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Method for reducing and degrading enrofloxacin by using biological cathode co-metabolism system

A technology of enrofloxacin and biocathode, which is applied in biological treatment devices, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems that do not involve ENR removal performance and dominant microbial communities, and achieve rich quantity and variety , reduce toxicity, promote the effect of reductive degradation

Active Publication Date: 2022-04-29
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN107601677A discloses a method for degrading trimethoprim using microbial fuel cells. Chinese patent CN112473360A discloses a method for anaerobically treating chlorobenzene waste gas in a microbial electrolytic cell. The prior art does not involve ENR in a bioelectrochemical system Biocathode removal performance and dominant microbial community, therefore, it is of great significance to establish a novel, fast and efficient ENR treatment method

Method used

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  • Method for reducing and degrading enrofloxacin by using biological cathode co-metabolism system
  • Method for reducing and degrading enrofloxacin by using biological cathode co-metabolism system
  • Method for reducing and degrading enrofloxacin by using biological cathode co-metabolism system

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Effect test

Embodiment 1

[0026] A method for reducing and degrading enrofloxacin by using a biocathode co-metabolism system, the anode chamber and the cathode chamber of the MFC reactor are respectively injected with anolyte and catholyte:

[0027] Anolyte, every 1L of water includes the following components by weight: 1.28g sodium acetate, 0.13g KCl, 0.31g NH 4 Cl, 11.55g Na 2 HPO 4 -12H 2 O, 2.77g NaH 2 PO 4 -2H 2 O, 1mL Wolfe vitamin trace element solution and 1mL Wolfe mineral element solution;

[0028] Catholyte, every 1L of water includes the following components by weight: 0.025g enrofloxacin, 1.28g sodium acetate, 0.13gKCl, 0.31g NH 4 Cl, 11.55g Na 2 HPO 4 -12H 2 O, 2.77g NaH 2 PO 4 -2H 2 O, 1mL Wolfe vitamin trace element solution and 1mL Wolfe mineral element solution;

[0029] Among them, Wolfe vitamin trace element solution contains the following components by weight per 1L of water: 2mg vitamin H, 2mg folic acid, 10mg pyridoxine hydrochloride, 5mg thiamine, 5mg riboflavin, 5m...

Embodiment 2

[0032] A method for reducing and degrading enrofloxacin by using a biocathode co-metabolism system, the anode chamber and the cathode chamber of the MFC reactor are respectively injected with anolyte and catholyte:

[0033] Anolyte, every 1L of water includes the following components by weight: 1.00g sodium acetate, 0.13g KCl, 0.31g NH 4 Cl, 11.55g Na 2 HPO 4 -12H 2 O, 2.77g NaH 2 PO 4 -2H 2 O, 1mL Wolfe vitamin trace element solution and 1mL Wolfe mineral element solution;

[0034] Catholyte, every 1L of water includes the following components by weight: 0.02g enrofloxacin, 1.00g sodium acetate, 0.13gKCl, 0.31g NH 4 Cl, 11.55g Na 2 HPO 4 -12H 2 O, 2.77g NaH 2 PO 4 -2H 2 O, 1mL Wolfe vitamin trace element solution and 1mL Wolfe mineral element solution;

[0035] Among them, Wolfe vitamin trace element solution contains the following components by weight per 1L of water: 2mg vitamin H, 2mg folic acid, 10mg pyridoxine hydrochloride, 5mg thiamine, 5mg riboflavin, 5mg...

Embodiment 3

[0038] A method for reducing and degrading enrofloxacin by using a biocathode co-metabolism system, the anode chamber and the cathode chamber of the MFC reactor are respectively injected with anolyte and catholyte:

[0039] Anolyte, every 1L of water includes the following components by weight: 1.68g sodium acetate, 0.13g KCl, 0.31g NH 4 Cl, 11.55g Na 2 HPO 4 -12H 2 O, 2.77g NaH 2 PO 4 -2H 2 O, 1mL Wolfe vitamin trace element solution and 1mL Wolfe mineral element solution;

[0040] Catholyte, each 1L of water includes the following components by weight: 0.03g enrofloxacin, 1.80g sodium acetate, 0.13gKCl, 0.31g NH 4 Cl, 11.55g Na 2 HPO 4 -12H 2 O, 2.77g NaH 2 PO 4 -2H 2 O, 1mL Wolfe vitamin trace element solution and 1mL Wolfe mineral element solution;

[0041] Among them, Wolfe vitamin trace element solution contains the following components by weight per 1L of water: 2mg vitamin H, 2mg folic acid, 10mg pyridoxine hydrochloride, 5mg thiamine, 5mg riboflavin, 5mg ...

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Abstract

The invention provides a method for reductive degradation of enrofloxacin by using a biological cathode co-metabolism system. The method comprises the following steps: constructing an MFC reactor, taking a carbon brush as a cathode and an anode, respectively injecting anolyte and catholyte with a specific ratio into an anode chamber and a cathode chamber, inoculating anaerobic sludge into the two chambers, and starting and domesticating the MFC reactor. The adopted bioelectrochemical system not only improves the reduction and degradation efficiency of enrofloxacin, but also can enrich microbial communities of anode and cathode biological membranes, increases the number of electrogenesis flora and degradation flora, reduces the toxicity of enrofloxacin metabolites, does not need to use an expensive catalyst, is low in cost, and is suitable for industrial production. The method is an effective and environment-friendly method and has important application prospects.

Description

technical field [0001] The invention relates to the field of microbial electrochemistry, belongs to the technical field of biocathode degradation and detoxification of enrofloxacin, and particularly relates to a method for reducing and degrading enrofloxacin by using a biocathode co-metabolism system. Background technique [0002] Worldwide use of antibiotics increased by 36% between 2000 and 2010. It is reported that the usage of antibiotics in the world has increased by 40% in 15 years, resulting in a large amount of residues inevitably entering the water body and soil environment, especially in the water body environment, where the content is extremely widely distributed. At present, hundreds of antibiotic residues have been found in rivers and lakes, groundwater, drinking water, seawater and sewage treatment plants. Residual antibiotics produce resistant bacteria (ARB) and resistant genes (ARG) through gene mutation or horizontal transfer, which will make the efficacy o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/00C02F3/34C02F101/30
CPCC02F3/005C02F3/34C02F2101/30C02F2203/004C02F2203/006C02F2305/06
Inventor 王德欣薛小芳
Owner HAINAN UNIVERSITY
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