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Method for establishing EAM mouse model induced by autoantigen epitope

A technology of mouse model and method establishment, which is applied in the fields of radiological diagnostic equipment, image data processing, diagnosis, etc., can solve the problems of prone to individual differences, difficult to fully grasp the stability of indicators, and large fluctuations of indicators, etc., to achieve Moderate molding time, low price, and high model stability

Active Publication Date: 2022-01-11
天津医科大学总医院空港医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method can reflect the level of myocardial inflammation and fibrosis to a certain extent, these indicators often fluctuate greatly in the body, which is prone to individual differences, and the stability of indicators is difficult to fully grasp.

Method used

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  • Method for establishing EAM mouse model induced by autoantigen epitope
  • Method for establishing EAM mouse model induced by autoantigen epitope
  • Method for establishing EAM mouse model induced by autoantigen epitope

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Embodiment Construction

[0047] Embodiments of the present application will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present application, rather than limiting the scope of the present application. Various objects and advantages of the present application will become apparent to those skilled in the art from the following detailed description of the preferred embodiments.

[0048] In order to further understand the present invention, the embodiment of the present invention will be described in detail below in conjunction with the examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested...

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Abstract

The invention provides a method for establishing an EAM (Experimental Autoimmune Myocarditis) mouse model induced by an autoantigen epitope. According to the method, the relationship between AchR and the EAM is determined for the first time, an AchR-Freund's complete adjuvant solution, an AchR-Freund's incomplete adjuvant solution and an immune auxiliary enhancer are injected into a mouse for modeling, an antibody aiming at a myocardial cell M2 cholinergic receptor is generated to induce the EAM, and the established model has the characteristics of myocardial cell hypertrophy, myocardial fibrosis, cardiac dysfunction and the like. Meanwhile, 18F-FDG PET / CT myocardial metabolism imaging is taken as a core, and means of cardiac ultrasound, myocardial tissue slice HE staining, myocardial Mason staining, myocardial tissue immune antibody specific staining and the like are combined to verify characteristic changes related to the mouse model. The new mouse model is provided for further research on the EAM.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an EAM mouse model induced by an autoantigen epitope. Background technique [0002] Myocarditis is an inflammatory disease characterized by myocardial inflammatory cell infiltration and non-ischemic necrosis of myocardial cells. The clinical manifestations of myocarditis vary greatly. Mild cases have no obvious clinical symptoms and are easily overlooked. In severe cases, the myocardium is damaged, leading to arrhythmia, cardiogenic shock or sudden death. The etiology of myocarditis can be divided into infectious factors, such as viruses, bacteria, parasites, chlamydia, fungi, protozoa, etc., and non-infectious factors, such as autoimmune diseases, chemical and drug toxins and allergic reactions. [0003] In the past 2 years, autoimmune myocarditis has received high attention due to the use of PD-1 / PD-L1 monoclonal antibody to treat tumors and t...

Claims

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Application Information

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IPC IPC(8): A01K67/027G16B15/30G06T7/64G06T7/00A61B6/03A61B6/00G01N33/68
CPCA01K67/027G01N33/6854G16B15/30G06T7/0012G06T7/64A61B6/508A61B6/505A61B6/032A61B6/037A61B6/5217A01K2207/12A01K2227/105A01K2267/0375A01K2207/10A01K2267/0325G06T2207/10081G06T2207/30012
Inventor 张鹏杨照宇王元国陈思宋世辉王欢
Owner 天津医科大学总医院空港医院
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