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Biological indicator for monitoring sterilization effect and preparation method thereof

A biological indicator, fructose technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as long detection time and reduced sterilization process efficiency.

Pending Publication Date: 2021-12-31
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently widely used biological indicators have a long detection time, which reduces the efficiency of the industry involved in the sterilization process

Method used

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  • Biological indicator for monitoring sterilization effect and preparation method thereof
  • Biological indicator for monitoring sterilization effect and preparation method thereof
  • Biological indicator for monitoring sterilization effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of the medium for germination and growth of spores of the present invention

[0036] Medium formula: tryptone 10g, brain heart infusion broth medium 10g, fructose 3g, glucose 2g, potassium chloride 1g, dipotassium hydrogen phosphate 1g, Tween 80 1g, L-alanine 5g, L-lysine Amino acid 1g, L-valine 1g, 2,6-pyridinedicarboxylic acid 2.1g, CaCl 2 1.4g, manganese chloride 10mg, 4-methylumbelliferyl-α-D-glucopyranoside (4-MUG) 200mg, bromine methyl violet 20mg, distilled water to make up to 1000mL, adjust its pH to 7.4-7.8 .

[0037] Preparation

[0038] 1) Weigh 200 mg of 4-MUG according to the proportion, dissolve it with 0.5 mL of dimethyl sulfoxide to obtain a 4-MUG solution; weigh L-alanine, L-lysine and L-valine according to the proportion, add 50 mL of water Dissolve to obtain amino acid solution;

[0039] 2) Take the remaining raw materials, add 950 mL of distilled water to dissolve, and sterilize at 121° C. for 20 minutes to obtain a culture...

Embodiment 2

[0041] Example 2 Preparation of the medium for germination and growth of spores of the present invention

[0042] Medium formula: tryptone 5g, brain heart extract broth 5g, fructose 2g, glucose 2g, potassium chloride 0.5g, dipotassium hydrogen phosphate 0.5g, Tween 800.5g, L-alanine 3g, L-lysine Amino acid 0.5g, L-valine 1g, 2,6-pyridinedicarboxylic acid 1.7g, CaCl 2 1.0g, manganese chloride 5mg, 4-methylumbelliferoyl-α-D-glucopyranoside (4-MUG) 100mg, bromine methyl violet 10mg, distilled water to make up to 1000mL, adjust its pH to 7.4-7.8 .

[0043] Preparation

[0044] 1) Weigh 4-MUG according to the proportion, dissolve it with 0.5mL dimethyl sulfoxide to obtain 4-MUG solution; weigh L-alanine, L-lysine and L-valine according to the proportion, add 50mL water Dissolve to obtain amino acid solution;

[0045] 2) Take the remaining raw materials, add 950 mL of distilled water to dissolve, and sterilize at 121° C. for 20 minutes to obtain a culture medium mother liquor. ...

Embodiment 3

[0047] Example 3 Preparation of the medium for germination and growth of spores of the present invention

[0048] Medium formula: tryptone 15g, brain heart infusion broth 15g, fructose 4g, glucose 3g, potassium chloride 1.5g, dipotassium hydrogen phosphate 1g, Tween 80 2g, L-alanine 7g, L-lysine Acid 1.5g, L-valine 1.5g, 2,6-pyridinedicarboxylic acid 2.5g, CaCl 21.8g, manganese chloride 15mg, 4-methylumbelliferyl-α-D-glucopyranoside (4-MUG) 300mg, bromomethyl violet 30mg, distilled water to make up to 1000mL, adjust its pH to 7.4-7.8 .

[0049] Preparation

[0050] 1) Weigh 4-MUG according to the proportion, dissolve it with 0.5mL dimethyl sulfoxide to obtain 4-MUG solution; weigh L-alanine, L-lysine and L-valine according to the proportion, add 50mL water Dissolve to obtain amino acid solution;

[0051] 2) Take the remaining raw materials, add 950 mL of distilled water to dissolve, and sterilize at 121° C. for 20 minutes to obtain a culture medium mother liquor.

[0052]...

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Abstract

The invention discloses a culture medium for spore germination and growth, a biological indicator prepared by using the culture medium and corresponding preparation methods of the culture medium and the biological indicator. Through the specific culture medium, the spore germination and acid production time is shorter, the response value detected by a biological indicator reader is higher, the response time is shorter, the spore germination detection result is faster and more accurate than that of mature biological agents in the current market on the whole, and thus, the application and popularization value is achieved.

Description

Technical field [0001] The invention specifically relates to a biological indicator used for monitoring sterilization effects and a preparation method thereof. Background technique [0002] Sterilization refers to measures that use strong physical and chemical factors to cause all microorganisms inside and outside any object to permanently lose their ability to grow and reproduce. Commonly used methods of sterilization include chemical reagent sterilization, radiation sterilization, dry heat sterilization, moist heat sterilization and filter sterilization. The thoroughness of sterilization is affected by the resistance of microorganisms to the sterilization method, the sterilization time and the sterilization time. Constraints on mode strength. Since sterilization is a necessary condition for obtaining pure culture, sterilization technology is now used in many industries, such as the food industry, medicine, and agricultural cultivation. The effect of sterilization is direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/22C12Q1/04C12R1/07
CPCC12N1/20C12Q1/22C12Q1/045Y02A50/30
Inventor 王丹巫明毫饶雨露
Owner CHENGDU MEDICAL COLLEGE
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