Traditional Chinese medicine fermented feed for improving intestinal immunity of aquaculture animals and preparation method of traditional Chinese medicine fermented feed
A fermented feed and aquaculture technology, applied in animal feed, animal feed, pharmaceutical formula, etc., can solve problems such as intestinal diseases, improve intestinal immunity, improve economic benefits, and have no toxic and side effects
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Embodiment 1
[0031] Preparation of compound microbial agent
[0032] The strains in the examples of the present invention were all purchased from the China General Microorganisms Collection and Management Center; among them, Lactobacillus plantarum CGMCC1.573; Bacillus subtilis CGMCC1.8715; Bacillus licheniformis CGMCC1.518.
[0033] Preparation:
[0034] (1) Culture medium preparation
[0035] Take LB liquid culture medium (peptone 10.0g, yeast extract 3.0g, NaCl 5.0g, distilled water 1.0L; pH7.0), add 20% glucose aqueous solution, high temperature sterilization for later use;
[0036] (2) Preparation of bacteria agent
[0037] Activate Lactobacillus plantarum in a conventional way and then increase the content ≥ 10 8 CFU / g of bacterial liquid was inoculated into the above-mentioned sterilized medium, and the amount of bacterial liquid added was 10% of the volume of the medium; fermented at 32°C for 30 hours;
[0038] Activate Bacillus subtilis in a conventional way and then make the ...
Embodiment 2
[0042] A traditional Chinese medicine fermented feed for improving intestinal immunity of aquaculture animals, the fermented feed raw material is prepared from raw materials of the following quality:
[0043] Fish meal 25kg, shrimp meal 5kg, peanut meal 15kg, soybean meal 15kg, squid meal 3kg, wheat bran 20kg, soybean lecithin 2kg, fish oil 4kg, brown sugar 20kg, ammonium sulfate 2kg, potassium dihydrogen phosphate 2kg, Haematococcus pluvialis powder 2kg, Chinese medicine powder 8kg;
[0044] The traditional Chinese medicine powder contains the following ingredients in parts by mass:
[0045] 3 parts capillary, 10 parts turmeric, 8 parts fireweed, 8 parts dandelion, 8 parts Andrographis paniculata, 8 parts ginkgo biloba, 3 parts milk thistle, 15 parts licorice.
[0046] Preparation:
[0047] Take the raw materials according to the amount, crush the traditional Chinese medicine to 80 mesh, and mix all the raw materials evenly after crushing;
[0048] Add water with 85% of th...
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