Kit for removing and detecting mycoplasma and application thereof

Abstract

The invention relates to a kit for removing and detecting mycoplasma and application of the kit. The kit comprises 0.5%-1% of an Opti-MEM solution of gatifloxacin, 0.5%-1% of telithromycin and 0.5%-1% of minocycline, wherein the pH value of the Opti-MEM solution is 5.0-7.0. According to the kit, the mycoplasma can be cleared more quickly and more effectively; and for various mycoplasmas, the specificity of the base sequence of the mycoplasmas is utilized, so that whether the mycoplasmas are contained or not can be detected more quickly and accurately.

Description

technical field [0001] The invention relates to a kit for removing and detecting mycoplasma and its application, belonging to the field of biotechnology. Background technique [0002] Mycoplasma is a prokaryotic organism with a size of only 0.2-0.3um, no cell wall, and can pass through ordinary filter membranes (0.22-0.45um). Gram staining is negative, but it is not easy to stain. It is generally stained with Giemsa and stained lavender . Mycoplasma mainly reproduces by binary fission, and can also reproduce by budding. The branches form filaments and then break into club-shaped particles. Most mycoplasmas reproduce slower than bacteria, the optimum growth temperature is 35°C, and the optimum pH value is 7.8-8.0. Cultivated on solid medium to form typical "poached egg" colonies. Mycoplasma has weak resistance and is sensitive to heat and dryness. [0003] In the process of cell culture, the incidence of mycoplasma infection reaches 63%, so the contamination by mycoplasma...

AI Technical Summary

Problems solved by technology

However, there are two main disadvantages: first, the mycoplasma removal cycle is long and cannot be completely removed; second, the detection specificity of mycoplasma is poor, the speed is slow, and the accuracy rate is low

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