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A blood substitute and its preparation method

A technology of blood substitute and base liquid, applied in the field of biochemistry, can solve the problems of difficult degree of hemoglobin polymerization reaction, difficult repetition of molecular cross-linking reaction, uncertainty of active ingredient components, etc., so as to improve the yield of effective products, Reduced production costs and no pathogen contamination

Active Publication Date: 2009-07-01
西安血氧生物技术有限公司
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Problems solved by technology

Therefore, molecular cross-linking reactions induced by the glutaraldehyde method are often not easily reproducible
In addition, the selectivity of the glutaraldehyde method to the target molecule is poor, and the composition of the active ingredient is uncertain, which makes it difficult to achieve the polymerization reaction of hemoglobin by controlling the concentration of reactants, reaction temperature and time when the glutaraldehyde method polymerizes hemoglobin. effectively control the extent of

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Embodiment Construction

[0026] The implementation requirements and effects of the technical solution of the present invention will be described in detail below in conjunction with examples.

[0027] 1. Pretreatment of raw materials

[0028] The raw material of the present invention-hemoglobin source can adopt the hemoglobin that obtains in the blood of various livestock poultry and people, or the recombinant hemoglobin of gene expression, remove pathogen with ultrafiltration method (filter membrane pore size 50KD), purify with chromatographic method; The physical and chemical indicators of hemoglobin are required as follows: hemoglobin purity>99.9% (as determined by HPLC method), of which oxyhemoglobin is 90-100%, carboxyhemoglobin is 0-2%, methemoglobin is 0-1%, pH7.3-7.5, endotoxin <0.05EU / ml, phospholipids <lnanomole / ml, hemoglobin affinity 28-30mmHg. Finally, the hemoglobin is dissolved in sodium phosphate buffer or Tris-HCl buffer containing 0-100 mM NaCl, pH 7.2-8.0, and the hemoglobin content...

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Abstract

A blood substitute and its preparation method, the present invention is characterized by using physiological buffer as the base liquid and hemoglobin polymer, the molecular weight of the hemoglobin polymer is between 64-550KD; the key of its preparation method is to use polymerization reaction Realization; the present invention successfully solves the technical problems in the glutaraldehyde polymerization reaction, and can effectively control the molecular weight and reaction degree of the generated polymer. The application of its products is not limited by individual blood type, non-toxic protozoa pollution, and can be stored at room temperature for more than one year; it not only has the effect of expanding blood volume, but also has the same ability to carry oxygen and CO as red blood cells 2 It is suitable for the treatment of anemia and various degrees of medical and surgical blood loss, wartime and large-scale accidental injuries; the substitute can be degraded in the body, and there is no long-term retention problem in the body, and the small molecular weight polymer also has blood transfusion It does not have the function of anti-septic shock.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a hemoglobin polymer (blood substitute) and a preparation method thereof. Background technique [0002] Blood transfusion is one of the important means of clinical treatment. It is not only a common method for the treatment of severe anemia in surgery and medicine, but also the most effective treatment for severe blood loss (such as severe bleeding trauma and other surgical emergencies) during wartime. The widespread clinical application of blood transfusion has sharply increased the demand for therapeutic blood. In fact, the clinical blood demand is often significantly greater than the supply, which leads to a serious shortage of blood sources, which is a frequent problem. In addition, infectious diseases, such as hepatitis and AIDS, which seriously endanger human health, pollute the blood source, which also increases the pressure on the supply of healthy bloo...

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Application Information

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IPC IPC(8): A61K38/42A61K35/14
Inventor 但宁陈超吴保平
Owner 西安血氧生物技术有限公司
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