Novel utensil for zebra fish behavioral experiment

Abstract

The invention relates to a novel utensil for a zebra fish behavioral experiment. The novel utensil comprises a cavity body, wherein the cavity body comprises an experimental target observation area and an experimental environment configuration area; the experimental target observation area is arranged in the center of the cavity body, and the experimental environment configuration area surrounds the experimental target observation area; the experimental environment configuration area comprises a baffle and a spacer; the baffle longitudinally divides the experimental environment configuration area, and the spacer is detachably connected with the baffle; and information for stimulation is carried on the spacer. The novel utensil for the zebra fish behavioral experiment has the advantages that the experimental environment is flexible and changeable, specialized experiment requirements for zebra fish can be met, experiment means can be supplemented, and the experiment range can be expanded.

Description

technical field [0001] The invention relates to the technical field of experimental equipment, in particular to a novel vessel for zebrafish behavior experiments. Background technique [0002] With the development of model animal zebrafish experiments and people's attention to brain science, the degree of attention to the learning, memory and complex behavior of organisms is gradually increasing worldwide. The existing zebrafish behavior related experiments are mainly T maze and cross maze. , these utensils can be customized according to different experimental purposes to meet experimental needs including color tendency, reward and punishment memory, etc., but they focus on adult zebrafish, and insufficient attention is paid to juvenile fish, and early life is important for intellectual development and environmental exposure. The window period is crucial. [0003] The currently used behavioral maze has a single exposure factor and needs to be customized repeatedly according...

AI Technical Summary

Problems solved by technology

[0003]The behavioral maze currently used has a single exposure factor, which needs to be customized repeatedly according to the purpose of the experiment. At the same time, a certain 'non-exposure' area is defined. In fact, in these areas Animals are also affected by exposure factors, and there are brain activities and choices. However, how to define this range is very difficult. At the same time, the lack of compound environmental exposures limits the research on animal environmental compound exposures and complex movements.

[0004] Chinese patent document CN110558247A discloses a combined multi-arm maze device, comprising a partition arm and a combined step, the partition arm is composed of two side plates and a sealing plate One-way channel structure is formed, one end of the two side plates is sealed by a sealing plate, and the other end is open. There are several partition arms, and the opening ends of several partition arms are connected in sequence in the circumferential direction, and are enclosed in the middle to form a central empty area. A combined step is arranged in the one-way channel of each dividing arm; it can be used for the experimental research of animal movement behavior control, and can be used for the experimental research of animal learning and memory ability; The assembly of the divider arm in different directions, and the establishment of the combined steps in it, provide different movement direction selection barriers, and set up space barriers on the way to conduct experimental research on terrestrial and aquatic animals, obviously for ethology In the experiment, a baffle can be temporarily placed between the central empty area and each dividing arm, and the temporary closing and opening of the channel can be realized, the purpose of which is to train the memory ability of the rat; for the zebrafish behavior experiment to be solved in this application The equipment cannot be used universally, and the problem of flexibility cannot be solved. For example, the shape of the central space is determined by the number of partition arms. In the experiment of optokinetic response, the experiment of the motion effect of the grating cannot be realized, etc.

As above, this technical solution integrates four kinds of mazes on one device, which does not form a breakthrough in the experimental procedures. Compared with traditional experiments, it only lies in the fact that fewer experimental devices can be used for assembly

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