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Insecticidal Seriphidium borotalense essential oil and application thereof

A technology of essential oils and insecticides, applied in the field of botanical pesticides, can solve the problems of unreported insecticidal activity, etc., and achieve the effects of easy to obtain reagents, high pest killing rate, and simple production methods

Active Publication Date: 2021-12-03
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the insecticidal activity of Bolota Seraphim in terms of plant-derived pesticides, especially the insecticidal activity of Bolota Seraphim

Method used

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  • Insecticidal Seriphidium borotalense essential oil and application thereof
  • Insecticidal Seriphidium borotalense essential oil and application thereof
  • Insecticidal Seriphidium borotalense essential oil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This example provides the preparation process and GC-MS test of the essential oil of bollotta serica.

[0016] The aerial part of the plant of Artemisia bolotta was naturally dried in the shade, crushed, weighed 500g, placed in a volatile oil extractor, and extracted by steam distillation, and the distillation time was 6h. The distillate was extracted with n-hexane to obtain the n-hexane layer, added anhydrous sodium sulfate to remove water, concentrated, measured the volume, put the volatile oil into a dry glass bottle, and stored it in a refrigerator at 4°C, which was the Boluo Tasmania essential oil.

[0017] The essential oil sample of Bolotta Serica was analyzed by GC-MS. The test conditions were: GCMS-QP2010plus (Shimadzu); Chromatographic column: DB-5MS (60m×0.25um×0.32mm) (Agilent); Heating rate: 60℃~10℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃; interface temperature: 300℃; Temper...

Embodiment 2

[0029] This example provides the determination of the contact activity of the essential oil of Seraphim bolotta against cotton aphids and psyllids.

[0030] (1) Determination of the contact activity of the essential oil of Artemisia bolota against cotton aphids

[0031] Measured by the leaf disc method, apply polytetrafluoroethylene to the four walls of a 12-well plate, after drying, spread the agar on the bottom of the plate, take fresh cotton leaves that have not been applied with pesticides, and punch the leaves to make a diameter of 3cm The circular leaf discs were soaked in the medicine for 10 seconds, dried and put into the leaves, and then 10 gossypis aphids were inserted into them, and placed in an incubator with light / darkness=16h / 8h, 25°C, and 60%-70% humidity. No essential oil solvent and pyrethrin were used as negative and positive controls respectively. Each treatment was repeated 6 times, and 10 cotton aphids were repeated for each repetition. After 24 hours aft...

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Abstract

The invention discloses insecticidal Seriphidium borotalense essential oil which is mainly prepared by the following steps: naturally drying the overground part of a Seriphidium borotalense plant in the shade, extracting for 6 hours by adopting a steam distillation method, extracting by adopting n-hexane to obtain an n-hexane layer, and adding anhydrous sodium sulfate to remove moisture. The invention also provides an application of the Seriphidium borotalense essential oil in insect killing. The Seriphidium borotalense essential oil can effectively realize contact killing of cotton aphids and phylloxera.

Description

technical field [0001] The invention belongs to the technical field of plant-derived pesticides, and relates to a plant of the genus Seraphim, in particular to an insecticidal Bolota Seraphim essential oil and an application thereof. Background technique [0002] With the continuous deepening of pesticide research, botanical pesticides have made great progress in recent years. They are safe for humans and animals, do not pollute the environment, are not easy to cause drug resistance, and are easy to degrade in the natural environment. Botanical pesticides mainly include active ingredients extracted from plants, plants themselves, and compounds and derivatives synthesized according to active structures. For example, azadirachtin, which has been industrially produced, is a secondary metabolite obtained from the neem plant neem; currently widely used natural insecticides, pyrethroid insecticides, are pyrethroids from the asteraceae plant pyrethrum. Modifications developed. Th...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P7/04
CPCA01N65/12A01N65/00Y02A50/30
Inventor 刘畅魏淑花王芳张蓉何嘉黄文广王颖朱蒙猛宋双
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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