Stem cell 3D culture microcarrier with glucosamine reinforced inoculation activity and preparation method of stem cell 3D culture microcarrier
A technology of glucosamine and inoculation activity, which is applied to the field of 3D culture microcarriers of stem cells with enhanced inoculation activity of glucosamine and the preparation thereof, and can solve the problems of difficult adsorption of stem cell seeds and the like.
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Embodiment 1
[0018] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)
[0019] S1 Dissolve glucosamine in alkaline sodium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that glucosamine is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and on the inner channel wall;
[0020] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with glucosamine to sodium trimetaphosphate solution, and the glucosamine is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;
[0021] S3 Fully wash to remove the cross-linking agent and uncross-linked glucosamine, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;
[0022] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can be directly...
Embodiment 2
[0028] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)
[0029] S1 Dissolve glucosamine in alkaline potassium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that glucosamine is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and on the inner channel wall;
[0030] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with glucosamine to sodium trimetaphosphate solution, and the glucosamine is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;
[0031] S3 Fully wash to remove the cross-linking agent and uncross-linked glucosamine, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;
[0032] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can be direc...
Embodiment 3
[0038] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)
[0039] S1 Dissolve glucosamine in alkaline sodium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that glucosamine is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and on the inner channel wall;
[0040] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with glucosamine to sodium trimetaphosphate solution, and the glucosamine is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;
[0041]S3 Fully wash to remove the cross-linking agent and uncross-linked glucosamine, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;
[0042] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can be directly ...
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