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Preparation method of trichoderma wettable powder

A wettable powder, Trichoderma technology, applied in the field of microbial fermentation, can solve the problems of long growth cycle, poor mycelium resistance, low survival rate, etc.

Inactive Publication Date: 2022-04-12
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mycelium has poor stress resistance and low survival rate. Although chlamydospores have strong storage capacity, the culture conditions are harsh and the growth cycle is long.

Method used

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  • Preparation method of trichoderma wettable powder
  • Preparation method of trichoderma wettable powder
  • Preparation method of trichoderma wettable powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of Trichoderma wettable powder, comprising the following steps:

[0033] S1. Preparation of conidia suspension: transfer the strain T.brev T069 to PDA medium for activation culture, cultivate a large number of green spores at a constant temperature of 20°C for 7 days, wash the spores with sterile water, and dilute to 0.8×10 8 The seed solution of CFU / mL is ready for use;

[0034] S2, preparation of fermentation substrate: collect the cassava girdling skin, cut it into small pieces, dry, pulverize, pass through 10 mesh cassava skin dregs, and mix evenly, weigh cassava skin dregs, dissolve in water, and pack into tissue culture Sterilize in the bottle to obtain the fermentation substrate;

[0035] S3. Preparation of conidia powder: add the T.brev T069 spore suspension into the above-mentioned tissue culture bottle according to the inoculation amount of 0.3% by volume of the fermentation substrate, shake it well, and culture it in a constant temperatu...

Embodiment 2

[0038] A preparation method of Trichoderma wettable powder, comprising the following steps:

[0039] S1. Preparation of conidia suspension: transfer the bacterial strain T.brev T069 to PDA medium for activation culture, cultivate a large number of green spores at 30°C for 11 days, wash the spores with sterile water, and dilute to 1.2×10 8 The seed solution of CFU / mL is ready for use;

[0040] S2. Preparation of fermentation substrate: Collect cassava girdling skins, cut them into small pieces, dry them, pulverize them, pass through 10-100 meshes of cassava skin residues, and mix them evenly, weigh cassava skin residues, dissolve them in water, and put Sterilize in the tissue culture bottle to obtain the fermentation substrate;

[0041] S3. Preparation of conidia powder: add the T.brev T069 spore suspension into the above-mentioned tissue culture bottle according to the inoculation amount of 1.2% by volume of the fermentation substrate, and shake it evenly, and culture it in a c...

Embodiment 3

[0044] A preparation method of Trichoderma wettable powder, comprising the following steps:

[0045] S1. Preparation of conidia suspension: transfer bacterial strain T.brev T069 to PDA medium for activation culture, cultivate a large number of green spores at 28°C for 9 days, wash the spores with sterile water, and dilute to 1.0×10 8 The seed solution of CFU / mL is ready for use;

[0046] S2. Preparation of fermentation substrate: Collect cassava girdling skins, cut them into small pieces, dry them, pulverize them, pass through 10-100 meshes of cassava skin residues, and mix them evenly, weigh cassava skin residues, dissolve them in water, and put Sterilize in the tissue culture bottle to obtain the fermentation substrate;

[0047] S3. Preparation of conidia powder: add the T.brev T069 spore suspension into the above-mentioned tissue culture bottle according to the inoculation amount of 1% by volume of the fermentation substrate, shake it well, and culture it in a constant tem...

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Abstract

The invention provides a preparation method of trichoderma wettable powder, which comprises the following steps: S1, preparation of conidium suspension, S2, preparation of fermentation substrate, S3, preparation of conidium powder, and S4, preparation of the wettable powder, the composition proportion of the wettable powder is determined to be 20% of conidium powder, 1% of AB-3, 5% of dispersant NNO, 1% of ascorbic acid, and the balance of water. According to the present invention, the wetting time, the mass suspension rate and other aspects of the strain meet the related standards of the pesticide wettable powder, the important significance is provided for the development and the factory production of the T.brev T069 product, the composition of the strain wettable powder is determined, and the foundation is laid for the production development, the field application and the pre-evaluation of the biocontrol T.brev T069.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of Trichoderma wettable powder. Background technique [0002] In my country's traditional agriculture, continuous cropping is very common, which will lead to serious continuous cropping obstacles, namely, the decrease of soil nutrients, the accumulation of pathogenic bacteria, the incidence of disease and the severity of diseases increase year by year, seriously affecting the yield and quality of crops. In order to better control diseases, people often use a large amount of pesticides and chemical fertilizers, but the effect is not obvious. Moreover, the long-term and large-scale use of chemical agents will seriously affect the ecological environment and human health. [1] , and it is easy for pathogenic bacteria to develop drug resistance, which increases the difficulty of disease control. Biological control is an environmentally friendly dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/38A01N25/14A01N25/22A01P3/00C12N3/00C12N1/14C12R1/885
Inventor 刘铜张成
Owner HAINAN UNIVERSITY
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