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Sample-residue-free heat-insulating and reusable multifunctional cell counting and imaging device

A cell counting and multi-functional technology, applied in the direction of measuring devices, laboratory containers, instruments, etc., can solve the problems of loss of excitation intensity, low efficiency of the experimental process, energy loss of light on the flow channel plate, etc., to achieve convenient positioning, The effect of convenient material setting

Active Publication Date: 2022-02-18
高分(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the occasions of optical imaging and spectral detection or some special requirements to obtain the flow velocity field distribution of microfluid, the existing technology almost without exception adopts external illumination and detectors arranged around the microfluidic device, which results in a On the one hand, the energy loss of light in the flow channel plate, resulting in the loss of excitation intensity, is unfavorable for the detection of weak signals
On the other hand, it is necessary to readjust the lighting and detection positions, making the experimental process inefficient and complicated

Method used

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  • Sample-residue-free heat-insulating and reusable multifunctional cell counting and imaging device
  • Sample-residue-free heat-insulating and reusable multifunctional cell counting and imaging device
  • Sample-residue-free heat-insulating and reusable multifunctional cell counting and imaging device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Such as figure 1 Shown is a top view of a four-microchannel heat-insulated reusable multifunctional cell counting imaging sample stage device of the present invention, including such as figure 2 As shown can cooperate with each other to form as figure 1 The first substrate 1 and the second substrate 2 of the shown four micro-channels, wherein the first substrate 1 and the second substrate 2 are made of plexiglass, respectively equipped with four micro-channels for forming the four micro-channels through the mutual cooperation. The first flow channel wall 11 and the second flow channel wall 21 of the flow channel, the first flow channel wall and the second flow channel wall have a transparent silica airgel layer, wherein each micro flow channel includes a microfluidic Valve section A, detection section B, discharge section C ( figure 1 ). The detection section B (500μm high) is composed of three sections of gradually narrowing flow channels, and the widest section is...

Embodiment 2

[0062] image 3 middle a is figure 2 The first substrate 1 and the second substrate 2 installed with the quartz drain tube 3 are combined to form a multifunctional cell counting imaging sample stage device by a thin layer of glass glue or double-sided adhesive. Wherein circled knife-type microfluidic valve 14 is open state, in image 3 The knife-type microfluidic valve 14 shown in b is in the closed state, and the sunken structure of the discharge section is circled, and the level of the sunken structure installed with the drain pipe 3 is shown. segment.

[0063] Such as image 3 As shown in c, on the four first optical interfaces 15 and the four second optical interfaces 25 corresponding to one of them, a threaded detachable plug 6 (that is, a bolt) is used to cooperate with the sealing ring to connect with the optical interface. The inner thread coupling screw-in method seals the widest section of the detection section B of the micro-channel to keep the valve in a close...

Embodiment 3

[0069] This embodiment is different from Embodiment 2 in that the first end of the first transparent cover 16 and the second transparent cover 26 are not installed, but the first ends of the two optical fiber light transmission accessories 9 are used to seal and connect the first optical interface 15 and the second optical interface respectively. 25 (sealed in the same way as the detachable blocking member 6, and sealed with a sealing material). Such as image 3 The illuminating device 7 and the detector 8 shown in middle g are respectively connected to the second ends of the respective optical fiber light-conducting fittings 9, so that the illumination light emitted by the illuminating device 7 passes through the optical fiber light-conducting fitting 9 and enters the inside of the micro-flow channel, while the transmitted light The second end of the optical fiber light transmission fitting 9 that is sealed and connected through the second optical interface 25 is imaged or re...

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Abstract

The invention provides a sample-residue-free heat-insulating reusable multifunctional cell counting and imaging device which comprises a first substrate and a second substrate which can be matched with each other to form at least one micro-channel, wherein the first substrate and the second substrate are respectively provided with a first flow channel wall and a second flow channel wall which are matched with each other to form the at least one micro flow channel, the first flow channel wall and the second flow channel wall being respectively provided with a super-hydrophobic / hydrophobic thermal insulation material layer or formed by super-hydrophobic / hydrophobic thermal insulation materials, or the hydrophobic thermal insulation material is formed in the first substrate material and the second substrate material around the first flow channel wall and the second flow channel wall. Heat insulation detection and residue-free export of the sample are realized, so that the optical detection device is convenient to position under multiple visual angles; when an inlet heating device and an infrared temperature measurement interface are introduced into the device, the temperature control function of the reagent and the sample can be realized; and convenient material setting and specific reaction conditions are provided for various micro-channel biochemical experiment requirements.

Description

technical field [0001] The invention relates to a cell counting imaging device, in particular to a reusable multifunctional cell counting imaging sample stage device without sample residue and heat insulation, which belongs to the field of micro flow channel devices. Background technique [0002] At present, most image cell counters on the market use disposable plastic counting plates, which are costly and disposable, and produce a large amount of white plastic waste that is difficult to digest, which brings great damage to the environment and does not meet the national carbon neutral requirements. The high cost of disposable consumables also wastes a lot of scientific research funds in the laboratory. The reusable cell counting imaging sample platform of the present invention can be reused without limitation, avoids white plastic pollution, and can also save nearly 100 million yuan in consumables costs every year. [0003] Considering the interaction between the inner wall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N15/10
CPCB01L3/5027G01N2015/1006G01N15/1023G01N15/01G01N2015/1024
Inventor 蔡应辉
Owner 高分(北京)生物科技有限公司
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