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Gelatin composite embolism microsphere containing nano calcium carbonate, preparation method of gelatin composite embolism microsphere and drug-loaded embolism microsphere

A nano-calcium carbonate and gelatin technology, applied in medical science, pharmaceutical formula, drug delivery, etc., can solve the problems of inability to regulate the micro-acid environment of tumors, prone to drug burst release, and eliminate lactic acid accumulation, etc., to achieve good biological safety and degradability, low cost, enhanced therapeutic efficacy

Pending Publication Date: 2022-03-25
LISHUI CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional gelatin microspheres often have problems such as low drug loading and drug burst release, and it is also difficult to eliminate the accumulation of lactic acid in the tumor during embolization therapy, and cannot regulate the microacid environment of the tumor

Method used

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  • Gelatin composite embolism microsphere containing nano calcium carbonate, preparation method of gelatin composite embolism microsphere and drug-loaded embolism microsphere
  • Gelatin composite embolism microsphere containing nano calcium carbonate, preparation method of gelatin composite embolism microsphere and drug-loaded embolism microsphere
  • Gelatin composite embolism microsphere containing nano calcium carbonate, preparation method of gelatin composite embolism microsphere and drug-loaded embolism microsphere

Examples

Experimental program
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Effect test

Embodiment 1

[0059] A gelatin composite embolic microsphere containing nano-calcium carbonate, the components in the microsphere include: 10-50% nano-calcium carbonate and 50-90% gelatin;

[0060] It is prepared by the following method:

[0061] 1) Preparation of nano-calcium carbonate suspension:

[0062] 1-1) Weigh 20mM polyacrylic acid (molecular weight 2000Da) and 20mM calcium chloride and dissolve in 200mL deionized water, stir at room temperature for 1h to obtain a mixed solution;

[0063] 1-2) Dissolve 20mM sodium carbonate in 200mL of deionized water, then quickly add it to the mixed solution obtained in step 1), and stir at room temperature for 1h;

[0064] 1-3) After the reaction, centrifuge at 12000rpm for 15min to get the precipitate, wash the resulting precipitate with deionized water 3 times, then redisperse it in deionized water to prepare a nano-calcium carbonate suspension with a concentration of 30mg / mL.

[0065] The morphology, particle size and surface potential of na...

Embodiment 2

[0075] A gelatin composite embolic microsphere containing nanometer calcium carbonate, the preparation method of the microsphere specifically comprises:

[0076] 1) Preparation of nano-calcium carbonate suspension:

[0077] 1-1) Weigh 20mM polyacrylic acid (molecular weight 2000Da) and 20mM calcium chloride and dissolve in 200mL deionized water, stir at room temperature for 1h to obtain a mixed solution;

[0078] 1-2) Dissolve 20mM sodium carbonate in 200mL of deionized water, then quickly add it to the mixed solution obtained in step 1), and stir at room temperature for 1h;

[0079] 1-3) After the reaction, centrifuge at 12000rpm for 15min to get the precipitate, wash the resulting precipitate with deionized water 3 times, then redisperse it in deionized water to prepare a nano-calcium carbonate suspension with a concentration of 30mg / mL.

[0080] The morphology, particle size and surface potential of nano-calcium carbonate were characterized by transmission electron microsc...

Embodiment 3

[0103] Example 3: Determination of drug loading of doxorubicin by gelatin composite microspheres containing nano-calcium carbonate

[0104] Prepared the gelatin composite microsphere containing nano-calcium carbonate according to the method of embodiment 1, with the common gelatin microsphere (being the step 2 of embodiment 1)) that does not contain nano-calcium carbonate prepared by the same method as contrast, common gelatin microsphere The preparation method is:

[0105] Add 500mg of gelatin to 5mL of deionized water, heat to 55°C and stir to dissolve the gelatin completely to obtain an aqueous gelatin solution, namely the water phase;

[0106] Measure 50mL of liquid paraffin, add Span 80 with a mass fraction of 1%, heat to 55°C and stir to form an oil phase;

[0107] Under 1200rpm magnetic stirring, the obtained water phase was dropped into the obtained oil phase, and emulsified for 30min; 25% glutaraldehyde solution, continue to stir, react and crosslink for 1 hour;

...

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Abstract

The invention discloses gelatin composite embolism microspheres containing nano calcium carbonate, a preparation method of the gelatin composite embolism microspheres and drug-loaded embolism microspheres. The microspheres comprise the following components in percentage by mass: 10-50% of nano calcium carbonate and 50-90% of gelatin. The gelatin composite microsphere containing the calcium carbonate nanoparticles provided by the invention can efficiently adsorb an anti-tumor chemotherapeutic drug through good water absorption of gelatin and extremely strong negative charge property of the calcium carbonate nanoparticles, and can realize long-time stable release of the drug, thereby playing a long-acting anti-tumor role; in the tumor embolism treatment process, the microspheres can continuously maintain a slightly alkaline environment of tumor tissues for a long time, so that the protective effect of lactic acid on tumor cells is effectively eliminated, and the sensitivity of chemotherapeutic drugs is improved; meanwhile, the embolism microsphere provided by the invention is simple in preparation method, relatively low in cost and good in reproducibility, has good biological safety and degradability, and has a bright application prospect in the field of liver cancer TACE treatment.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a gelatin composite embolic microsphere containing nano-calcium carbonate, a preparation method thereof and drug-loaded embolic microspheres. Background technique [0002] Hepatocellular carcinoma (HCC) is a malignant tumor with high incidence worldwide, which seriously threatens the life and health of our people. HCC has the characteristics of insidious onset, high degree of malignancy and rapid progression. More than 70% of patients are in the middle and late stage when they are clinically diagnosed, and the best time for surgical resection is lost. For patients with advanced liver cancer, interventional comprehensive therapy based on transcatheter arterial chemoembolization (TACE) has shown excellent anti-tumor efficacy, and has gradually become the first choice for clinical treatment of advanced liver cancer. The main principle of TACE is to block tumor blood supply arter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/02A61L24/10A61L24/00
CPCA61L24/02A61L24/104A61L24/001A61L24/0015A61L2400/12A61L2300/416A61L2300/602
Inventor 纪建松殳高峰陈敏江赵中伟陈为谦郭小菊陈晓晓乔恩齐
Owner LISHUI CENT HOSPITAL
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