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Application, extraction and determination method of raw fructus alpiniae oxyphyllae and salted fructus alpiniae oxyphyllae extract

A technology of extraction method and determination method, which is applied in the direction of medical preparations containing active ingredients, measuring devices, drug combinations, etc., can solve problems such as the unknown mechanism of signaling pathways, and achieve improvement of renal function damage, increase of content, and sensitivity high effect

Inactive Publication Date: 2021-11-26
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the application of Yizhiren extract in the treatment and prevention of diabetic complications DN, and its mechanism of action on each signaling pathway in DN is not yet known.

Method used

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  • Application, extraction and determination method of raw fructus alpiniae oxyphyllae and salted fructus alpiniae oxyphyllae extract
  • Application, extraction and determination method of raw fructus alpiniae oxyphyllae and salted fructus alpiniae oxyphyllae extract
  • Application, extraction and determination method of raw fructus alpiniae oxyphyllae and salted fructus alpiniae oxyphyllae extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The extraction method of embodiment 1-sheng Yizhiren and salt Yizhiren extracts comprises the following steps:

[0035] (1) Take Yanyizhiren and Shengyizhiren and pulverize them, and pass through a 40-mesh sieve to obtain Yanyizhiren and Shengyizhi powder;

[0036] (2) Add 7 times of the mass concentration of salt-yizhi kernel powder and raw-yizhi powder into 95% ethanol solution respectively, heat and reflux for extraction twice, each time for 1.5h, filter, combine the filtrates, recycle the ethanol by rotary evaporation, place Dry at 68°C to obtain Yanyizhiren extract and Shengyizhiren extract respectively.

Embodiment 2

[0037] The extraction method of embodiment 2-sheng Yizhiren and salt Yizhiren extracts comprises the following steps:

[0038] (1) Take Yanyizhiren and Shengyizhiren, pulverize them, and pass through a 60-mesh sieve to obtain Yanyizhiren and Shengyizhi powder;

[0039] (2) Add 9 times the mass concentration of salt-yizhi kernel powder and raw-yizhi powder into 95% ethanol solution respectively, heat and reflux for extraction twice, each time for 2.5h, filter, combine the filtrates, recycle the ethanol by rotary evaporation, and store Dry at 72°C to obtain Yanyizhiren extract and Shengyizhiren extract respectively.

[0040] The assay method of embodiment 2-sheng Yizhi kernel and salt Yizhi kernel extract

[0041]The UPLC-MS / MS method with high performance liquid chromatography-mass spectrometry is used to effectively determine the content of 9 chemical components in the extraction of Shengyizhiren and Yanyizhiren. method for the simultaneous determination of multiple componen...

Embodiment 3

[0049] Example 3 - Pharmacodynamic evaluation of Yizhiren before and after salt-burning on rats with diabetic nephropathy

[0050] 1. Extraction of Yizhiren

[0051] Dried the nuts and crushed them, and picked out the nuts and shells of the nuts. Take 500 g of raw Yizhiren powder and Yanyizhiren powder and place them in a 10L round-bottomed flask, add 8 times the amount of 95% ethanol, heat and reflux for extraction twice, each time for 2 hours, filter, combine the filtrates, and recover ethanol by rotary evaporation. Dry it in an oven at 70°C to make an extract for later use. 43.12g of the crude extract of Shengyizhiren and 44.36g of the crude extract of Yanyizhiren were respectively obtained.

[0052] 2. Modeling and grouping of DN rats

[0053] Male SD rats, body weight 200g-230g, SPF grade, all mice were housed at room temperature 25°C and relative humidity 50%-60% 12h light / dark cycle adaptive feeding for a week, no adverse reactions entered the experiment, the rats pa...

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Abstract

The invention relates to the technical field of plant extraction, in particular to application, extraction and determination method of raw fructus alpiniae oxyphyllae and salted fructus alpiniae oxyphyllae extract. According to the application of the raw fructus alpiniae oxyphyllae and salted fructus alpiniae oxyphyllae extract in preparation of drugs for treating diabetic nephropathy, by regulating expression of transforming growth factor-[beta]1 and [alpha]-smooth muscle actin, renal fibrosis and renal tubule injury can be treated, and coliform is regulated and controlled by promoting the generation of short-chain fatty acid SCFAs. The application provides an important basis for early prevention and effective treatment of diabetic nephropathy, reduction of vascular injury, effective treatment of diabetic complications and expansion of clinical application of the fructus alpiniae oxyphyllae extract.

Description

technical field [0001] The invention relates to the technical field of plant extraction technology, in particular to the application, extraction and determination methods of the raw and salted yizhiren extracts. Background technique [0002] Diabetic nephropathy (Diabetic nephropathy, DN) is one of the serious microvascular complications of diabetes. At present, the molecular pathogenesis of DN is not yet fully understood. As a typical metabolic disease, its occurrence and development include not only hemodynamic abnormalities and metabolic abnormalities, but also inflammation, oxidative stress, renin-angiotensin system ( RAS) activation and intestinal flora imbalance and many other pathological phenomena. [0003] The occurrence of diabetic nephropathy is often accompanied by hyperlipidemia, and the disorder of blood lipid metabolism is manifested by the increase of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-C) and high-density lipoprotein (HDL...

Claims

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Application Information

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IPC IPC(8): A61K36/9062A61P13/12A61P3/10A61P1/00A61P31/04G01N30/02G01N30/72G01N30/86A61K131/00
CPCA61K36/9062A61P13/12A61P3/10A61P1/00A61P31/04G01N30/02G01N30/72G01N30/8679A61K2236/333A61K2236/39A61K2236/51
Inventor 李永辉吴娇霞李立言吴毓皇徐晗
Owner HAINAN MEDICAL COLLEGE
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