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Application of octenidine dihydrochloride to preparation of antitumor drug

A technology of octenidine dihydrochloride and antitumor drug, which is applied in the application field of octenidine dihydrochloride in the preparation of antitumor drugs, can solve the problems of stimulating phagocytosis and growth factors, and achieves inhibition of Effects of growth and proliferation

Pending Publication Date: 2021-12-21
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

OCT also stimulates phagocytosis and growth factors, which are beneficial to wound healing [4] , but the role of OCT in tumor cells has not been reported

Method used

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  • Application of octenidine dihydrochloride to preparation of antitumor drug
  • Application of octenidine dihydrochloride to preparation of antitumor drug
  • Application of octenidine dihydrochloride to preparation of antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 in vitro experiment

[0029] (1) Detection of the effect of OCT on cell proliferation: Cells CNE-1 and SUNE-1 were placed in 96-well plates, 5000 cells were placed in each well, and cultured at 37°C, CO 2 In an incubator with a content of 5%, after 24h, it was treated with different concentrations of OCT (final concentrations were 0, 0.2, 0.4, 0.8, 1.6 and 3.2μM), and the treatment time was 24h. The CCK-8 cell proliferation detection kit ( Targetmol) to detect cell viability, and to study the effects of different concentrations of OCT on the proliferation of nasopharyngeal carcinoma cells.

[0030] (2) Detection of cell apoptosis by flow cytometry: before the detection by flow cytometry, the cells CNE-1 were treated with OCT, that is, the CNE-1 cells were cultured in a six-well plate, and the number of cells reached 24 hours per well after 24 hours. 70%, and then treated CNE-1 cells with different concentrations of OCT (final concentrations of 0, 0.4, 0.8,...

Embodiment 2

[0032] Embodiment 2 in vivo experiments

[0033] (1) Establishment of nasopharyngeal carcinoma mouse model: 6-week-old, NCG male mice (purchased from Nanjing Model Animal Research Center) were selected, and the nasopharyngeal carcinoma mouse model was constructed by subcutaneous injection, that is, cell suspension (nasopharyngeal carcinoma) Cancer cells CNE-1) were mixed with Matrigel (Corning Matrigel Matrix) at a volume ratio of 1:1, and each mouse was injected with 2x10 6 cells (use a 25G needle microsyringe to take the resuspended cells and inject them subcutaneously into mice). After subcutaneous injection, the tumor size reached 5x5mm for follow-up experiments.

[0034] (2) The experiment is divided into three groups: low-concentration drug treatment group (5 animals, injection volume is 3mg / kg); high-concentration drug treatment group (5 animals, injection volume is 6mg / kg); control group (5 animals, injection volume is 6mg / kg); conventional commercial corn oil equal ...

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Abstract

The invention discloses an application of octenidine dihydrochloride to preparation of an antitumor drug. In-vitro cell experiments show that OCT can inhibit growth and proliferation of tumor cells such as nasopharyngeal carcinoma cells; and mouse in-vivo experiments find that in-vivo subcutaneous tumor formation of a nude mouse is obviously inhibited by the OCT, it is indicated that the OCT can inhibit growth of nasopharyngeal carcinoma cells, and immunohistochemical detection proves that the OCT is safe to use as the antitumor drug. Therefore, the OCT can be used for preparing the antitumor drug for treating tumors such as nasopharyngeal carcinoma, esophageal squamous carcinoma, colorectal cancer or lung cancer, and a new drug source is provided for adjuvant therapy of cancers.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to the application of octenidine dihydrochloride in the preparation of antitumor drugs. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a regional malignancy with an incidence rate of 0.4 cases per 100,000 people in the United States, while most new cases occur in countries of Central and Southeast Asia. In addition, the incidence of nasopharyngeal carcinoma has a certain ethnic and family genetic aggregation, for example: Bidayu people in Borneo and Naga people in northern India [1] . Nasopharyngeal carcinoma is a malignant tumor that occurs in the outermost epithelial layer of the nasopharyngeal cavity, above the oropharynx and hypopharynx, near the base of the skull. [0003] Conventional treatments for NPC include radiotherapy or chemotherapy or a combination of both. In areas with a high incidence of nasopharyngeal carcinoma, limited radiotherapy techniques ...

Claims

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Application Information

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IPC IPC(8): A61K31/444A61P35/00
CPCA61K31/444A61P35/00
Inventor 汪洋张静欧阳兰何庆瑜余楠楠
Owner JINAN UNIVERSITY
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