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Cancer marker screening method based on methylation enhanced gene expression (MEGE)

A screening method and gene expression technology, applied in the field of biomedicine, can solve problems such as poor sensitivity and loss of radical resection, and achieve the effect of improving the detection rate

Pending Publication Date: 2022-02-22
BIOCHAIN BEIJING SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor sensitivity of existing markers, many cancer patients are often diagnosed in the middle and advanced stages, losing the chance of radical resection

Method used

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  • Cancer marker screening method based on methylation enhanced gene expression (MEGE)
  • Cancer marker screening method based on methylation enhanced gene expression (MEGE)
  • Cancer marker screening method based on methylation enhanced gene expression (MEGE)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0092] This embodiment takes lung cancer markers as an example, and includes the following steps:

[0093] (1) Obtain the first candidate gene

[0094] Through the Human Protein Atalas database, genes with large differences in transcription levels between lung cancer and other cancer tissues, that is, genes with significantly higher transcription levels in lung cancer tissues than other cancer tissues (P<0.01), were selected as the first candidate genes.

[0095] The specific operation is as follows:

[0096] Enter Human Protein Atalas https: / / www.proteinatlas.org / website, click "MENU", select "THE PATGOLOGY ATALAS" under the label "THE HUMAN PROTEOME", and click to enter;

[0097] Select a gene with a high expression level in lung cancer tissue, that is, click the icon indicating the lung to enter;

[0098] According to the transcriptome information, compared with other cancers, genes with increased transcription levels in lung cancer tissues were selected, a total of 97 g...

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Abstract

The invention provides a cancer marker screening method, which comprises the following steps: screening cancer markers based on tissue gene expression level differences between a target cancer and other cancers, and tissue gene expression level differences and methylation level differences between the target cancer and normal tissues, wherein the gene expression level difference between the target cancer and other cancer tissues indicates that the gene expression level of the marker in the target cancer is obviously higher than that of the marker in other cancer tissues, the gene expression level difference between the target cancer and the normal tissue indicates that the gene expression level of the marker in the target cancer is obviously higher than that in the normal tissue, and the methylation level difference between the target cancer and the normal tissue indicates that the methylation level of the marker in the target cancer is obviously higher than that of the marker in the normal tissue. The method disclosed by the invention is expected to be complementary with a conventional methylation marker, well improves the cancer detection rate, and has the advantages of accuracy, rapidness and high throughput.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for screening cancer markers based on methylation-enhanced gene expression (MEGE). Background technique [0002] Cancer has become the main killer of human beings. Early detection of cancer can dramatically improve cure rates and reduce the dire personal and financial costs to patients, their families and the healthcare system. Due to the poor sensitivity of existing markers, many cancer patients are often diagnosed in the middle and advanced stages, losing the opportunity for radical resection. [0003] The mainstream theory believes that in cancer cells, hypermethylation of some gene promoter regions will cause down-regulation of gene expression, thereby causing cancer. Therefore, these hypermethylated fragments can be used as markers for cancer screening. According to the latest theory, the methylation of some internal sites of genes will cause the upregulati...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/154C12Q2600/158
Inventor 聂海珍吴振朱真毓应彬欣韩晓亮
Owner BIOCHAIN BEIJING SCI & TECH
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