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Method for preventing and controlling aspergillus flavus and toxin thereof and increasing quantity of nitrogenase active root nodules at roots of leguminous crops and application of method

A technology of aflatoxin and Aspergillus flavus, which is applied in the fields of preventing and controlling Aspergillus flavus and its toxins and increasing the number of nitrogenase activity root nodules in the roots of leguminous crops and its application, so as to achieve easy popularization and application, strong practicability, and increase crop production Effect

Active Publication Date: 2022-03-01
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to increase the number of root nodules in crops, a lot of research work has focused on rhizobia to develop excellent rhizobia, but currently there are few that can be applied to production and play a significant role

Method used

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  • Method for preventing and controlling aspergillus flavus and toxin thereof and increasing quantity of nitrogenase active root nodules at roots of leguminous crops and application of method
  • Method for preventing and controlling aspergillus flavus and toxin thereof and increasing quantity of nitrogenase active root nodules at roots of leguminous crops and application of method
  • Method for preventing and controlling aspergillus flavus and toxin thereof and increasing quantity of nitrogenase active root nodules at roots of leguminous crops and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Nanobody Preparation of Toxicity Indicator Molecule AFT-YJFZ01 Produced by Aflatoxin-producing Bacteria

[0072] Using AFT-YJFZ01 as the immune antigen, immunizing alpaca or Balb / c mice in a conventional way, and then using the known conventional nanobody or mouse monoclonal antibody preparation technology, it can be developed and obtained.

[0073]Dissolve the AFT-YJFZ01 prepared above in conventional PBS buffer or normal saline to a concentration of not less than 0.1mg / mL, then mix and emulsify with Freund's complete adjuvant in equal volume, and inject it subcutaneously or intradermally at multiple points on the back Immunize the alpaca, and then boost the immunization once every 2-4 weeks, and replace the Freund's complete adjuvant with Freund's incomplete adjuvant during the booster immunization. Use conventional ELISA process to monitor the immune effect, and after the alpaca serum titer no longer rises, then take blood from the venous blood of the immun...

Embodiment 2

[0075] Example 2 Preparation of Monoclonal Antibody to Toxicity Indicator Molecule AFT-YJFZ01 of Aflatoxin-producing Bacteria

[0076] Using AFT-YJFZ01 as the immune antigen, immunizing alpaca or Balb / c mice in a conventional way, and then using the known conventional nanobody or mouse monoclonal antibody preparation technology, it can be developed and obtained.

[0077] Dissolve the AFT-YJFZ01 prepared above in conventional PBS buffer or normal saline to a concentration of not less than 0.1mg / mL, then mix and emulsify with Freund's complete adjuvant in equal volume, and inject it subcutaneously or intradermally at multiple points on the back BALB / c mice were then boosted every 2-4 weeks, and Freund's complete adjuvant was replaced with Freund's incomplete adjuvant during the booster immunization. Use conventional ELISA process to monitor the immune effect, and after the serum titer of BALB / c mice no longer rises, then isolate the splenocytes of immunized mice, splenocytes are...

Embodiment 3

[0079] Example 3 Preparation of Rabbit-derived Polyclonal Antibody of Toxicity Indicator Molecule AFT-YJFZ01 Produced by Aflatoxin-producing Bacteria

[0080] Using AFT-YJFZ01 as the immune antigen, the New Zealand white rabbit and other test rabbits are immunized in a conventional way, and then the known conventional rabbit polyclonal antibody preparation technical scheme can be used to develop and obtain it.

[0081] The AFT-YJFZ01 prepared above was directly used as an antigen, mixed and emulsified with equal volumes of Freund's complete adjuvant with a solution of not less than 0.1 mg / mL, and injected subcutaneously or intradermally into New Zealand white rabbits at multiple points on the back, and then Boost immunization once every 2-4 weeks, and replace complete Freund's adjuvant with Freund's incomplete adjuvant during booster immunization. The immunization effect is monitored by conventional ELISA process. After the serum titer of immunized animals no longer rises, the...

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Abstract

The invention relates to a method for preventing and controlling aspergillus flavus and toxin thereof and increasing the number of nitrogenase active root nodules of leguminous roots. Comprising the following steps: obtaining bacillus amyloliquefaciens, brevibacillus laterosporus, bacillus mucilaginosus and enterobacter ludwigii which have prevention and control effects on aspergillus flavus and toxins thereof; after two or three of bacillus amyloliquefaciens, brevibacillus laterosporus and bacillus mucilaginosus are mixed with enterobacter ludwigii, the mixture is applied to the field of leguminous crops, and the number of root nodules with nitrogenase activity at the roots of the leguminous crops is increased. The strain is used for increasing the number of nitrogenase active root nodules at the roots of leguminous crops such as peanuts and reducing the levels of aspergillus flavus and aflatoxin, and is simple to use, remarkable in yield increasing benefit and ecological benefit and easy to popularize and apply.

Description

technical field [0001] The invention relates to a method for preventing and controlling Aspergillus flavus and its toxins in leguminous crops and increasing the number of active nodules of nitrogenase in the roots of leguminous crops and its application. Background technique [0002] Aflatoxin is highly toxic and harmful, and it is the pollutant that contaminates the most types of food. my country is one of the countries with the most serious aflatoxin pollution in the world. In recent years, the overall trend of pollution has been aggravated, seriously threatening food safety and people's health. Aflatoxin is the most toxic mycotoxin in nature, among which aflatoxin B1 is a Class I carcinogen identified by the International Agency for Research on Cancer (IARC), which has caused many poisoning incidents of human and animal groups , has become one of the main reasons for the high incidence of liver cancer cases. According to the statistics retrieved by Web of Science in the ...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G22/40C12N1/20C12R1/07C12R1/01
CPCA01G7/06A01G22/40C12N1/20
Inventor 张奇李培武岳晓凤
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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