Breast cancer diagnosis marker and application thereof

A breast cancer, biological sample technology, applied in the field of breast cancer diagnostic markers, can solve problems such as high breast cancer risk, and achieve the effect of high accuracy

Pending Publication Date: 2022-02-11
HUZHOU CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germline mutations in TP53 may lead to higher breast cancer risk

Method used

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  • Breast cancer diagnosis marker and application thereof
  • Breast cancer diagnosis marker and application thereof
  • Breast cancer diagnosis marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Determination of the expression level of ADRM1 protein in breast cancer tissue

[0026] (1) Sample collection

[0027] A total of 148 surgically resected tissue samples from breast cancer patients diagnosed by pathology in our hospital were collected. The selection criteria for patients were: breast cancer confirmed by surgical resection and pathology; no radiotherapy, chemotherapy, hormone and biological targeted therapy before operation.

[0028] (2) Immunohistochemical detection

[0029] The breast cancer nest tissue paraffin specimens were sliced ​​continuously at 4 μm, routinely deparaffinized, washed with PBS, and operated according to the instructions of the immunohistochemical kit. The dilution of mouse anti-human ADRM1 was 1:500, and the dilution of mouse anti-human TP53 was 1: 100, the primary antibody was replaced with phosphate buffered saline (PBS) as a negative control. Color was developed with DAB at room temperature for 5-10 minutes, rinsed w...

Embodiment 2

[0040] Differences in the expression of ADRM1 and TP53 genes in breast cancer tissue and normal tissue in embodiment 2

[0041] The UALCAN database (http: / / ualcan.path.uab.edu / index.html) was used to analyze the expression level of ADRM1 in breast cancer. The screening conditions are as follows: gene symbol=ADRM1; TCGA dataset=Breast invasive carcinoma. There were 1097 breast cancer samples and 114 normal tissue samples that met the conditions. The UALCAN database was used to further analyze the relationship between ADRM1 and age, race, lymph node metastasis stage, histological grade and breast cancer classification, as well as its expression in mutant and non-mutant TP53 breast cancer samples. The results are shown in figure 1 .

[0042] Compared with the normal control group, the expression level of ADRM1 was increased in breast cancer patients (pfigure 1 A), and the expression level of ADRM1 was significantly correlated with age, race, lymph node metastasis stage, histolo...

Embodiment 3

[0044] The screening method of the miRNA of embodiment 3 ADRM1 and TP53

[0045] (1) Screening miRNAs that act together on ADRM1 and TP53

[0046] Through online databases miRTarBase (http: / / www.mirbase.org / ), Mirtarbase (http: / / mirtarbase.cuhk.edu.cn / php / index.php), TargetScan (http: / / www.targetscan.org / mamm_31 / ) and TransmiR (http: / / www.cuilab.cn / transmir) predicted the interaction relationship between ADRM1 and TP53 and miRNA. Cytoscape software (www.cytoscape.org / ) was used to construct and visualize the regulatory network of miRNAs on ADRM1 and TP53. It was found that, including hsa-let-7b-5p, hsa-miR-125b-5p, hsa-miR-24-3p, hsa-miR-1233-5p, hsa-miR-5693, hsa-miR-149-3p, hsa-miR-6766-5p, hsa-miR-4728-5p, hsa-miR-6756-5p, hsa-miR-19b-3, hsa-miR-6778-5p, hsa-miR-194-3p, hsa- 15 miRNAs including miR-6785-5p, hsa-miR-19a-3p and hsa-miR-6883-5 jointly target ADRM1 and TP53 ( image 3 ).

[0047] (2) Further screening of miRNAs that act together on ADRM1 and TP53

[0048...

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Abstract

The invention discloses a breast cancer diagnosis marker and application thereof. Expression of ADRM1 protein in breast cancer tissues is detected through an immunohistochemical method, and the result shows that ADRM1 is remarkably up-regulated in the cancer tissues of breast cancer patients and is remarkably related to the tumor size. Meanwhile, through UALCAN database analysis, it is found that the expression level of ADRM1 in TP53 mutant and non-mutant breast cancer is significantly higher than that in normal breast cancer, it is indicated that the expression of ADRM1 is closely related to the expression of TP53 in breast cancer, the expression of ADRM1 and TP53 protein in breast cancer patient tissue can be jointly detected through ADRM1 protein and TP53 protein, and the accuracy rate is high.

Description

technical field [0001] The invention relates to the field of tumor diagnosis, in particular to a breast cancer diagnostic marker and its application. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. Global cancer epidemiological statistics show that in 2012, there were about 1.7 million new cases of breast cancer in the world, and about 522,000 deaths. In China, breast cancer has become the first among women's high-incidence tumors, and the morbidity and mortality of breast cancer in the United States and other Western countries account for the second among all tumors. With the continuous improvement of contemporary medical treatment level, the cure rate and survival rate of breast cancer have been significantly improved, but the metastasis and recurrence of breast cancer are still serious risk factors threatening breast cancer patients. The occurrence of metastasis and recurrence not only It will increase the difficulty of tr...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57415G01N33/57484G01N2333/4748G01N2333/4703
Inventor 陈静
Owner HUZHOU CENT HOSPITAL
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