Stem cell composition applied to biological tissue repair and preparation method thereof

A technology of biological tissue and stem cells, applied in prosthetics, medical science, etc., can solve the problems of insufficient simplification of the preparation process, increase of manufacturing costs, stimulation, etc., and achieve the effect of streamlining and efficient preparation methods, avoiding secondary damage, and improving tissue repair

Inactive Publication Date: 2022-01-11
广东龄值生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current application of stem cells in the repair of biological tissues usually requires the use of autologous stem cells for cultivation, which will cause certain damage to the body, which takes a lot of effort and time, is not friendly to the organism itself, and is not convenient enough
[0004] In addition, at present, the directional induction of hematopoietic stem cells and nerve cells has been relatively mature, but the use of other stem cells, especially adipose-derived stem cells, to induce tissue repair is relatively immature, and there are certain limitations in the use of directional induced stem cells for biological tissue repair. Difficulty and pitfalls
The main problems are, first, it is difficult to accurately perform directional induction and quickly repair tissues; second, some toxic or tissue-irritating substances need to be added during the induction process, such as 2-propylvaleric acid and β-mercaptoethanol etc. It is easy to cause irritation in the process of tissue repair and produce certain toxic and side effects, which is not safe enough; thirdly, when using stem cells for tissue repair, although some techniques can heal difficult-to-heal wounds, they leave obvious scars 4. The composition is complicated, the preparation process is not streamlined enough, it is easy to cause allergies, and it also increases the manufacturing cost

Method used

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  • Stem cell composition applied to biological tissue repair and preparation method thereof
  • Stem cell composition applied to biological tissue repair and preparation method thereof
  • Stem cell composition applied to biological tissue repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: A stem cell composition applied to biological tissue repair, including adipose stem cells, adipose stem cell exosomes, epidermal stem cell exosomes, axolotl and collagen short peptides, and a tissue repair carrier.

[0031] Wherein, the mass ratio between salamandertin and collagen short peptide is 1:100; the collagen short peptide is a mixture of type I collagen and marine collagen short peptide, and is mixed at a mass ratio of 4:1.

Embodiment 2

[0032] Example 2: A stem cell composition applied to biological tissue repair, including fat stem cells, fat stem cell exosomes, epidermal stem cell exosomes, axolotl and short collagen peptides, as well as acetaminophen and gene cell growth peptide ; Also includes tissue repair vectors.

[0033] Wherein, the mass ratio between salamandertin and collagen short peptide is 1:30; the collagen short peptide is a mixture of type I collagen and marine collagen short peptide, and is mixed at a mass ratio of 2:1.

Embodiment 3

[0034] Example 3: A stem cell composition applied to biological tissue repair, including fat stem cells, fat stem cell exosomes, epidermal stem cell exosomes, axolotl and collagen short peptides, as well as acetaminophen and gene cell growth peptide ; Also includes tissue repair vectors.

[0035] Wherein, the mass ratio between salamandertin and collagen short peptide is 1:80; the collagen short peptide is a mixture of type I collagen and marine collagen short peptide, and is mixed at a mass ratio of 3:1; The molecular weight of the short collagen peptide is less than 300 Daltons.

[0036] Above-mentioned embodiment 1-3, all make by following preparation method, comprise the following steps:

[0037] (1) Preparing adipose stem cells: obtaining animal adipose tissue, and filtering the adipose tissue through a sieve to obtain adipose stem cells;

[0038](2) Obtaining adipose stem cell exosomes: After ultra-low temperature freezing of the adipose stem cell suspension obtained i...

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Abstract

The invention discloses a stem cell composition applied to biological tissue repair and a preparation method thereof, and belongs to the technical field of biology, the stem cell composition comprises adipose-derived stem cells, adipose-derived stem cell exosomes, epidermal stem cell exosomes, samandatrin and collagen oligopeptide; the method comprises the following steps: (1) preparing adipose-derived stem cells; (2) obtaining adipose-derived stem cell exosomes; (3) obtaining epidermal stem cell exosomes; and (4) fully mixing; and obtaining a finished product. According to the stem cell composition, biological tissue repair can be carried out without autologous stem cells, the components are simple, obvious scars cannot appear after repair, the activity of the stem cells can be kept through the strictly designed preparation method, meanwhile, stable proceeding of subsequent differentiation of the stem cells is guaranteed, and tissue repair is rapidly carried out.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a stem cell composition applied to biological tissue repair and a preparation method thereof. Background technique [0002] With the development of modern science and technology, the application of stem cells in biological tissues has become more and more in-depth. Clinical trials have shown that stem cells can be used to repair the wounds of living organisms, especially for the repair of some difficult-to-heal wounds. brings hope. [0003] However, the current application of stem cells in the repair of biological tissues usually requires the use of autologous stem cells for cultivation, which will cause some damage to the body, which is time-consuming, not friendly to the organism itself, and not convenient enough. [0004] In addition, at present, the directional induction of hematopoietic stem cells and nerve cells has been relatively mature, but the use of other stem cells, espe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24A61L27/36A61L27/54
CPCA61L27/3834A61L27/24A61L27/3604A61L27/54A61L2300/30A61L2300/252A61L2300/412C08L89/00
Inventor 马颖马铎
Owner 广东龄值生物科技有限公司
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