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Application of physalis pubescens persistent calyx extract

A physalis, extract technology, applied in application, food ingredients containing natural extracts, medical preparations containing active ingredients, etc. Effect

Inactive Publication Date: 2021-12-03
JINZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research mainly focuses on the antitumor activity of withanolalide, and the correlation between a large number of chemical substances, especially flavonoids, and their efficacy is extremely limited. In research, there is little research on the efficacy of Physalis trichomes extract and its main chemical substances

Method used

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  • Application of physalis pubescens persistent calyx extract
  • Application of physalis pubescens persistent calyx extract
  • Application of physalis pubescens persistent calyx extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A preparation method of Physalis edulis extract, comprising:

[0053] (1) Ultrasonic-assisted water-bath oscillation (UAWBSE) crude extraction, with flavonoids as evaluation indicators.

[0054] Physalis trichomes with calyx fruit in Jinzhou Fruit Wholesale Market. The fruit is separated from the persistent calyx, and the persistent calyx is oven-dried at 50°C to constant weight, crushed into powder by a pulverizer, sealed and stored in a -80°C refrigerator for later use. Take 14g of Physalis trichomes powder, add ethanol concentration 74% according to the solid-liquid ratio of 80mL / g, extract in a 30W power ultrasonic machine for 15min, then shake in a water bath at 50rpm at 65°C for 3.4h, filter and concentrate to 10mL, It is required that the flavonoid content of Physalis trichomes should reach 8.71±0.11mg / g DW.

[0055] According to the influence of solid-liquid ratio, the influence of ethanol concentration, the influence of ultrasonic time, the influence of extra...

Embodiment 2

[0091] Screening and composition of the ethyl acetate EAFPC "efficacy group" prepared in Example 1

[0092] Equipment required for experiment

[0093] Agilent 1209Infinity LC system and 6540 accurate-mass quadrupole time-of-flight mass spectrometry system (Accurate-mass Quadrupole Time-of-Flight (Q-TOF) MS system);

[0094] Chromatographic column: Agilent Zorbax Eclipse Plus C18 chromatographic column (2.1×50mm, 1.8μm), which is a high-performance silica gel-based chromatographic column upgraded by Agilent through silica gel manufacturing technology and bonding technology. The 1.8μm chromatographic column has the characteristics of high resolution and excellent compound identification ability.

[0095] HPLC-Q-TOF-MS / MS detection conditions

[0096] Mobile phase: water with 0.1% formic acid (A) and chromatographic grade acetonitrile (B).

[0097] Gradient conditions: Take B as an example, initially 15%, rise to 40% after 25 minutes, then rise to 95% after 10 minutes, keep fo...

Embodiment 3E

[0103] Example 3 The inhibitory effect of EAFPC on liver cancer cells

[0104] online databases used

[0105] PubChem database (https: / / pubchem.ncbi.nlm.nih.gov / )

[0106] Targetswiss (http: / / www.swisstargetprediction.ch / )

[0107] STINCH (http: / / stitch.embl.de / )

[0108] TCGA database (https: / / cancergenome.nih.gov / )

[0109] TTD (http: / / database.idrb.cqu.edu.cn / TTD / )

[0110] DrugBank database (http: / / www.drugbank.ca / )

[0111] OMIM database (http: / / www.omim.org / )

[0112] GAD (http: / / geneticassociationdb.nih.gov / )

[0113] PharmGKB (https: / / www.pharmgkb.org / index.jsp)

[0114] (1) Screening of EAFPC targets

[0115] The targets of the 16 components of EAFPC were predicted according to the principle of structural similarity, and the two databases of targetswiss and STINCH were used for joint prediction. The component structure information is obtained from the PubChem database, and the SMILES entry of the component is recorded, and then the SMILES is input into the Ta...

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Abstract

The invention discloses a technical scheme for applying a physalis pubescens persistent calyx extract to preparation of a health care product for assisting in protecting the liver and preparation of a medicine for blocking the occurrence and development process of the liver cancer; and all the components in the extract have a synergistic effect, have a synergic regulation target spot and have a remarkable inhibition effect on liver cancer cells.

Description

technical field [0001] The invention relates to the technical fields of biomedicine and food, and more specifically relates to the application of the extract of Physalis pilaris persistent calyx. Background technique [0002] Physalis is rich in characteristic phytochemicals such as withanolide and a variety of significant physiological activities, so it has been widely concerned by the industries of medicine, food, cosmetics and so on. Among them, physalis is an annual herbaceous plant, also known as foreign girl, yellow mushroom mother, etc. It is a characteristic plant in Northeast China that has only attracted the attention of the pharmaceutical and food industries in recent years. Physalis edulis can not only be used as a good source of dietary nutrients, but also its anti-inflammatory, detoxification, anti-oxidation, anti-tumor and other health benefits are widely spread, but there is a lack of material basis research and modern scientific evidence. [0003] At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A23L33/105A61P35/00A61P1/16
CPCA61K36/81A61P35/00A61P1/16A23L33/105A23V2002/00A23V2200/308A23V2250/21
Inventor 王晶晶
Owner JINZHOU MEDICAL UNIV
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