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Method for simultaneously extracting polysaccharides and flavones from abelmoschus manihot flowers

A technology of hollyhock flowers and polysaccharides, which is applied in the field of extraction of traditional Chinese medicine components, can solve the problems of cumbersome operation, large amount of organic reagents used, and long operating hours, and achieve the effect of simple operation, high purity, and rapid effect

Active Publication Date: 2022-01-21
SHENYANG RES INST OF CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above methods can separate polysaccharides and flavonoids from marshmallow flowers, but the operation is cumbersome and takes a long time, or one of the components is separated and prepared, and the amount of organic reagents used Large, the purity of the isolated polysaccharides and flavonoids is not high enough to meet market demand, and an easy-to-operate, rapid separation and preparation method is needed

Method used

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  • Method for simultaneously extracting polysaccharides and flavones from abelmoschus manihot flowers
  • Method for simultaneously extracting polysaccharides and flavones from abelmoschus manihot flowers
  • Method for simultaneously extracting polysaccharides and flavones from abelmoschus manihot flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Selection of Macroporous Resin for Decolorization of Polysaccharides from Melhock Flowers

[0023] Take 250g of hollyhock flowers (origin: Heilongjiang), add 2.5L of 55% ethanol at a material-to-liquid ratio of 1:10, heat and reflux for extraction for 2 hours, extract twice in this way, filter and combine the filtrate, and distill the filtrate to 0.3L under reduced pressure, add Adjust the concentration of ethanol in the concentrate to 75% with 95% ethanol, place it in a refrigerator at 4°C for 24 hours, collect the precipitated crude polysaccharide, and dry it in a vacuum oven to obtain 60.86 g of crude polysaccharide. The content of polysaccharide determined by sulfuric acid-phenol colorimetry is 70.69%. .

[0024] Take 55g of the above-mentioned crude polysaccharide and dissolve it in an appropriate amount of water, add Sevage reagent (chloroform / n-butanol 4:1) to mix according to 1 / 4 of the volume of the polysaccharide aqueous solution, shake it fully, let it stand ...

Embodiment 2

[0029] Selection of macroporous resin for decolorizing flavonoids from marshmallow flower

[0030] According to the description in Example 1, the crude polysaccharides were removed by extracting marshmallow flowers with ethanol, and the collected filtrate was concentrated by distillation under reduced pressure and dried in vacuum to obtain crude flavonoid samples. The above steps were repeated twice, and a total of 34.42 g of crude flavonoid samples were collected, with a flavonoid content of 32.12%.

[0031] The above-mentioned crude flavonoid sample is equally divided into 5 equal parts, and after every part is dissolved in 150mL of 20% ethanol, it is divided into 5 batches and adopts AB-8 macroporous resin adsorption (column volume 180mL), first rinsed with pure water to remove impurities, and then Use 65% ethanol to elute until colorless, collect the eluate, and concentrate each fraction to 100 mL by distillation under reduced pressure.

[0032] Add the above 5 parts of c...

Embodiment 3

[0036] (1) Preparation of polysaccharide from hollyhock flower

[0037] Take 100g of hollyhock flowers (origin: Heilongjiang), add 1L of 55% ethanol at a material-to-liquid ratio of 1:10, heat and reflux for extraction for 2 hours, extract twice in this way, filter and combine the filtrate, distill the filtrate to 0.1L under reduced pressure, add 95% Adjust the concentration of ethanol in the system to 75% with ethanol, place it in a refrigerator at 4°C for 24 hours, collect the precipitated crude polysaccharide, and dry it in a vacuum oven to obtain 24.33 g of crude polysaccharide. The polysaccharide content was determined to be 71.53% by sulfuric acid-phenol colorimetry.

[0038]Take 10g of the above-mentioned crude polysaccharide and dissolve it in an appropriate amount of water, add Sevage reagent (chloroform / n-butanol 4:1) to mix according to 1 / 4 of the volume of the polysaccharide aqueous solution, shake it fully, let it stand still, and centrifuge (5000r / min, 10min) to r...

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Abstract

The invention belongs to the field of extraction of traditional Chinese medicine components and particularly relates to a method for simultaneously extracting polysaccharides and flavones from abelmoschus manihot flowers. The method comprises the following steps: subjecting the abelmoschus manihot flowers to heated reflux extraction through an ethanol / water system, carrying out alcohol-precipitation separation and precipitation to obtain crude polysaccharides, concentrating supernatant, carrying out vacuum drying to obtain crude flavones, separately eluting and separating the separated polysaccharides and flavones through adsorption macroporous resin, and carrying out decoloration and impurity removal on the polysaccharides and the flavones through decoloration resin to separately prepare the high-purity abelmoschus manihot flower polysaccharides and flavones. The method has the advantages that the operation is simple, the impurity removal and decoloration effects are rapid, and the purity of the prepared abelmoschus manihot polysaccharides and flavones is high.

Description

technical field [0001] The invention belongs to the field of extraction of traditional Chinese medicine components, and in particular relates to a method for simultaneously extracting polysaccharides and flavonoids from marshmallow flowers. Background technique [0002] Hollyhock is an annual or perennial plant of the genus Okra in the Malvaceae family. It is also known as okra, cotton sunflower, wild hibiscus, and chicken claw lotus. It is widely distributed in India, Nepal and China. In my country, it is mainly distributed in Yunnan, Guizhou, Sichuan, Hubei, Guangdong, Guangxi and Taiwan provinces and regions; hollyhock flower corolla is a traditional Chinese medicinal material, which is usually used for many different purposes such as herbal medicine, tea drinking and health care. It has high medical and health care value, such as beauty and beauty, Increase appetite, assist in the treatment of kidney disease, treat inflammation, etc. [0003] Hollyhock flowers are rich in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185C08B37/00A61K133/00
CPCA61K36/185C08B37/0003A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 王远王加友李欣孙丽娟杨德玉焦姣李小明于丹萌
Owner SHENYANG RES INST OF CHEM IND
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