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Compound microbial agent containing saline-alkaline-tolerant bacillus paralicheniformis and preparation method of compound microbial agent

A compound microbial agent, Bacillus licheniformis technology, applied in the field of microorganisms, can solve problems such as unsatisfactory disease control effects, unfavorable microbial colonization and growth, etc., to achieve strong salt-alkali tolerance and growth-promoting ability, strong antagonistic effect, and good application effect of effect

Pending Publication Date: 2021-11-09
东营市华科农业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) Since the saline-alkali environment is not conducive to the colonization and growth of microorganisms, when ordinary microbial fertilizers are applied in saline-alkali soil, the promotion effect on the growth of tomato and other crops and the control effect on crop diseases such as rice sheath blight are not ideal
[0006] (2) At present, when the products containing Bacillus paralicheniformis are used in practice, their growth promotion effect on tomato and other crops and the control effect on rice sheath blight and other crop diseases are not ideal, so they are rarely sold in the market

Method used

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  • Compound microbial agent containing saline-alkaline-tolerant bacillus paralicheniformis and preparation method of compound microbial agent
  • Compound microbial agent containing saline-alkaline-tolerant bacillus paralicheniformis and preparation method of compound microbial agent
  • Compound microbial agent containing saline-alkaline-tolerant bacillus paralicheniformis and preparation method of compound microbial agent

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preparation example Construction

[0043] Such as figure 1 As shown, the preparation method of the compound microbial bacterial agent containing saline-alkali-tolerant Bacillus paralicheniformis provided by the embodiments of the present invention comprises the following steps:

[0044] S101, activation and expansion of Bacillus paralicheniformis MIB-17 strain;

[0045] S102, solid-state fermentation of Bacillus paralicheniformis MIB-17 strain;

[0046] S103, preparation of bacteria powder of Bacillus megaterium ACCC 04314;

[0047] S104, preparation of Bacillus subtilis ACCC 19743 bacterial powder;

[0048] S105, preparation of compound microbial agent.

Embodiment 1

[0051] The invention discloses a composite microbial bacterial agent and a preparation method thereof. The composite microbial bacterial agent contains three functional bacteria: Bacillus paralicheniformis MIB-17, Bacillus megaterium ACCC 04314 and Bacillus subtilis ACCC 19743. The bacillus paralicheniformis MIB-17 is preserved in the General Microorganism Center of China Microbial Strain Preservation Management Committee, and the preservation number is CGMCC No.22607. It has excellent characteristics such as salt-alkali tolerance, promotion of crop growth, improvement of soil enzyme activity, and antagonism of crop pathogenic bacteria. or beneficial effects, and can be used to produce microbial fertilizers, especially microbial fertilizers suitable for saline-alkali soils. The Bacillus megaterium ACCC 04314 and Bacillus subtilis ACCC 19743 were purchased from China Agricultural Microorganism Culture Collection and Management Center.

[0052] The partial sequence of the 16S r...

Embodiment 2

[0056] Example 2: Screening of Bacillus paralicheniformis MIB-17

[0057] (1) Isolation of saline-alkali-tolerant strains

[0058] Weigh 10g of saline-alkali soil, put it into an Erlenmeyer flask containing 90mL of sterile water and 10-20 glass beads, and shake at 180r / min for 30min. Diluted sequentially to 10 by serial dilution -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 . Pipette 0.1 mL each of the dilutions of different concentrations and spread evenly on the LB plate, and incubate at 37°C for 48-72h. Pick different colonies and streak them on the LB plate, culture at 37°C for 48-72h to obtain a single colony. The formula of the LB medium is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, agar 20g / L, pH 7.5.

[0059] Inoculate the isolated single colonies on LB saline-alkali medium plate in turn, culture at 37°C for 48-72 hours, observe and record the growth status of the strains, and select the strains with relatively better growth for subsequent experi...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a compound microbial agent containing saline-alkaline-tolerant bacillus paralicheniformis and a preparation method thereof. The compound microbial agent containing the saline-alkaline-tolerant bacillus paralicheniformis is prepared from, by mass, 50 parts of bacillus paralicheniformis MIB-17 strain culture, 15 parts of bacillus megatherium ACCC 04314 strain culture, 15 parts of bacillus megatherium ACCC 19743 strain culture, 15 parts of bacillus subtilis ACCC 19743 strain culture, 10 parts of humic acid and 10 parts of compound trace elements. According to the invention, a bacillus parlicheniformis MIB-17 strain with relatively strong saline-alkaline tolerance and crop growth promoting capability is used as a main functional strain, preferably, a bacillus megatherium ACCC 04314 strain and a bacillus subtilis ACCC 19743 strain are matched, and the compound microbial agent is prepared by combining the strains with humic acid and trace elements. The application effect is better than that of a single fungicide.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound microbial agent containing saline-alkali-tolerant Bacillus paralicheniformis and a preparation method thereof. Background technique [0002] At present, soil salinization is one of the most serious environmental problems in the world. More than 20% of the cultivated land and 33% of the irrigated land in the world have salinization problems, and the rate of increase is 2 million hectares per year. Saline-alkali land is widely distributed in China. Different types of saline-alkali land are distributed in the inland areas of Northeast, Northwest and North China and coastal plains, with a total area of ​​about 3.6×10 7 hm 2 , occupying 4.9% of the available land area of ​​the country. Saline-alkali land is an important reserve resource of cultivated land. To expand agricultural production, it is very important to rationally develop and utilize saline-a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/60C05G3/80C12R1/11C12R1/125C12R1/07
CPCC12N1/20C05G3/60C05G3/80C05D5/00C05D9/02C05F11/02Y02W30/40
Inventor 刘丽英邵光杰古今朝孙中涛侯汉学崔树君徐燕徐风英
Owner 东营市华科农业科技有限公司
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