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SSR (Simple Sequence Repeat) fingerprint spectrum identification method and construction method of golden yellow stropharia rugoso-annulata strain

The technology of a large ball of mushrooms and a fingerprint spectrum is applied in the field of SSR fingerprint identification of the golden yellow ball of ball mushroom strains, which can solve the problems affecting the rapid development of the large ball of ball mushroom industry, the economy of production enterprises with synonyms, and the mixed together, etc. problems, to achieve good application prospects, short detection time, and good repeatability.

Pending Publication Date: 2022-04-08
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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Problems solved by technology

After obtaining the excellent strains that have been verified by experiments, it is necessary to accurately define their varieties, so as to facilitate the distinction of different strains, so as to avoid the mixing of the selected excellent strains with other same varieties in the process of production, operation and market circulation. The phenomenon of homonym and synonym and the economic losses brought to the production enterprises affect the rapid development of the Stropharia stropharia industry

Method used

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  • SSR (Simple Sequence Repeat) fingerprint spectrum identification method and construction method of golden yellow stropharia rugoso-annulata strain
  • SSR (Simple Sequence Repeat) fingerprint spectrum identification method and construction method of golden yellow stropharia rugoso-annulata strain
  • SSR (Simple Sequence Repeat) fingerprint spectrum identification method and construction method of golden yellow stropharia rugoso-annulata strain

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[0052] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0053] see Figure 1-9 Shown, a kind of construction method of Stropharia aureus strain comprises the following steps:

[0054] S1, mycelia culture: transfer the Stropharia aureus strain to the potato dextrose agar solid medium (PDA), and collect the mycelium after cultivating for 15d at 25°C;

[0055] S2 Genomic DNA extraction: TSINGKE Plant DNA Extraction Kit (TSP101-200) was used to extract the genomic DNA of the above hyphae, and the concentration and quality ...

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Abstract

The invention belongs to the technical field of strain quality identification, and particularly relates to a fingerprint identification method and a construction method of a golden yellow stropharia rugoso-annulata strain, and is characterized in that a fingerprint constructed by six pairs of SSR (Simple Sequence Repeat) markers consists of the following primers: DQGGSSR007, DQGGSSR031, DQGGSSR034, DQGGSSR037, DQGGSSR065 and DQGGSSR088. The construction method comprises the following steps: S1, mycelium culture: transferring a golden yellow stropharia rugoso-annulata strain to a potato dextrose agar solid medium (PDA), culturing at 25 DEG C for 15 days, and then collecting mycelia; and S2, extraction of genome DNA: extracting the genome DNA of the hypha by using a TSINGKE plant DNA extraction kit (TSP101-200), and detecting the concentration and quality of the total genome DNA by using an ultraviolet spectrophotometric method. Compared with conventional morphological detection, antagonism tests and fruiting tests, the method disclosed by the invention has the advantages of short detection time, high accuracy and good repeatability. The operation time required by detection is within 24 hours, while the time required by a conventional antagonism test is at least two weeks, and the time required by a fruiting test is at least three months.

Description

technical field [0001] The invention belongs to the technical field of strain quality identification, and specifically relates to an SSR fingerprint identification method and a construction method of a Stropharia aureus strain. Background technique [0002] Stropharia rugosoannulata Farl.ex Murrill, also known as Stropharia rugosoannulata Farl.ex Murrill, also known as Stropharia rugosoannulata, wine red Stropharia, etc., and its trade name is red matsutake. Numerous studies have shown that the fruiting bodies of Stropharia stropharcoides are rich in protein, vitamins, minerals, polysaccharides and other nutrients. It is one of the famous edible fungi artificially cultivated in Europe and the United States, and it is also recommended by the Food and Agriculture Organization of the United Nations (FAO) to developing countries. One of the cultivated edible fungi. Secondly, Stropharia polysaccharide has the effects of preventing coronary heart disease, aiding digestion, reliev...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12Q1/686G16B30/00
Inventor 刘绍雄孙达锋华蓉李雪松张俊波罗孝坤马明尚陆娥
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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