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Stem cell 3D culture microcarrier for enhancing inoculation activity and preparation method thereof

A technology for inoculating active and stem cells, which is applied in the field of stem cell culture and can solve problems such as difficult adsorption of stem cell seeds.

Pending Publication Date: 2022-02-22
华子昂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention overcomes the deficiency that the existing stem cell culture carrier is not easy to absorb stem cell seeds during inoculation, and invents a stem cell 3D culture microcarrier with enhanced inoculation activity and a preparation method thereof. The specific steps are as follows:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)

[0020] S1 Dissolve seryl arginine dipeptide in alkaline sodium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that the dipeptide is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0021] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with dipeptide to the sodium trimetaphosphate solution, and the dipeptide is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0022] S3 Fully wash to remove the cross-linking agent and uncross-linked small peptides, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;

[0023] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can...

Embodiment 2

[0029] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)

[0030] S1 Dissolve seryl arginyl lysine tripeptide in alkaline potassium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that the tripeptide is adsorbed on the surface and inner pores of collagen microcarriers for 3D culture of stem cells on the wall;

[0031] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with tripeptides to the sodium trimetaphosphate solution, and the tripeptides are cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0032] S3 Fully wash to remove the cross-linking agent and uncross-linked small peptides, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;

[0033] S4 The cross-linked collagen microcarriers for 3D cult...

Embodiment 3

[0039] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)

[0040] S1 Mix and dissolve seryl arginine dipeptide, threonyl lysine dipeptide and seryl histidine dipeptide in alkaline sodium carbonate solution, add collagen microcarriers for stem cell 3D culture and stir well, The mixed dipeptide is adsorbed on the surface of the collagen microcarrier for 3D culture of stem cells and the inner pore wall;

[0041] S2 Transfer the collagen microcarriers for 3D culture of stem cells fully adsorbed and mixed with dipeptides to the sodium trimetaphosphate solution, and the dipeptides are cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0042] S3 Fully wash to remove the cross-linking agent and uncross-linked dipeptide, and make the cross-linked collagen microcarrier for 3D culture of stem cells reach pH 7.2-7.4;

[0043] S4 The c...

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PUM

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Abstract

The invention provides a stem cell 3D culture microcarrier for enhancing inoculation activity and a preparation method thereof. The preparation method comprises the following steps: cross-linking amino acid with positive charges under physiological conditions to the stem cell 3D culture microcarrier in a small peptide form is carried out; when the stem cells are inoculated, the stem cells with negative charges are attracted by positive charges in the carrier and are automatically enriched to the stem cell 3D culture microcarrier; when the stem cells proliferate, the cross-linked small peptides are hydrolyzed to release amino acids and can be utilized by the stem cells.

Description

technical field [0001] The invention relates to the field of stem cell culture, in particular to a stem cell 3D culture microcarrier with enhanced inoculation activity and a preparation method thereof. Background technique [0002] There are many types of stem cells, which have been widely used in many fields such as disease treatment, medical beauty, anti-aging, and drug screening. Stem cell culture has become a bottleneck restricting the development of the entire stem cell industry chain, and stem cell incubators have become a development hotspot in the field of stem cell culture. According to the characteristics of stem cell culture devices, it can be divided into two categories: 2D culture and 3D culture. 2D culture is mainly carried out by enlarging the dish, changing liquid in batches, and supplying gas by diffusion method. 3D culture is mainly completed in a three-dimensional container, which can independently control the composition of the culture medium, can activ...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/0075C12N5/0062C12N2513/00C12N2531/00
Inventor 华子昂刘宝全朱美瑛万君兴竹添张建赵凯龙
Owner 华子昂
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