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Salt-tolerant strain BY2G20 and application thereof

A technology of BY2G20 and strains, applied in the field of microorganisms, to achieve significant antagonism, improve plant height, and broaden the application field

Active Publication Date: 2021-11-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of a single strain, it is necessary to find new strains to enrich the strain resources of plant rhizosphere growth-promoting bacteria

Method used

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  • Salt-tolerant strain BY2G20 and application thereof
  • Salt-tolerant strain BY2G20 and application thereof
  • Salt-tolerant strain BY2G20 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1 strain identification

[0090] A strain that can promote the growth of maize was screened, which can grow well in 15% NaCl solution, and was identified by 16S rDNA and physiological and biochemical indicators. like figure 1 and 2 As shown, the colony and thalline characteristics of the strain BY2G20 are as follows: after being cultivated on LB medium, the colony of the colony BY2G20 is round, with moist protrusions on the surface, regular edges, milky yellow, and opaque.

[0091] The physiological and biochemical characteristics of the strain BY2G20 are: the strain BY2G20 has no oxidase activity and is negative; it cannot reduce nitrate to nitrite and is negative; methyl red is positive; no acetylmethylmethanol is produced, and V-P is negative; Indole production negative; mannitol available, positive; xylose unavailable, negative; cellobiose available, positive; arabinose available, positive; sorbitol available, positive; maltose available, Positive; lactos...

Embodiment 2

[0095] Embodiment 2 Bacterial strain BY2G20 is to the growth-promoting experiment of nutrient solution culture of corn

[0096] The preparation method of the bacterial solution: transfer the bacteria preserved on the slant to a test tube containing 5mL LB liquid medium, and culture it in a constant temperature shaker for 12 hours, the temperature: 37°C, the speed: 180rpm; the activated bacterial solution Inoculate 1% of the inoculum into 50mL LB liquid medium, put it into a shaker for 16 hours, temperature: 37°C, rotation speed: 180rpm, and make a bacterial solution.

[0097] Among them, LB liquid medium: yeast extract, 0.5%, peptone, 1%, sodium chloride, 1%, distilled water, 1000mL. Sterilize at 121°C for 20 minutes, pH7.0.

[0098] Use 75% alcohol to soak the Zhengdan 958 corn seeds for 5 minutes, fully shake the container, so that the alcohol removes the outer coating of the corn seeds. After pouring off the 75% alcohol, soak the corn seeds with 1% sodium hypochlorite for...

Embodiment 3

[0100] Example 3 Bacterial strain BY2G20 produces IAA and antagonizes Ralstonia solanacearum test

[0101] Detect the ability of strain BY2G20 to produce IAA and antagonize R. solanacearum, the results are as follows: Figure 4 and Figure 5 shown. The test results showed that the screened strain BY2G20 had the effect of producing IAA and antagonizing R. solanacearum.

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PUM

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Abstract

The invention provides a salt-tolerant bacillus BY2G20 and application thereof, and belongs to the technical field of microorganisms. The salt-tolerant bacillus obtained through screening can obviously improve the plant height, the root length, the stem diameter and the like in the corn growth period, effectively relieves or relieves the salt stress effect of the growth environment on corn plants, and effectively enriches strain resources of plant growth-promoting bacteria; and meanwhile, the strain also has IAA production performance and has a remarkable antagonistic effect on phytopathogens (such as ralstonia solanacearum), so that the application field of the strain is greatly widened, and the strain has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a salt-tolerant bacterial strain BY2G20 and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] As an important food crop, corn is widely planted in my country. Compared with traditional grain crops such as rice and wheat, corn has the characteristics of drought tolerance, cold resistance, strong environmental adaptability and high nutritional value. As a high-yield food crop in China, corn is widely used. In addition to being eaten directly, it can also be used in various food, medicine, textile, chemical and feed industries. The consumer mark...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/10A01N63/22A01P21/00A01P1/00C09K17/14C05F11/08C05G3/00C05G3/60C05G3/80C12R1/07C09K101/00
CPCC12N1/20C12P17/10A01N63/22C09K17/14C05F11/08C05G3/00C05G3/60C05G3/80C09K2101/00
Inventor 汪城墙杜秉海丁延芹王欣刘凯姚良同
Owner SHANDONG AGRICULTURAL UNIVERSITY
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