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Biological preparation for improving fermentation quality of mixed silage and preparation method thereof

A technology of biological preparations and mixed silage, applied in the preservation method of animal feed raw materials, bacteria used in food preparation, animal feed, etc., can solve the problems of low digestion and utilization rate, poor palatability, etc. The effect of reducing ammonia content and enhancing palatability

Active Publication Date: 2022-03-29
SHANDONG JIANYUAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the existence of anti-nutritional factors, untreated woody feed directly fed to livestock has poor palatability and low digestibility, so silage is required.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of mulberry leaves

[0038]a. Pulling 100g mulberry leaves over 20 mesh sieves, mixed with 750 ml of water to obtain mulberry liquid, add 4 g of cellulase and 4 g of pectin enzyme at 50 ° C for 18 h after taking 200g of mulberry, and filtered Enzymatic solution;

[0039] b. Concentrates the enzymatic hydrolyzide at 65 ° C, the vacuum is 80 kPa, and the immersion paste is obtained. The dip polyamide column (a diameter ratio 1: 10) was subjected to elution at a volume ratio of 4: 1 ethanol-ethyl acetate for eluent, and the eluent was 3 times the volume of the column. The eluate was collected, and the mulberry leaves were obtained after spraying.

Embodiment 2

[0040] Example 2: Preparation of Jernesami Extract

[0041] a. Crush the tuber tuber and dry to the water content of 8% by weight to obtain Jerusalemly particles;

[0042] b. Add a 1.5L volume fraction of 80% ethanol to 100 g of Jerusalemosae particles, heating to 70 ° C for reflux extraction of 40 min, and then filtered, then filtered, concentrated, and the spray is dried. Justai extract.

Embodiment 3

[0044] (1) Put the Bacillus CICC 20293 and the Plant Lactobacillus CICC 20765 on the MRS solid beveled, Bacillus, Dalchobacillus, and the plant Lactobacillus cultured at 35 ° C, and activated 2 times Take a single bacteria into the MRS liquid medium, shake the flask overnight (100 rpm / min) to obtain the bacilli seed fluid of Burda Lactobacillus seed fluid and plant lactobacterium seed fluid, the concentration of the Bacillus Lactobacillus seed fluid and plant lactobacterium seed fluid concentration Adjust to 1.0 × 10 9 CFU / ml.

[0045] (2) Take 1 L MRS liquid medium, add 80 g of the mulberry leaf extract prepared, 150 g of the jergsum extract prepared, mixed uniformly to ferment medium, and the fermentation medium is divided into 2 parts. The 50 ml of Burda Lactobacillus seed fluid and 50 ml of plant lactofacarium seed fluid were inoculated with fermentation medium, 36 hours of fermentation of 36 h, and filtered, respectively, the bull lactobacillus fermentation liquid and pla...

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Abstract

The invention discloses a biological preparation for improving the fermentation quality of mixed silage and a preparation method thereof, and belongs to the field of silage additives. The method comprises the following steps: (1) respectively culturing lactobacillus buchneri and lactobacillus plantarum to obtain a lactobacillus buchneri seed solution and a lactobacillus plantarum seed solution; and (2) respectively inoculating the lactobacillus buchneri seed solution and the lactobacillus plantarum seed solution into a fermentation culture medium containing a mulberry leaf extract and a jerusalem artichoke extract for culturing, filtering to obtain a lactobacillus buchneri fermentation solution and a lactobacillus plantarum fermentation solution, and mixing to obtain the high-activity biological preparation. The mulberry leaf extract and the jerusalem artichoke tuber extract are added into a culture medium, and a fermentation strain is cultured to obtain the high-activity biological preparation. After use, the fermentation speed is high, lactic acid can be quickly generated, growth of putrefying bacteria is effectively inhibited, and forage is prevented from mildewing and rotting; the palatability of the silage is enhanced, and the contents of crude proteins, organic acids, vitamins and amino acids are all increased.

Description

Technical field [0001] The present invention relates to the field of silage additives, and more particularly to a biological preparation and preparation method thereof in improving the fermentation quality of mixed silage. Background technique [0002] At present, there are mainly three of the different silage of silage at home and abroad. First, ammonia treatment, adding nitrogen-containing chemical fertilizers such as carbon ammonium, urea and other chemical fertilizers to straw, inhibiting the activities of microorganisms to achieve preservation, this method The advantage is that it is possible to maintain fresh green silament, and it can improve the non-protein nitrogen nitrogen, but the disadvantage is that the pH is high. It is easy to infect the rotation of macaronia after exposure to air, and excessive non-protein nitrogen is in the green fermentation process. Teminaire is prone to melamine. Causes livestock after eating, the renal burden has affected fertility, even poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/12A23K30/18
CPCA23K10/30A23K10/12A23K30/18A23V2400/11A23V2400/169
Inventor 王诚董桂红何荣彦李燕赵福平王玲巩倩邢烛宗倩刘雨董晓桐
Owner SHANDONG JIANYUAN BIOTECHNOLOGY CO LTD
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