 2

[0030] The preparation method of the composite microbial inoculum containing streptomyces specifically comprises the following steps:

[0031] (1) Activation of bacteria

[0032] Streptomyces luteus, Streptomyces clostridium, and Streptomyces loucherii were respectively inserted into Gaoshi No. 1 medium and cultured at 28°C for 36 hours to obtain a suspension of Streptomyces;

[0033] Inoculate the P. lilacensis strain into the PDA medium and culture it at 28° C. for 36 hours to obtain the P. lilacinum suspension;

[0034] Insert Trichoderma harzianum species into PDA medium and culture at 28°C for 60h to obtain Trichoderma harzianum suspension;

[0035] (2) Bacterial strain expansion cultivation: respectively put each bacterial suspension in step (1) into a fermenter at 28°C for fermentation and cultivation for 8 days under reduced pressure, dry and pulverize to obtain each bacterial powder; the number of viable bacteria in each bacterial powder Not less than 20 million pie...

Embodiment 3

[0038] The preparation method of the composite microbial inoculum containing streptomyces specifically comprises the following steps:

[0039] (1) Activation of bacteria

[0040] Streptomyces luteus, Streptomyces clostridium, and Streptomyces loucherii were respectively inserted into Gaoshi No. 1 medium and cultured at 28°C for 36 hours to obtain a suspension of Streptomyces;

[0041] Inoculate the P. lilacensis strain into the PDA medium and culture it at 28° C. for 36 hours to obtain the P. lilacinum suspension;

[0042] Insert Trichoderma harzianum species into PDA medium and culture at 28°C for 60h to obtain Trichoderma harzianum suspension;

[0043] (2) Expansion of bacterial strains: put each bacterial suspension in step (1) into a fermenter at 30°C for 6 days of fermentation and cultivation under reduced pressure, dry and pulverize to obtain each bacterial agent; the number of viable bacteria in each bacterial agent is different Less than 20 million pieces / g;

[0044...

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PUM

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Abstract

The invention discloses a composite microbial agent containing streptomycete, which is characterized in that the composite microbial agent comprises streptomycete, purpureocillium lilacinum and trichoderma harzianum; the streptomyces comprises streptomyces microflavus, streptomyces dense and streptomyces rochei; the weight ratio of the streptomyces to the purpureocillium lilacinum to the trichoderma harzianum is (4-6): (1-3): 1; according to the compound microbial agent prepared by the invention, various strains are reasonably proportioned and have a synergistic effect, so that gray mold and leaf mold of crops can be efficiently prevented and treated; meanwhile, crop growth can be stimulated, and crop yield and quality are improved.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a composite microbial preparation containing streptomyces and an application thereof. Background technique [0002] With the rapid development of my country's agricultural economy, a large number of chemical fungicides have been applied to the prevention and control of plant diseases and insect pests; chemical fungicides not only prevent and control diseases, but also kill beneficial organisms, hindering the balanced development of the ecological environment; at the same time, chemical fungicides The long-term use of pesticides has caused the resistance of pathogenic bacteria that induce plant diseases, forming a vicious circle; the use of biological control fungicides as biofungicides to control plant pathogens to reduce the impact of chemical pesticides on the ecological environment has attracted widespread attention. . [0003] Botrytis cinerea and leaf mold oc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/38A01N63/30A01N63/28A01P3/00C12R1/465C12R1/645C12R1/885
CPCC12N1/20C12N1/14A01N63/38A01N63/30A01N63/28
Inventor 祝春华李献伟焦怀志余冬冬张兰平
Owner 春华秋实科技集团有限公司
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