Preparation method of flexible biosensor electrode

A biosensor, electrode technology, applied in the direction of instruments, scientific instruments, material electrochemical variables, etc., can solve the problems of physiological pain and psychological pressure of patients, and achieve the effect of low price, simple process and good tensile properties

Pending Publication Date: 2022-01-14
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, rigid glassy carbon electrodes or commercial blood glucose meters were mostly used to monitor the glucose content in the blood. Such methods are invasive and will cause certain physical pain and psychological pressure to patients.

Method used

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  • Preparation method of flexible biosensor electrode
  • Preparation method of flexible biosensor electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of chemical gold plating solution:

[0027] Mix the potassium bicarbonate aqueous solution and the glucose solution uniformly at a volume ratio of 1:1:2, and finally add an aqueous chloroauric acid solution to form an electroless gold plating solution.

[0028] The potassium bicarbonate aqueous solution concentration used for mixing above is 200g / L, the glucose solution concentration is 0.1M, and the chloroauric acid aqueous solution concentration is 1 wt.%.

[0029] 2. Preparation of dopamine hydrochloride solution:

[0030] Tris-HCl buffer solution with a concentration of 10 mM and a pH of 8.0 was used to prepare a dopamine hydrochloride solution with a concentration of 1 mg / mL.

[0031] 3. Preparation of polydimethylsiloxane:

[0032] The polydimethylsiloxane prepolymer and curing agent (both purchased from Dow Corning, USA) were mixed at a mass ratio of 10:1, and a crosslinking reaction occurred to form polydimethylsiloxane.

[0033] 4. Preparation...

Embodiment 2

[0049] Referring to the preparation method of Example 1, the difference is that in the preparation of the PDMS film, the mixture of the polydimethylsiloxane prepolymer and the curing agent is degassed.

[0050] The specific operation is as follows: when the polydimethylsiloxane prepolymer and the curing agent are mixed until many dense small bubbles appear, the mixture is placed in a vacuum oven for degassing treatment. Then the degassed mixture was evenly spin-coated onto a glass slide by a spin coater, and finally cured in an oven at 60° C. for 2 hours. No small air bubbles are generated in the fully cured PDMS film prepared by the method, which can avoid the influence on the deposition of subsequent gold nanoparticles.

Embodiment 3

[0052] Referring to the preparation method of Example 1, the difference is that in the preparation of the working electrode, the concentration of Ni / Co-MOF in the Ni / Co-MOF dispersion is adjusted.

[0053] The details are as follows: 1 mg, 2 mg, 3 mg, 4 mg, and 5 mg of Ni / Co-MOF powder were dissolved in 100 μL of 1% Nafion ethanol solution, and ultrasonicated for 5 minutes at room temperature until different Ni / Co-MOFs were formed. concentrated dispersion.

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Abstract

The invetion relates to a preparation method of a flexible biosensor electrode and relates to the technical field of electrochemical sensors, and the methid comprises the following steps: by taking a PDMS film as a substrate, carrying out surface modification on the substrate, and then preparing a patterned three-electrode by using a chemical gold deposition method; and constructing a flexible biosensor for glucose detection by fixing a Ni / Co-MOF nano material with electrocatalytic activity on a working electrode. Complex equipment is not needed, the price is low, and the constructed sensor can be applied to real-time monitoring of human body disease signals.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to a preparation method of non-enzyme flexible biosensor electrodes. Background technique [0002] Glucose is an important substance in living organisms, and its content in body fluids is an important indicator reflecting the state of the body. A large number of glucose sensors have been developed. Previously, rigid glassy carbon electrodes or commercial blood glucose meters were mostly used to monitor the glucose content in the blood. Such methods are invasive and will cause certain physical pain and psychological pressure to patients. Contents of the invention [0003] In order to overcome the defects of the prior art, the object of the present invention is to provide a method for preparing a flexible biosensor electrode capable of real-time monitoring of glucose. [0004] The present invention comprises the following steps: [0005] 1) Mix polydimethylsiloxan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30G01N27/327
CPCG01N27/48G01N27/301G01N27/3278
Inventor 舒韵尚振娇胡效亚
Owner YANGZHOU UNIV
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