Bacillus subtilis water dispersible granules and preparation method and application thereof

A technology of Bacillus subtilis and dispersible granules, applied in the field of agricultural microorganisms, can solve the problems of low suspension rate of water dispersible granules, unfavorable use of leaf spray, high local concentration, etc., and achieve high wet sieving pass rate, easy to use, and high granulation rate high effect

Pending Publication Date: 2021-11-05
山东京青农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent document CN102870822B Example 2 No organic wetting agent was added in the process of preparing Trichoderma chlamydospore biopesticide water-dispersible granules, and the prepared water-dispersible granules had a low suspension rate (43.2%), which was unfavorable for leaf spraying. And the local concentration will be too high, which will cause burns to the leaves

Method used

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  • Bacillus subtilis water dispersible granules and preparation method and application thereof
  • Bacillus subtilis water dispersible granules and preparation method and application thereof
  • Bacillus subtilis water dispersible granules and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The method of one kind of culture of Bacillus subtilis, the following steps:

[0035] (1) activating species: The cryopreservation Bacillus subtilis strains were each inoculated on a nutrient agar plate medium and incubated 30h at 37 [deg.] C;

[0036] (2) Preparation seed liquid: picked in step (1) is transferred to large colonies on the seed medium, shaking 260 rpm, 16h culture at 37 ℃, as a seed liquid;

[0037] The seed medium composition and weight percentage: glucose 1.5%, peptone 1%, yeast extract 0.7%, beef extract 0.7%, NaCl 0.5%, the balance being water;

[0038](3) Fermentation culture: The seed liquid prepared in step (2) in the fermentation medium in accordance with 3% of the inoculation amount, the amount of ventilation is 1 Vm, the stirring speed is 300 rpm, the temperature is 37 ° C, the culture time is 33 h, resulting in the fermentation broth ;

[0039] The composition ingredients of the fermentation medium and its weight percent: 7% corn starch (65 ° C hy...

Embodiment 2

[0042] Bacillus culture method, steps below:

[0043] (1) Activation of strains: The Bacillus strain preserved by low temperature was inoculated on nutrient agar plate culture medium, and 33 h was cultured at 36 ° C;

[0044] (2) Seed liquid prepared: Picking steps (1) The large colonies are transferred to seed medium, and the shaker is 260 rpm, and at 36 ° C for 18 hours, as seed liquid;

[0045] The composition ingredients of the seed medium and its weight are: glucose 1.6%, protein 胨 1.2%, yeast powder 0.7%, beef paste 0.5%, and 0.5% sodium chloride;

[0046] (3) Fermentation culture: The seed liquid prepared in step (2) in the fermentation medium in accordance with 5% of the inoculation amount, the amount of ventilation is 1.2 Vvm, the stirring speed is 260 rpm, the temperature is 36 ° C, the culture time is 36 h, resulting in fermentation liquid;

[0047] The composition ingredients of the fermentation medium and its weight percent: 9% corn starch (hydrolyzate 1H), 7000 vital...

Embodiment 3

[0050] Bacillus culture method, steps below:

[0051] (1) Activation of strains: Paciline strains preserved in the Nutrient agar plate medium were cultured at 36 ° C for 30 h.

[0052] (2) Seed liquid prepared: Picking steps (1) The large colonies are transferred to seed medium, and the shaker is 260 rpm, and at 36 ° C for 20 h, as a seed liquid;

[0053] The composition ingredients of the seed medium and its weight are: glucose 1.7%, protein 胨 1.3%, yeast powder 0.8%, beef paste 0.5%, and 0.5% sodium chloride;

[0054] (3) Fermentation culture: According to 3% inoculation amount, the seed liquid prepared in the fermentation medium is 1 Vvm, the amount of ventilation is 1 Vm, the stirring speed is 260 rpm, the temperature is 36 ° C, the culture time is 30 h, resulting in the fermentation broth ;

[0055] The composition ingredients of the fermentation medium and its weight percent were: 5% corn starch (70 ° C hydrolyzed 1H), 7000 vital unit amylase (0.02% of starch dosage), 5% cor...

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Abstract

The invention provides agricultural bacillus subtilis water dispersible granules and a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus subtilis water dispersible granules are fermented by adopting a specific culture medium, and the viable count is high. Spray drying is carried out in a manner of adding a protective agent, and the drying yield is high. Finally, granulation is carried out with a carrier to form a water dispersing agent. According to the invention, the prepared bacillus subtilis water dispersible granules are not added with an exogenous organic wetting agent and a dispersing agent, normal germination of spores is not affected, and plant growth is not harmed. According to the bacillus subtilis water dispersible granules prepared by the invention, the utilization rate of a fertilizer can be effectively improved, and the use amount of the fertilizer is reduced.

Description

Technical field [0001] The present invention relates to a Bacillus subtilis agricultural water dispersible granule, as well as its preparation method and application, microorganisms belonging to the technical field of agriculture. Background technique [0002] Chemical fertilizers played an irreplaceable role in promoting the development of food and agricultural production, convoy escort for the income of farmers and harvest food. Because fertilization skill levels, unreasonable fertilization is widespread, blind excessive use of chemical fertilizers, not only a waste of non-renewable resources and pollution of the environment, and the quality and yield of fruits and vegetables have not been a corresponding increase. [0003] Microorganism belonging to renewable resources, and more beneficial species, environmental pollution, in recent years, more and more varieties of microbial fertilizer, manure is the major structural development of the backbone. Bacillus subtilis is widely di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A01N63/22A01N25/12A01P21/00C05G3/00C05G3/60C05G3/80C05G5/12C12R1/125
CPCC12N1/20C12N1/04A01N63/22A01N25/12C05G3/00C05G3/60C05G3/80C05G5/12C05B7/00C05C11/00C05D1/02C05F11/00C05F11/02
Inventor 郭中信郭仲成王茂超郭倩
Owner 山东京青农业科技有限公司
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