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WGX-50 liposome as well as preparation method and application thereof

A technology of WGX-50 and liposome, which is applied in the fields of health care products, medicine and cosmetics, can solve problems such as poor applicability, poor water solubility, and easy crystallization, and achieve the goal of improving application potential, lipid stability, and water solubility Effect

Active Publication Date: 2022-03-25
上海彤颜实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of poor water solubility and poor applicability of WGX-50 in the prior art, liposomes prepared by conventional methods also have problems such as easy aggregation and crystallization, the present invention provides a WGX-50 liposome, a preparation method and application

Method used

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  • WGX-50 liposome as well as preparation method and application thereof
  • WGX-50 liposome as well as preparation method and application thereof
  • WGX-50 liposome as well as preparation method and application thereof

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preparation example Construction

[0036] The present invention provides a kind of preparation method of WGX-50 liposome, comprising:

[0037] S1. Dissolving:

[0038] Disperse WGX-50, phospholipids and phytosterols in an organic solvent according to the above-mentioned preset ratio, and ultrasonically dissolve;

[0039] Wherein, the organic solvent is one of ethanol, ethyl acetate and chloroform, and the ultrasonic dissolution time is 1 to 4 minutes, preferably 2 minutes;

[0040] S2, evaporation:

[0041] At the preset temperature, the organic solvent was removed by slow rotary evaporation;

[0042] Wherein, the evaporation temperature is 30-50°C, preferably 40°C; the rotation speed of the rotary evaporator is 60-100rpm, preferably 80rpm;

[0043] S3, vacuum drying:

[0044] Vacuum drying at room temperature to further remove a small amount of residual organic solvent to form a dry film;

[0045] Wherein, the vacuum drying time is 20 to 40 minutes, preferably 30 minutes;

[0046] S4, hydration treatment...

Embodiment 1

[0057]Accurately weigh 80mg of soybean lecithin, 10mg of phytosterol oleate and 8mg of WGX-50 in a 50mL flask, add 20mL of absolute ethanol, ultrasonically dissolve for 2min, place it in a rotary evaporator at 40°C, evaporate slowly at 80rpm to remove anhydrous Ethanol, a layer of uniform film was formed on the bottle wall, after vacuum drying at room temperature for 30 minutes, 10 mL of deionized water and 20 mg of bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylate were added, and the lipid was obtained by rotating and hydrating for 30 minutes. The plastid suspension was transferred to a cell disintegrator and sonicated for 10 minutes, and 10 mL of aqueous solution containing 50 mg of chitin was added to the obtained clear solution, and after mixing and stirring for 30 minutes, a uniform and transparent WGX-50 liposome solution was obtained.

Embodiment 2

[0059] Accurately weigh 80mg of soybean lecithin, 10mg of phytosterol / octyldodecyl lauroyl glutamate and 8mg of WGX-50 in a 50mL flask, add 20mL of absolute ethanol, ultrasonically dissolve for 2min, and place in a 40°C rotary evaporator At 80rpm, the ethanol was slowly evaporated to remove anhydrous ethanol, and a uniform film was formed on the bottle wall. After vacuum drying at room temperature for 30min, 10mL of deionized water and 20mg of bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylate were added. Ester, rotate and hydrate for 30 minutes to obtain a liposome suspension, move it to a cell disintegrator and sonicate for 10 minutes, add 10 mL of an aqueous solution containing 50 mg of chitin to the obtained clear solution, and mix and stir for 30 minutes to obtain uniform and transparent WGX-50 Liposome solution.

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Abstract

The invention discloses a WGX-50 liposome as well as a preparation method and application thereof, and the WGX-50 liposome is prepared from WGX-50, phospholipids, phytosterols, a lipid structure modifier, a stabilizing additive and deionized water. The preparation method comprises the following steps: dispersing the WGX-50, the phospholipids and the phytosterols in an organic solvent; carrying out rotary evaporation and vacuum drying to remove the organic solvent and form a dry film; adding a stabilizing additive and deionized water into the dry film, and carrying out hydration treatment and micronization treatment; and adding an aqueous solution of a lipid structure modifier into the solution obtained by micronization treatment, and mixing and stirring to obtain the WGX-50 liposome. WGX-50 is wrapped in a liposome form, so that the water solubility of WGX-50 can be effectively improved, and the application potential of WGX-50 can be improved; meanwhile, through combined use of the phytosterols, the lipid structure modifier and the stability additive, the stability of the prepared WGX-50 lipid is remarkably improved.

Description

technical field [0001] The invention relates to the technical fields of cosmetics, health care products and medicine, in particular to a WGX-50 liposome, a preparation method and application. Background technique [0002] Xanthoxylin WGX-50 is a kind of natural ingredients in the natural condiment Zanthoxylum bungeanum. It has potential moisturizing, anti-oxidation and other cosmetic functions, and can be used in the development of various cosmetics. After research, WGX-50 also has a specific gene regulation effect, which can effectively inhibit the aggregation of Aβ amyloid protein in the human brain (the aggregation of Aβ amyloid protein in the brain is considered to be an important pathological feature of Alzheimer's disease) and The expression of the lipid cell apoptosis protein can reduce cell apoptosis, thereby effectively protecting nerve cells, and thus has the potential application of preparing anti-Alzheimer's disease drugs. In 2010, Wei Dongqing and others used 3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/165A61K47/10A61K47/14A61K47/24A61K47/28A61K47/36A61K8/14A61K8/34A61K8/37A61K8/42A61K8/73A23L33/105A23L29/00A23L29/30A23P10/30A61P25/28A61Q19/00
CPCA61K9/1277A61K31/165A61K47/24A61K47/28A61K47/36A61K47/10A61K47/14A61K8/14A61K8/42A61K8/736A61K8/345A61K8/37A61Q19/00A61P25/28A23L33/105A23L29/05A23L29/045A23L29/04A23L29/30A23L29/035A23P10/30A61K2800/10A23V2002/00A23V2200/30A23V2250/21A23V2250/1842A23V2250/511A23V2300/10A23V2300/48Y02A50/30
Inventor 琚建伟张守兵丁保安
Owner 上海彤颜实业有限公司
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