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A kind of tissue culture method of Buglossia cordata jackfrost

A technology for tissue culture and oxglossus, which is applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., and can solve the limitation of application of JackFrost, low reproductive efficiency, and the reproductive efficiency of J. Low problems, to achieve the effect of vigorous tissue cell division, improve reproduction efficiency, and improve the success rate of induction

Active Publication Date: 2022-06-24
BEIJING GREEN GARDEN GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the propagation methods of Buglossia cordata Jack Frost are mainly seed propagation and branch propagation. The above-mentioned propagation methods are relatively traditional, and their reproduction efficiency is low.
In addition, Jack Frost should avoid high temperature, high humidity, and long-term direct exposure to strong light. Therefore, the reproductive efficiency of Jack Frost is also affected by seasonal changes. Reproductive efficiency is lower in hot seasons
[0004] In view of the related technologies mentioned above, the inventor believes that, on the one hand, the traditional breeding technology that Jack Frost Cordica relies on currently makes the reproductive efficiency of Buglossia Cardiac Jack Frost relatively low, so it relies on Buglossia Cardiac The current breeding technology of JackFrost obviously limits the application of Buglossia cordata Jack Frost; on the other hand, under the influence of seasonal changes, the reproductive efficiency of Buglossia cordata Jack Frost becomes lower, which further limits the Application of Buglossia cordata Jack Frost

Method used

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  • A kind of tissue culture method of Buglossia cordata jackfrost
  • A kind of tissue culture method of Buglossia cordata jackfrost
  • A kind of tissue culture method of Buglossia cordata jackfrost

Examples

Experimental program
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Effect test

Embodiment 1

[0061] The present embodiment provides a tissue culture method of Jack Frost, which specifically includes the following steps:

[0062] 1. Acquisition and Sterilization of Explants

[0063] (1) Acquisition of explants: the mother plant is placed in a greenhouse for 35 days, withered and diseased leaves are removed in the process, and a preventive fungicide is sprayed every 15 days; Mother plant, select new leaves that are fully expanded and free from diseases and insect pests on the mother plant;

[0064] (2) Sterilization of explants:

[0065] Rinse: rinse the new leaves obtained in step (1) with running tap water for 10 minutes, and dry them in the air;

[0066] Alcohol disinfection: After drying, soak the new leaves in 75% alcohol for 30s, then shake and wash them with sterile water for 1-2 minutes each time;

[0067] 0.1% mercuric chloride treatment: the new leaves are then soaked in 0.1% mercuric chloride solution and shaken for 5 minutes, and then shaken and washed wi...

Embodiment 2-3

[0085] Embodiment 2-3 provides a kind of tissue culture method of Heliotrope chinensis Jack Frost respectively, and the difference between it and Example 1 lies in the parameters in each step and the medium formula used, specifically as shown in Table 1. Show.

Embodiment 4-5

[0090] Embodiment 4-5 provides a kind of tissue culture method of Heliotropa chinensis Jack Frost respectively, and the difference between it and embodiment 2 is the growth phase of the new leaf as explant on the mother plant, as shown in the table. 2 shown.

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Abstract

This application relates to the technical field of in vitro cultivation of plants, and specifically discloses a kind of Buglossia cordata Jack Frost tissue culture methods. Concretely include the following steps: obtaining and disinfection of explants, inducing explants to form callus and adventitious buds, proliferation of adventitious buds and inducing adventitious buds to take root; wherein, in the obtaining and disinfection steps of explants, the explants are selected The new leaves of the mother plant that grow vigorously and are free from diseases and insect pests; and provide the medium formula used in inducing explants to form callus and adventitious buds, adventitious bud proliferation, and inducing adventitious buds to take root. This application can effectively improve the Jack Frost The reproductive efficiency of Buglossia cordata Jack Frost Applications.

Description

technical field [0001] The present application relates to the technical field of plant culture in vitro, more specifically, it relates to a tissue culture method of JackFrost. Background technique [0002] Jack Frost (Brunnera macrophyllaJack Frost), belonging to the genus Brunnera macrophylla, is a perennial herb, clustered, hemispherical; the leaves are oval heart-shaped, and the leaf color is frosty silver with green veins ; Leaf surface and leaf back densely covered with white pubescent hairs. This variety of plants has high ornamental value and is suitable for the foreground arrangement of flower borders in shade places, stream waterscape planting, roadside cluster planting, private garden courtyard planting and potted ornamental. It has a wide range of applications and has good market prospects. [0003] Jack Frost likes a cool, humid climate, likes loose, well-drained sandy loam, tolerates semi-shade, and grows well in shaded conditions such as under trees. At prese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 高丽辛培培杨聪儿石俊刘小妹王芳钱蕾
Owner BEIJING GREEN GARDEN GRP CO LTD
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