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Stem cell 3D culture microcarrier with glucosamine reinforced inoculation activity and preparation method of stem cell 3D culture microcarrier

A technology of glucosamine and inoculation activity, which is applied to the field of 3D culture microcarriers of stem cells with enhanced inoculation activity of glucosamine and the preparation thereof, and can solve the problems of difficult adsorption of stem cell seeds and the like.

Pending Publication Date: 2022-02-22
华子昂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention overcomes the deficiency that the existing stem cell culture carrier is not easy to absorb stem cell seeds during inoculation, and invents a stem cell 3D culture microcarrier with glucosamine enhanced inoculation activity and a preparation method thereof. The specific steps are as follows:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)

[0019] S1 Dissolve glucosamine in alkaline sodium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that glucosamine is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and on the inner channel wall;

[0020] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with glucosamine to sodium trimetaphosphate solution, and the glucosamine is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0021] S3 Fully wash to remove the cross-linking agent and uncross-linked glucosamine, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;

[0022] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can be directly...

Embodiment 2

[0028] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)

[0029] S1 Dissolve glucosamine in alkaline potassium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that glucosamine is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and on the inner channel wall;

[0030] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with glucosamine to sodium trimetaphosphate solution, and the glucosamine is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0031] S3 Fully wash to remove the cross-linking agent and uncross-linked glucosamine, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;

[0032] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can be direc...

Embodiment 3

[0038] (1) Modification of the carrier for 3D culture of stem cells (the corresponding operations are all completed under sterile conditions)

[0039] S1 Dissolve glucosamine in alkaline sodium carbonate solution, add collagen microcarriers for 3D culture of stem cells and stir well, so that glucosamine is adsorbed on the surface of collagen microcarriers for 3D culture of stem cells and on the inner channel wall;

[0040] S2 transfers the collagen microcarriers for 3D culture of stem cells fully adsorbed with glucosamine to sodium trimetaphosphate solution, and the glucosamine is cross-linked and fixed on the surface of the collagen microcarriers for 3D culture of stem cells and the inner pore wall;

[0041]S3 Fully wash to remove the cross-linking agent and uncross-linked glucosamine, and make the cross-linked collagen microcarriers for 3D culture of stem cells reach pH 7.2-7.4;

[0042] S4 The cross-linked collagen microcarriers for 3D culture of stem cells can be directly ...

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Abstract

The invention provides a stem cell 3D culture microcarrier with glucosamine reinforced inoculation activity and preparation method of stem cell 3D culture microcarrier, wherein glucosamine is cross-linked to the stem cell 3D culture microcarrier through a linker capable of being subjected to enzymolysis, stem cells are adsorbed by means of the positive charge characteristic of the glucosamine, and enrichment of stem cell seeds on the carrier in the inoculation process is enhanced; after stem cells proliferate, specific lyase is used for cracking the stem cell 3D culture microcarrier, and glucosamine is dissociated along with the carrier and is eliminated through cleaning.

Description

technical field [0001] The invention relates to the field of stem cell culture, in particular to a stem cell 3D culture microcarrier with glucosamine enhanced inoculation activity and a preparation method thereof. Background technique [0002] There are many types of stem cells, which have been widely used in many fields such as disease treatment, medical beauty, anti-aging, and drug screening. Stem cell culture has become a bottleneck restricting the development of the entire stem cell industry chain, and stem cell incubators have become a development hotspot in the field of stem cell culture. According to the characteristics of stem cell culture devices, it can be divided into two categories: 2D culture and 3D culture. 2D culture is mainly carried out by enlarging the dish, changing liquid in batches, and supplying gas by diffusion method. 3D culture is mainly completed in a three-dimensional container, which can independently control the composition of the culture mediu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/0775
CPCC12N5/0075C12N5/0062C12N5/0662C12N2513/00C12N2531/00
Inventor 华子昂刘宝全张建朱美瑛万君兴竹添赵凯龙
Owner 华子昂
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