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Product based on nontuberculous-mycobacterium purified protein derivative and recombinant mycobacterium tuberculosis fusion protein, application and use method

A technology of protein derivatives and mycobacteria, applied in the field of biological proteins, achieves the effects of short identification time, guaranteed sensitivity and high accuracy

Active Publication Date: 2021-10-29
成都可恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problem that it takes up to 4-6 weeks to distinguish through the growth form of bacteria in the traditional identification method

Method used

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  • Product based on nontuberculous-mycobacterium purified protein derivative and recombinant mycobacterium tuberculosis fusion protein, application and use method
  • Product based on nontuberculous-mycobacterium purified protein derivative and recombinant mycobacterium tuberculosis fusion protein, application and use method
  • Product based on nontuberculous-mycobacterium purified protein derivative and recombinant mycobacterium tuberculosis fusion protein, application and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] This example discusses the combination of pure protein derivatives of non-tuberculosis mycobacterium and recombinant tuberculosis fusion protein to induce delayed hypersensitivity in guinea pigs sensitized to the attenuated strain of mycobacterium tuberculosis (CMCC93020), so as to further improve the technical scheme illustrate.

[0046] S1: guinea pig

[0047] SPF grade guinea pigs with a body weight of 300-500g were raised in IVC cages (3 guinea pigs per cage, the litter was changed twice a week, free access to food and water).

[0048] S2: skin test

[0049] Tuberculin pure protein derivative skin test negative guinea pigs.

[0050] S3: Allergens

[0051] Take the sensitizer (50mg / mL) of the attenuated strain of Mycobacterium tuberculosis (CMCC93020), and sterilize it at 121°C for 20min.

[0052] S4: Sensitization

[0053] Inject 0.2mL of the allergenic bacteria solution (50mg / mL) in the groin, and perform the second sensitization 3 weeks after the first sensit...

Embodiment 2

[0060] This example discusses the combination of pure protein derivatives of non-tuberculosis mycobacterium and recombinant tuberculosis fusion protein against Mycobacterium kansasii (CMCC 95013) and Hai Guinea pigs sensitized with mycobacterium (CMCC95014) and mycobacterium simianum (CMCC95015) induced delayed hypersensitivity reactions to further illustrate this technical solution.

[0061] S1: guinea pig

[0062] SPF grade guinea pigs with a body weight of 300-500g were raised in IVC cages (3 guinea pigs per cage, the litter was changed twice a week, free access to food and water).

[0063] S2: skin test

[0064] Tuberculin pure protein derivative skin test negative guinea pigs.

[0065] S3: Allergens

[0066] Allergens (50 mg / mL) of Mycobacterium kansasii (CMCC 95013), Mycobacterium marinum (CMCC95014) and Mycobacterium simianus (CMCC95015) were taken and sterilized at 121°C for 20 minutes.

[0067] S4: Sensitization

[0068] Inject 0.2mL of the allergenic bacteria so...

Embodiment 3

[0079] This example discusses the combination of pure protein derivatives of non-tuberculosis mycobacterium and recombinant tuberculosis fusion protein against Mycobacterium gordonii (CMCC 95018), the main pathogenic bacteria of non-tuberculosis mycobacterium group II (slow growth, dark color production), Guinea pigs sensitized with Mycobacterium scrofula (CMCC 95019) and Mycobacterium scrofula (CMCC95017) induced delayed hypersensitivity reactions to further illustrate this technical solution.

[0080] S1: guinea pig

[0081] SPF grade guinea pigs with a body weight of 300-500g were raised in IVC cages (3 guinea pigs per cage, the litter was changed twice a week, free access to food and water).

[0082] S2: skin test

[0083]Tuberculin pure protein derivative skin test negative guinea pigs.

[0084] S3: Allergens

[0085] Allergens (50 mg / mL) of Mycobacterium kansasii (CMCC 95013), Mycobacterium marinum (CMCC 95014) and Mycobacterium simianus (CMCC95015) were taken and ste...

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Abstract

The invention discloses a product based on a nontuberculous-mycobacterium purified protein derivative and a recombinant mycobacterium tuberculosis fusion protein, application and a use method and belongs to the technical field of biological proteins. Tuberculosis and a NTM disease can be rapidly and accurately identified through combined use of the nontuberculous mycobacterium purified protein derivative and the recombinant mycobacterium tuberculosis fusion protein (EEC), and when delayed hypersensitivity reactions of the nontuberculous mycobacterium purified protein derivative and the recombinant mycobacterium tuberculosis fusion protein are all positive, the tuberculosis is determined; and when the delayed hypersensitivity reaction of the nontuberculous-mycobacterium purified protein derivative is positive and the delayed hypersensitivity reaction of the recombinant mycobacterium tuberculosis fusion protein is negative, the NTM disease is determined. The invention provides the product based on the nontuberculous-mycobacterium purified protein derivative and the recombinant mycobacterium tuberculosis fusion protein and the application of the product, so that the tuberculosis and the NTM disease are quickly and accurately identified, and a reliable basis is provided for the aspects of early diagnosis, treatment and the like of patients.

Description

technical field [0001] The invention relates to a protein product and its application, in particular to a product, application and use method based on a non-tuberculous mycobacterium pure protein derivative and a recombinant tuberculosis fusion protein, belonging to the technical field of biological proteins. Background technique [0002] Tuberculosis is a chronic infectious disease that seriously endangers human and animal health. In 2019, there were 1.4 million deaths from tuberculosis worldwide. According to the 2020 global tuberculosis annual report released by the WTO: the number of global tuberculosis cases in 2019 is estimated to be about 10 million (8.9-11 million); among them, India is 2.64 million, accounting for 26%; Indonesia is 846,000, accounting for 8.5%; China is 833,000, accounting for 8.5%. 8.4%. The number of tuberculosis patients in China ranks third in the world, and it is a country with a high burden of tuberculosis. [0003] For non-tuberculous mycob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00C12N1/20C12R1/32
CPCA61K49/0006C12N1/20
Inventor 孙超伟
Owner 成都可恩生物科技有限公司
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