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Preparation and extraction method of based on honey andcentella asiatica exosome

An extraction method and a technology of Centella asiatica, applied in the field of biomedicine, can solve problems such as difficulty in effectively breaking through the skin barrier, difficulty in skin absorption and utilization, etc., meet the requirements for use, treat stretch marks and obesity lines, and have good cold resistance and heat resistance. sexual effect

Inactive Publication Date: 2021-11-16
北京清大赛尔生物科技有限公司
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Problems solved by technology

[0006] However, these active substances in honey and centella asiatica extract are water-soluble ingredients, and the skin absorbs them through the gap of the stratum corneum. This gap is lipophilic (lipophilic), so it is difficult for hydrophilic ingredients to pass through the stratum corneum , it is still difficult to effectively break through the skin barrier in the application of skin care products, and it is difficult to be effectively absorbed and utilized by the skin

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  • Preparation and extraction method of based on honey andcentella asiatica exosome

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Embodiment 1

[0035] Such as figure 1 As shown, the present invention proposes a preparation and extraction method based on honey and Centella asiatica exosomes, including honey and Centella asiatica exosomes, and also includes active agents, pH regulators, humectants, stabilizers or preservatives one or more of

[0036] Preparation of extraction method, comprising the following steps:

[0037] S1: Add 200-250mL of sterilized PBS to honey and Centella asiatica, 200-250g to honey and Centella asiatica;

[0038] S2: After crushing with a crusher, centrifuge at 4°C and 3000×g for 30 minutes;

[0039] S3: filter to remove larger residues, and take the purple transparent solution in the middle layer;

[0040] S4: Centrifuge the purple transparent solution at 4°C and 10,000×g for 60 minutes to obtain the supernatant;

[0041] S5: Centrifuge the supernatant at 4°C and 120,000×g for 50 min;

[0042] S6: Use 1-2mL sterile PBS to resuspend the pellet, and centrifuge at 4°C and 150000×g for 60min...

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Abstract

The invention provides a preparation and extraction method based on the honey and the centella asiatica exosome, wherein the honey and the centella asiatica exosome are included, and one or more of an active agent, a pH regulator, a humectant, a stabilizer or a preservative are further included. The product is used for preventing, relieving and treating skin scars (striae gravidarum), has good cold resistance and heat resistance, can meet the use requirements under the condition of extreme temperature change, and can be used for treating striae gravidarum and obesity lines.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation and extraction method based on honey and centella asiatica exosomes. Background technique [0002] Animal and plant resources are one of the most important resources for human beings, and they are an important source of food, medicine and various living materials. In terms of drug application, different animals and plants produce different secondary metabolites, most of these secondary metabolites are biologically active, and are widely used in skin anti-aging (such as asiaticoside), anti-inflammatory soothing (such as bisabolol ), whitening and freckle removal (such as anthocyanins) and other skin care directions. However, the effects of animals and plants on the human body are far from limited to secondary metabolites. Plant exosomes also have a variety of similar human regulation effects, showing their important potential application value in skin care. [0003] With...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61P17/02C12N5/04A61K35/644
CPCA61K35/644A61K36/23A61P17/02C12N5/04A61K2300/00
Inventor 潘元明李俊
Owner 北京清大赛尔生物科技有限公司
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