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Kit for expanding quantitative detection range and detection method

A quantitative detection and detection method technology, applied in the field of in vitro diagnostics and immunological detection, can solve the problems of narrow quantitative detection range of macromolecular proteins, achieve the effect of delaying HOOK effect, simple calculation method, and improving precision

Active Publication Date: 2022-04-12
天津中新科炬生物制药股份有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies in the prior art and provide a kit and a detection method that expands the quantitative detection range, so as to overcome the problem that the existing macromolecular protein quantitative detection range is narrow

Method used

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  • Kit for expanding quantitative detection range and detection method
  • Kit for expanding quantitative detection range and detection method
  • Kit for expanding quantitative detection range and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: detect the HCG antigen concentration in the sample with the test kit among the present invention

[0044] 1. Materials and instruments

[0045] 13 groups of HCG antigen standard samples with the sample concentration range of 10-200000mIU / mL and sample numbers N1-N13 (diluted from HCG antigen, see Table 1 for specific concentrations).

[0046] F10Pro fluorescent immunoassay analyzer, timer (such as stopwatch) and pipette produced by Tianjin Zhongxin Keju Biopharmaceutical Co., Ltd.

[0047] The microfluidic chip for detecting the concentration of HCG antigen prepared according to the improved technical scheme of the present invention (the chip includes a cover sheet and a bottom sheet that are fastened to each other, and the cover sheet is respectively provided with a sample injection hole, a microchannel and a waste sheet along the flow direction of the sample. Liquid tank, the area where the bottom plate is facing the microchannel is provided with a labe...

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Abstract

The invention provides a kit for expanding a quantitative detection range and a detection method, the kit comprises a carrier, the carrier is provided with a labeled site, a T line and a C line, the labeled site contains a labeled antibody which is subjected to a specific reaction with a sample antigen and is labeled by signal particles, and the T line is connected with the C line. A capture antibody which is subjected to double-antibody sandwich reaction with a sample antigen and a labeled antibody is fixed on the T line, the capture antibody is formed by mixing two antibodies with different combining capacities with the sample antigen, and an antigen-antibody conjugate which is subjected to double-antibody sandwich reaction with the labeled antibody is fixed on the C line. A capture antibody on the T line is formed by mixing two antibodies with different binding capacities, generation of the HOOK effect is delayed, a directional coating method is adopted on the C line, for a to-be-detected sample with higher concentration, an antigen-antibody conjugate can show a more remarkable competition gradient when the antigen and a sample antigen compete for a labeled antibody, the descending range of the C line is widened, and the detection accuracy is improved. And the quantitative detection range of macromolecular proteins is expanded.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis and immune detection, and in particular relates to a kit and a detection method for expanding the range of quantitative detection. Background technique [0002] Immunoassay is an analytical method that uses antigen-antibody specific binding reactions to detect the presence or concentration of drugs, hormones, proteins, microorganisms and other substances in complex samples. According to the different reaction mechanisms, immunoassays can be divided into competitive and non-competitive methods. Among them, the non-competitive method is to fully react the antigen to be tested with enough labeled antibody to form an antigen-labeled antibody conjugate, and the signal intensity generated is proportional to the amount of antigen. The competition method is to combine the antigen to be tested with quantitatively labeled antigen (direct competition) or immobilized antigen (indirect competition)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/577G01N33/558B01L3/00
Inventor 李昀地王俊水李慧许人茂周洪锐魏华英刘钟泉赵娜张粲肖福磊秦月李轩贺敬文刘陈针玉钱龙李娜孟佳李洲
Owner 天津中新科炬生物制药股份有限公司
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