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Detection method of trigonelline and application of trigonelline in honey identification

A technology of trigonelline and a detection method, applied in the field of chemical detection, can solve problems such as damage to consumers' rights and interests, and achieve the effects of accurate separation and rapid analysis

Pending Publication Date: 2022-04-22
秦皇岛海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to pursue high profits, unscrupulous companies often use low-priced syrup to mix or pretend to be rapeseed honey, or use rapeseed honey to mix or pretend to be high-priced honey, thereby harming the rights and interests of consumers
At present, my country has not yet formulated the identification standards for rapeseed honey. How to effectively evaluate and identify rapeseed honey has become one of the problems that need to be solved urgently in the current bee product industry.

Method used

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  • Detection method of trigonelline and application of trigonelline in honey identification
  • Detection method of trigonelline and application of trigonelline in honey identification
  • Detection method of trigonelline and application of trigonelline in honey identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] Example 1 Method optimization, detection limit and quantification limit for the determination of trigonelline content in honey

[0132] After testing, the honey sample can be directly detected by LC-MS / MS after dissolving in water and distilled to volume with acetonitrile water. Therefore, the experimental conditions of LC-MS / MS were mainly selected and optimized.

[0133] According to the properties of trigonelline, the most commonly used C18 chromatographic column and HILIC chromatographic column were selected to compare the separation effect, see the attached figure 1 . The results showed that the peak shape, sensitivity and retention performance of trigonelline were better than those of C18 chromatographic column when using HILIC chromatographic column, so HILIC chromatographic column was adopted. See the attached MRM chromatogram for the detection of trigonelline figure 2 .

[0134] With 3 times of signal-to-noise ratio corresponding sample concentration as met...

Embodiment 2

[0135] Example 2, the establishment of trigonelline as a characteristic marker of rapeseed honey

[0136] Sample source: The honey samples used in this study are all natural mature honey collected in the flowering and nectar-flowing season of each nectar source plant. All samples were purchased directly from beekeepers and stored at 4°C before testing.

[0137] The analysis method established by the present invention was used to detect the collected honey samples of 21 different nectar-source plants, and a total of 543 batches of samples were detected. Among them: 168 batches of rape honey, 52 batches of acacia honey, 52 batches of Vitex honey, 52 batches of linden honey, 52 batches of lychee honey, 52 batches of jujube honey, 15 batches of honey, 10 batches of buckwheat honey, 10 batches of sunflower honey, and sweet potato 10 batches of honey, 10 batches of milk vetch honey, 10 batches of rubber honey, 10 batches of longan honey, 5 batches of gallnut honey, 5 batches of schi...

Embodiment 3

[0143] Embodiment 3, identification of rapeseed honey components in commercially available honey

[0144] Source of samples: buy 20 batches of rapeseed honey and 10 batches of acacia honey from supermarkets or the Internet, use the method of the present invention to detect trigonelline in the samples, and determine whether rapeseed honey is contained therein.

[0145] the result shows:

[0146] (1) Among the 20 batches of rapeseed honey, 14 batches of honey have a trigonelline content between 34 and 88 mg / kg, meeting the requirements of the content range of rapeseed honey trigonelline in Example 2, and are determined to be real rapeseed honey; the remaining 6 batches of rapeseed honey The contents of 3 batches of honey were 2.2mg / kg, 6.5mg / kg and 11.0mg / kg respectively, and 3 batches of rapeseed honey were not detected. These samples were determined to be impure rapeseed honey or adulterated rapeseed honey.

[0147] (2) Among the 10 batches of acacia honey, 3 batches of trigo...

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Abstract

The invention relates to a detection method of trigonelline and application of the trigonelline in honey identification, and the detection method of the trigonelline comprises the following steps: (1) dissolving a sample with an aqueous solution, and diluting with acetonitrile; (2) detecting the trigonelline in the honey sample by adopting a liquid chromatography-tandem mass spectrometer; furthermore, the rape honey can be effectively identified according to the content of the trigonelline in the honey.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a detection method of trigonelline and its application in honey identification. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, mix with their own secretions, and fully brew. Rapeseed honey is honey brewed by bees from the pollen and nectar of rapeseed flowers. It has the aroma of rapeseed flowers. Anemia and regulation of immune function have a certain effect. Rapeseed honey is mainly produced in all parts of the country, and it is the single flower honey variety with the largest annual output and export volume in my country. Rapeseed flowers are widely planted in my country and the cost is low, and the output of rapeseed honey products is large and stable, resulting in a lower price of rapeseed honey than other single-flower honeys such as acacia, Vitex, and linden. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/8675G01N2030/324
Inventor 崔宗岩黄学者贾英杰母健贾光群李亮曹彦忠张进杰
Owner 秦皇岛海关技术中心
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