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Common poison screening identification and confirmation standard, screening method and kit

A technology for confirming standards and kits, applied in the field of poison screening, to achieve the effects of ensuring sensitivity, improving screening accuracy, high screening sensitivity and accuracy

Pending Publication Date: 2021-11-02
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Common poison screening identification and confirmation standard, screening method and kit
  • Common poison screening identification and confirmation standard, screening method and kit

Examples

Experimental program
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Effect test

Embodiment 1

[0065] Establish a database for toxicant screening: select 354 common organic toxicants, including 22 kinds of drugs, 33 kinds of medicines, 248 kinds of pesticides, 12 kinds of rat poisons, 4 kinds of veterinary drugs, 13 kinds of toxic alkaloids, and 22 kinds of mycotoxins. - Prepare standard sample working solutions containing each poison to be tested in water, inject them into the chromatographic mass spectrometer for analysis, analyze in full scan mode, use the QualBrowser software integrated in Xcalibur 4.0 to obtain the precursor ions and characteristic fragment ions of the analyte Accurate m / z, retention time, ion abundance ratio and isotope matching degree, combined with poison name, molecular formula, CAS number (Chemical Abstracts Service) and other information are imported into TraceFinder 5.1 to establish a poison screening database for poison identification and confirmation.

[0066] Based on Embodiment 1, the present application also provides a computer-readable ...

Embodiment 2

[0069] Establish poison screening identification and confirmation standards: precursor ion m / z is used for identification, threshold coverage (Threshold Override) > 5000, signal-to-noise ratio threshold (S / N Ratio Threshold) > 5, mass tolerance (Mass tolerance ) 2 . Isotopic Pattern is used for Confirmation, Fit Threshold > 70%, Allowed Mass Deviation < 5ppm, Allowed Intensity Deviation < 20%.

[0070] Based on Embodiment 2, the present application also provides a computer-readable storage medium.

[0071] A computer-readable storage medium. A computer program is stored in the computer-readable storage medium. When the computer program is executed by a processor, the method steps of Embodiment 2 are implemented.

Embodiment 3

[0073] Establish a database for toxicant screening: select 354 common organic toxicants, including 22 kinds of drugs, 33 kinds of drugs, 248 kinds of pesticides, 12 kinds of rat poisons, 4 kinds of veterinary drugs, 13 kinds of toxic alkaloids, and 22 kinds of mycotoxins. - Prepare standard sample working solutions containing each poison to be tested in water, inject them into the chromatographic mass spectrometer for analysis, analyze in full scan mode, use the QualBrowser software integrated in Xcalibur 4.0 to obtain the precursor ions and characteristic fragment ions of the analyte Accurate m / z, retention time, ion abundance ratio and isotope distribution, combined with poison name, molecular formula, CAS number (Chemical Abstracts Service) and other information are imported into TraceFinder 5.1 to establish a poison screening database for poison identification and confirmation.

[0074]Establish poison screening identification and confirmation standards: precursor ion m / z i...

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Abstract

The invention discloses a common poison screening identification and confirmation standard, a screening method and a kit, the common poison screening identification and confirmation standard comprises a precursor ion m / z used for identification, a threshold coverage greater than 5000, a signal-to-noise ratio threshold greater than 5, and a mass deviation less than 5 ppm; the retention time is used for confirmation, and the window coverage is + / -1min; the fragment ions are used for confirmation, the minimum number of the fragment ions is 1, the peak intensity threshold value is 1000, and the MS Order is set as MS2; the isotope mode is used for confirmation, the matching threshold value is larger than 70%, and the allowable mass deviation and the allowable intensity deviation are smaller than 20%. When a poisoning event occurs and a judicial poison test occurs, the database provided by the invention can realize rapid, accurate and broad-spectrum screening of organic poison at a poisoning level.

Description

technical field [0001] This application belongs to the field of poison screening, and relates to the screening of poison exposure and poisoning biomarkers, in particular, it relates to a kit-based ultra high performance liquid chromatography-quadrupole for 354 common organic poisons in blood, urine and hair Rod Orbitrap Mass Spectrometry Rapid Screening Method. Background technique [0002] In recent years, poisoning incidents have occurred frequently and the fatality rate is high. In my country, poisoning is the leading cause of death in public health incidents, and the number of poisoning deaths accounts for more than 10% of the total deaths. Among them, the proportion of poisoning incidents caused by organic poisons is increasing, and the number of poisonings is also rising. The organic poisons that cause poisoning are mainly drugs, pesticides, alcohol, veterinary drugs, rat poisons, toxic alkaloids, mycotoxins, etc. About 60% of poisoning deaths are related to organic p...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
Inventor 邹晓莉任建伟赵璇罗新月骆春迎徐布一王欢博李卓雅黄馨仪郑波张启福
Owner SICHUAN UNIV
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