Anion diagnosis marker for osteoporosis and detection method thereof

A diagnostic marker and osteoporosis technology, which is applied in the field of osteoporosis negative ion diagnostic markers and its detection, can solve the problems of limited detection and achieve a wide range of applications

Active Publication Date: 2022-03-22
川北医学院附属医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned problems existing in the prior art, the present invention provides a negative ion diagnostic marker for osteoporosis and a detection method thereof. The negative ion marker can accurately reflect whether a patient suffers from osteoporosis, and has the advantages of convenient detection, The advantage of no radiation damage to the human body can effectively solve the problem of limited detection in the existing dual-energy X-ray absorptiometry

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  • Anion diagnosis marker for osteoporosis and detection method thereof
  • Anion diagnosis marker for osteoporosis and detection method thereof

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Embodiment 1

[0026] A negative ion diagnostic marker for osteoporosis, including two kinds of serum polar negative ions, the two serum polar negative ions are respectively 4-hydroxyproline and 3-[3-(sulfoxyl)phenyl]propionic acid, The detection method of this marker is as follows:

[0027] (1) Take 50 μL serum sample and place it in a 96-well plate, add 200 μL methanol / acetonitrile (1:1, v / v);

[0028] (2) Vortex for 5 minutes, let stand at 4°C for 10 minutes, then centrifuge in a plate centrifuge at 5010g for 20 minutes at 4°C;

[0029] (3) After transferring 200 μL of the supernatant to a new 96-well plate, freeze-dry in a freeze dryer for 2-3 hours;

[0030] (4) sealed and stored at low temperature for mass spectrometry analysis;

[0031] (5) Reconstitute the lyophilized supernatant with 80 μL of acetonitrile-water (1:3, v / v), vortex for 3 minutes, centrifuge at 5010 g for 20 minutes at 4°C, and then take 70 μL of the supernatant for testing , the specific detection process is: using...

Embodiment 2

[0034] A negative ion diagnostic marker for osteoporosis, including two kinds of serum polar negative ions, the two serum polar negative ions are respectively 4-hydroxyproline and 3-[3-(sulfoxyl)phenyl]propionic acid, The detection method of this marker is as follows:

[0035] (1) Take 50 μL serum sample and place it in a 96-well plate, add 200 μL methanol / acetonitrile (1:1, v / v);

[0036] (2) Vortex for 5 minutes, let stand at 4°C for 10 minutes, then centrifuge at 4800g for 20 minutes at 5°C in a plate centrifuge;

[0037] (3) After transferring 200 μL of the supernatant to a new 96-well plate, place it in a freeze dryer for 2 hours to freeze-dry;

[0038] (4) sealed and stored at low temperature for mass spectrometry analysis;

[0039] (5) Reconstitute the lyophilized supernatant with 80 μL of acetonitrile-water (1:3, v / v), vortex for 3 minutes, centrifuge at 5000 g for 15 minutes at 4°C, and then take 70 μL of the supernatant for sample detection , the specific detectio...

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Abstract

The invention provides a negative ion diagnostic marker for osteoporosis and a detection method thereof, the marker comprises two kinds of serum polar negative ions, and the two kinds of serum polar negative ions are respectively 4-hydroxyproline and 3-[3-(sulfoxy) phenyl] propionic acid. The marker can accurately reflect whether a patient suffers from osteoporosis or not, has the advantages of being convenient to detect and free of radioactive damage to a human body, and can effectively solve the problem that an existing dual-energy X-ray absorption method is limited in detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a negative ion diagnostic marker for osteoporosis and a detection method thereof. Background technique [0002] With the rapid increase of the global aging population, the incidence of osteoporosis is also increasing year by year. Osteoporosis is a skeletal disease characterized by decreased bone mass and microstructural deterioration leading to increased bone fragility, often accompanied by long-term chronic pain, deformity, disability, and varying degrees of limited activity, which seriously affects the lives of the elderly Quality and Mental Health. At present, early diagnosis and early treatment are the main treatment strategies for osteoporosis. However, osteoporosis is a highly occult disease and lacks specific and sensitive biomarkers. Many patients are diagnosed after fractures occur. At present, the determination value of dual-energy X-ray absorptiometry (DXA)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/72G01N33/68
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/7266G01N33/6893G01N33/6848G01N33/6812G01N2030/324G01N2800/108
Inventor 邓大炜曾丽娟潘晨韦天夫
Owner 川北医学院附属医院
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