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Application of reagent for detecting expression level of RIG-I-Shart in preparation of products for tumor diagnosis and/or prognosis

An expression level, tumor diagnosis technology, applied in expression level reagents, application fields in products

Active Publication Date: 2022-03-01
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is a lack of a biomarker capable of diagnosing and prognosing the sensitivity of tumor cells to immunotherapy

Method used

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  • Application of reagent for detecting expression level of RIG-I-Shart in preparation of products for tumor diagnosis and/or prognosis
  • Application of reagent for detecting expression level of RIG-I-Shart in preparation of products for tumor diagnosis and/or prognosis
  • Application of reagent for detecting expression level of RIG-I-Shart in preparation of products for tumor diagnosis and/or prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 RIG-I-Short is an alternative cut form of RIG-I induced by interferon

[0056] (1) Identification of RIG-I-Short protein sequence and mRNA sequence

[0057] RIG-I is a typical interferon-stimulated gene, and its expression will be up-regulated under virus infection and interferon-stimulated conditions. This result is verified by the present invention through Western blotting and quantitative real-time PCR experiments. The results of gel electrophoresis experiments showed that: in human cells, the RIG-I N-terminal specific antibody can recognize a protein located at a size of 17kDa ( figure 1 ).

[0058] In order to further confirm that the protein corresponding to the RIG-I N-terminal antibody recognition band is the RIG-I subtype, the present invention infected H460 cells with Sendai virus, and used the RIG-I N-terminal antibody to enrich the protein at 24 hours and 48 hours, and passed Protein gel electrophoresis separated proteins of different sizes, and ...

Embodiment 2

[0067] Example 2 Physiological and pathological functions of RIG-I-Short

[0068] (1) RIG-I-Short inhibits the activity of transcription factor STAT1

[0069] In order to explore the physiological function of RIG-I-Short, the present invention uses the method of FLAG pμlldown combined with mass spectrometry to identify proteins interacting with RIG-I-Short, and finds that the transcription factor STAT1, which plays an important role in the interferon pathway, can interact with RIG-I -Short combination ( Figure 15 ). Subsequently, the present invention verified the interaction between RIG-I-Short and STAT family members, and co-immunoprecipitation experiments revealed that RIG-I-Short interacted with STAT1 and STAT2 ( Figure 16 ).

[0070]When the interferon binds to the interferon receptor, it recruits downstream kinases to phosphorylate STAT1, and the phosphorylated STAT1 forms a dimer, and then enters the nucleus to exert its transcription factor activity. In order to ...

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Abstract

The invention provides application of a reagent for detecting the expression level of RIG-I-Sport in preparation of a product for tumor diagnosis and / or prognosis, and belongs to the technical field of biology. According to the present invention, the protein sequence of the RIG-I-Sport is represented by SEQ ID No. 1; rIG-I-Shart is a variable spliceosome of RIG-I, and expression of MHC I type molecules is reduced by inhibiting nuclear translocation of a transcription factor STAT1. According to the invention, high expression of RIG-I-Shart in tumor tissues of colorectal patients is found. The invention discovers that the tumor cells overexpressing RIG-I-Shart can escape from the attack of the host immune system, thereby promoting the growth of the tumor. The invention finds that the sensitivity of tumor cells overexpressing RIG-I-Shart to immunotherapy such as tumor vaccines and the like is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a reagent for detecting the expression level of RIG-I-Short in the preparation of products for tumor diagnosis and / or prognosis. Background technique [0002] RIG-I (retinoic acid-inducible gene-I) is a double-stranded RNA sensor located in the cytoplasm. The RIG-I protein consists of 925 amino acids, with a molecular weight of about 106kDa, including two CARD structures at the N-terminus, two RNAHelicase domains in the middle, and a CTD domain at the C-terminus. In the resting state, the CARD domain at the N-terminal of RIG-I is folded inside and cannot function. During virus infection, RIG-I binds to viral double-stranded RNA and exposes the CARD domain. The exposed N-terminal RIG-I can undergo K63-type ubiquitination and interact with MAVS localized in mitochondria, thereby activating the transcription factor IRF3 and promoting the transcription of type-1 inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N9/14C12N15/55
CPCC12Q1/6886C12N9/14C12Y306/04013C12Q2600/158C12Q2600/118Y02A50/30
Inventor 尹玉新吕丹宋佳
Owner PEKING UNIV
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