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Construction method, detection method and application of fingerprint spectrum of Juanbi decoction composition

A technology of fingerprint spectrum and construction method, which is applied in the field of construction of the fingerprint spectrum of Juanbi Decoction composition, can solve the problem that there is no literature report on the composition of Juanbi Decoction, and achieves guaranteeing stability and adaptability, controlling internal quality, and controlling preparations. quality effect

Active Publication Date: 2022-03-18
SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems existing in the prior art, the present invention provides a method for constructing and detecting the fingerprint of the composition of Juanbi Decoction, aiming to solve the defect that there is no literature report on the fingerprint of the composition of Juanbi Decoction in the prior art, Effectively Controlling the Quality of Juanbi Decoction Preparation

Method used

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  • Construction method, detection method and application of fingerprint spectrum of Juanbi decoction composition
  • Construction method, detection method and application of fingerprint spectrum of Juanbi decoction composition
  • Construction method, detection method and application of fingerprint spectrum of Juanbi decoction composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, the preparation of reference substance solution

[0074] Take loganinic acid, molysin A, chlorogenic acid, gentiopicroside, ferulic acid, cinnamonin, cinnamic aldehyde, ammonium glycyrrhizinate, ligustilide, osthole, 11-carbonyl-β - An appropriate amount of acetylboswellic acid reference substance, accurately weighed, respectively added methanol aqueous solution with a volume fraction of 80% to prepare each 1mL containing 46.44 μg of loganinic acid, 47.38 μg of mulberryside A, 51.76 μg of chlorogenic acid, gentian 50.75 μg of picroside, 90.24 μg of ferulic acid, 50.42 μg of proscurdin, 88.65 μg of cinnamon aldehyde, 95.68 μg of ammonium glycyrrhizinate, 99.81 μg of ligustilide, 55.25 μg of osthole, 11-carbonyl-β-acetyl boswellia A mixed solution of 51.67 μg of acid was used as the reference substance solution of the reference substance.

Embodiment 2

[0075] Embodiment 2, the preparation of Juanbi Decoction composition

[0076] Take 3.73kg notopterygium, 3.73kg duhuo, 1.87kg cinnamon, 3.73kg Gentiana, 11.19kg angelica, 2.61kg Chuanxiong, 1.87kg honey-roasted licorice, 7.46kg Caulis chinensis, 11.19kg mulberry branch, 2.98kg frankincense, 2.98kg woody incense, A total of eleven decoction pieces, add water to decoct twice, add 533kg water for the first time, decoct for 1 hour, add 425kg water for the second time, decoct for 1 hour, combine the two extracts, filter with a 200-mesh filter cloth while hot, The filtrate was concentrated under reduced pressure, dried, pulverized, and granulated to obtain the Juanbi Decoction composition.

Embodiment 3

[0077] Embodiment 3, the investigation of fingerprint spectrum detection wavelength

[0078] Use octadecylsilane bonded silica gel as filler; use acetonitrile as mobile phase A, and use phosphoric acid aqueous solution with a volume fraction of 0.1% as mobile phase B, carry out gradient elution according to the provisions in Table 1; flow rate 0.4mL / mL, column temperature: 30°C.

[0079] Table 1: Mobile Phase Gradient Program for Fingerprinting

[0080] time (minutes) Acetonitrile (%) Phosphoric acid aqueous solution (%) with a volume fraction of 0.1% 0～8 8→11 92→89 8～15 11→17 89→83 15～20 17→22.5 83→77.5 20～30 22.5→42.5 77.5→57.5 30～36 42.5→61 57.5→39 36～40 61→100 39→0

[0081] Adopt diode array detector to carry out full-wavelength detection to need testing solution, and collect the collection of illustrative plates at 220nm, 254nm, 300nm, 330nm wavelength place simultaneously (see Figure 1~2 ), comprehensive investiga...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a construction method, a detection method and application of a fingerprint spectrum of a Juanbi decoction composition. The construction method of the fingerprint spectrum comprises the following steps: taking a Juanbi decoction composition, adding an extraction solvent, weighing, carrying out ultrasonic treatment, cooling, weighing again, complementing the lost weight by using the extraction solvent, uniformly shaking, filtering, and taking a subsequent filtrate to obtain a test solution; 11 reference substances such as loganic acid, mulberroside A, chlorogenic acid and gentiopicroside are taken and prepared into reference substance solutions of the reference substances; respectively and precisely sucking 1-2mu L of the reference solution and the test solution, injecting the reference solution and the test solution into a liquid chromatograph for chromatographic analysis, measuring and recording chromatograms to respectively obtain a test fingerprint spectrum and a reference chromatogram spectrum, and formulating a standard fingerprint spectrum of the Juanbi decoction composition. According to the present invention, the quality information of the Juanbi decoction can be comprehensively reflected by the fingerprint so as to achieve the purpose of more comprehensively and effectively controlling the quality of the Juanbi decoction preparation product.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a construction method, a detection method and an application of a Juanbi Decoction fingerprint composition. Background technique [0002] Juanbi Decoction comes from the chapter "Bi (Crane's Knee Wind)" in volume three of the comprehensive medical book "Medical Mind Wu" written by Cheng Guopeng in the Qing Dynasty. "To cure the three qi of wind, cold and dampness, and combine them to form numbness." The composition is Qianghuo and Duhuo 1 qian each, Guixin 5 qian, Gentiana qin 1 qian, angelica 3 qian, Chuanxiong 70 qian, licorice 5 qian (burned), Caulis chinensis 2 qian, mulberry branch 3 qian, frankincense and woody incense 8 qian each . Indications for apoplexy, body aches and pains, stiff neck and back, numbness in hands and feet, heavy waist and knees, and difficulty in moving. In the prescription, Qianghuo, Duhuo, Gentiana chinensis, and cinnamon dispel wind ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/8631G01N30/8637Y02A50/30
Inventor 曾杉陈锦霞林碧珊高永坚陈伟钢张汝莹刘勇汤春花
Owner SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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