Preparation method of S-nicotine

Abstract

The invention relates to the technical field of biosynthesis, in particular to a preparation method of S-nicotine. According to the invention, amine oxidase is creatively utilized to oxidize 1-methylpyrrolidine into corresponding imine, and then the imine and nicotinic acid are condensed and decarboxylated under the catalysis of nicotine synthetase to obtain the final product S-nicotine. According to the method, the S-nicotine with specific chirality can be obtained through two-step reaction in a reaction system, the synthetic route is short, the yield is high, the reaction conditions are mild, and large-scale production is easy to realize; and meanwhile, the raw materials are wide in source, low in price, low in production cost and environmentally friendly, the production cost of nicotine is remarkably reduced, and the nicotine better meets the requirements of current green industrial production at the same time.

Description

technical field [0001] The invention relates to the technical field of biosynthesis, in particular to a preparation method of S-nicotine. Background technique [0002] Nicotine is an important component in tobacco, and it is also the core raw material for e-cigarette formulations and the synthesis of certain nicotine drugs. [0003] The traditional tobacco leaf cultivation, extraction, and purification methods occupy a large area and take a long time, and because the extraction inevitably contains other highly toxic costs, it will cause great harm to the human body. Therefore, the direct synthesis of chemical or biological processes has become an important way for the preparation of S-nicotine. [0004] Several common S-nicotine preparation methods: [0005] [0006] Route I: Preparation of racemic nicotine by chemical method. Using pyridine acetaldehyde as a raw material, a nicotine racemate is prepared through three-step chemical reactions, and then chiral resolution...

AI Technical Summary

Problems solved by technology

Although the route is relatively short and the yield is high, the production cost is high due to the use of expensive mysmin as the starting material

[0009] The above three classic S-nicotine preparation techniques can be concluded that although the two chemical preparation methods of route I and route II also use relatively cheap raw materials, the overall route is also Not long (three- or four-step reaction), but the chemical catalysts involved in the reaction are expensive (BF3, LDA, NaBH4), dangerous to operate (LDA, NaCN ) and other factors lead to high environmental and safety costs in its large-scale production

Route III uses mesmin as a raw material, utilizes imine reductase chiral reduction to obtain S-nornicotine (nornicotine), and then uses chemical methods to carry out methylation; the defect of this route is that it needs to use expensive mesmin as Raw materials, thus greatly increasing the overall production cost

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