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Method for establishing radiation-induced depression rat animal model

A technology of animal model and establishment method, which is applied in animal husbandry and other fields, can solve the problems of high animal mortality, poor prognosis, uneven effect, etc., to avoid high animal mortality, easy operation and good reproducibility Effect

Pending Publication Date: 2021-12-24
CHINA INST FOR RADIATION PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for establishing a radiation-induced depression rat animal model, which can establish a depression rat animal model with short cycle, low cost, easy operation, good reproducibility, and significant depression effect, avoiding the traditional olfactory bulb The modeling process of enucleation surgery caused problems such as high animal mortality, poor prognosis, and uneven results

Method used

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  • Method for establishing radiation-induced depression rat animal model
  • Method for establishing radiation-induced depression rat animal model
  • Method for establishing radiation-induced depression rat animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Establishment of an animal model of radiation-induced depression in rats

[0018] (1) Instruments and equipment

[0019] Rat brain stereotaxic instrument (stoelting 51621), linear accelerator Elekta Synergy (UK Elekta Limited company).

[0020] (2) Animals and groups

[0021] 24 healthy male SPF grade SD rats were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (production license number: SCXK (Beijing) 2016-0006), weighing 180-200g. According to the weight of the animals, they were randomly divided into control group and model group, with 12 animals in each group. Rats were reared in separate cages, 5 in each cage, free to eat and drink, and the feed was SPF large and mouse maintenance feed produced by Beijing Keaoxieli Feed Co., Ltd. Feed production license: Beijing Feeding Certificate (2018) 06073, Breeding Room The temperature is controlled at 20-25°C, and the humidity is controlled at 40-70%. Rat breeding cages and drink...

Embodiment 2

[0025] Embodiment 2: the detection of the establishment of rat animal model in embodiment 1

[0026] (1) Changes in animal weight

[0027] After radiation damage to the olfactory bulb of the rats, irritability and activity increased. The results in Table 1 show that the weight of the rats in the model group was lower than that of the control group on the 7th, 10th, and 14th day (P<0.05), indicating that the olfactory bulb damage has a certain impact on the weight gain of animals .

[0028] Table 1 Body weight changes of SD rats after olfactory bulb radiation modeling

[0029]

[0030] Note: *P<0.05 compared with the control group.

[0031] (2) Open field test

[0032] Self-made open field test chamber, 1×1×0.7m 3 Paint the inside of the box into black with black paint, and paste a white narrow tape on the bottom of the box to make it a small square of 20cm×20cm.

[0033] Open field test: put the rat in the center of the self-made open box, after 2 minutes of adaptation...

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Abstract

The invention belongs to the technical field of animal model construction, and relates to a method for establishing a radiation-induced depression rat animal model. According to the method, an anesthetized rat olfactory bulb is damaged by radiating the olfactory bulb, so that the depression rat animal model is established. The method for establishing the radiation-induced depression rat animal model can be used for establishing the depression rat animal model, which is short in period, low in cost, simple and convenient to operate, good in reproducibility and remarkable in depression effect, and the problems of high animal death rate, poor healing, uneven effects and the like caused by a traditional olfactory bulb removal surgery modeling process are avoided.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and relates to a method for establishing a radiation-induced depression rat animal model. Background technique [0002] There are many causes of depression, and there is still no more unified conclusion. The pathogenesis of depression mainly includes monoamine transmitter hypothesis, receptor hypothesis, axis hypothesis and neuroplasticity hypothesis. The olfactory bulb is closely related to the above hypotheses. The olfactory bulb can participate in the pathological regulation of depression by affecting neuroplasticity, biological rhythm, endocrine, and the expression of monoamine transmitters and their receptors. The olfactory bulb plays an important role in vital functions, and its signal regulation is involved in the management of neural plasticity and olfactory function. [0003] Clinical data show that the sensitivity of smell and the volume of olfactory bulb are reduced...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/35A01K2227/105A01K2267/03
Inventor 刘欢秦秀军张伟黄立群刘梦雅许文黎尹晶晶王新钢王若琪李建国安全
Owner CHINA INST FOR RADIATION PROTECTION
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