Anhydrous yeast essence filtrate and preparation method thereof
A technology of filtrate and yeast, which is applied in the field of anhydrous yeast essence filtrate and its preparation, can solve the problems of unsatisfactory product effect, insufficient skin absorption rate, user troubles, etc., and achieve good wrinkles, improve skin dryness, and remove wrinkles.
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Embodiment 1
[0031] 1. Preparation of Bifida yeast fermentation lysate
[0032] (1) Inoculate Bifidobacterium longum in the medium for anaerobic fermentation culture, the inoculum size is 10% (V:V), the initial pH is 6.6-7.0, the culture temperature of anaerobic fermentation is 37-40°C, the time For 13h static culture. The mass ratio of the fermentation medium is (mass / volume): yeast powder 1.5%, glucose 2.0%, casein peptone 1.5%, beef peptone 1.0%, hydrolyzed milk protein 1.5%, anhydrous sodium acetate 0.5%, triammonium citrate 0.2 %, Tween-80 0.1%, magnesium sulfate 0.05%, manganese sulfate 0.02% and dipotassium hydrogen phosphate 0.05%.
[0033] (2) After the culture is over, shake the bacterial liquid slightly, and use the ultrasonic crushing method to break the cell wall under the condition of low temperature, ultrasonic 5-6s / time, and cycle 15 times.
[0034] (3) After crushing, add a small amount of suspending agent and gently pipette to make it completely fused, centrifuge at hig...
Embodiment 2
[0069] In order to study the appropriate concentration of Bifida yeast fermentation lysate, yeast extract, and yeast fermentation product filtrate extract, we used the control variable method for research. Randomly select 180 trial subjects aged 20-40, including 90 males and 90 females, and randomly divide them into three groups, namely the Bifida yeast fermentation lysate experimental group, yeast extract experimental group, and yeast fermentation product filtrate The extract experimental group, each group was randomly divided into 6 groups on average.
[0070] In the yeast extract experimental group, the concentration of yeast extract was set to 6 concentration gradients of 0, 1, 2, 3, 4, and 5 mg / ml, in which the yeast fermentation product filtrate extract and Bifida yeast lysate extract The addition amount of each was set to 0.2mg / ml.
[0071] In the experimental group of yeast fermentation product filtrate extract, the concentration of yeast fermentation product filtrate...
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