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Marine fish skin collagen oligopeptide and its preparation method and application

A technology of oligopeptide and fish skin, which is applied in the field of marine fish skin collagen oligopeptide preparation, can solve the problems of heavy fishy smell, achieve long shelf life, improve the degree of hydrolysis, and be convenient to carry

Active Publication Date: 2022-03-25
GUANGZHOU TIANQI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing methods for preparing marine fish oligopeptides have problems such as heavy fishy smell, which limits their application.

Method used

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  • Marine fish skin collagen oligopeptide and its preparation method and application

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Effect test

Embodiment 1

[0032] Embodiment 1, a kind of preparation method of marine fish skin collagen oligopeptide

[0033] The preparation method of the marine fish skin collagen oligopeptide comprises the following steps:

[0034] S1 Wash the deep-sea salmon skin with water, cut it into small pieces of 0.2cm×0.2cm, add n-hexane for degreasing treatment. 10h, filter, clean with water to obtain degreasing fish skin, add a mass concentration of 3% sodium chloride aqueous solution in the degreasing fish skin, the addition of sodium chloride aqueous solution is 10 times the quality of the degreasing fish skin, Soak at 4°C for 5 hours, stir at a speed of 100r / min during the soaking process, and filter to obtain the pretreated fish skin;

[0035] S2 adds deodorizing liquid to the fish skin after pretreatment obtained in step S1, the addition amount of deodorizing liquid is 4 times of the quality of fish skin after pretreatment, stirs evenly, places 1h, carries out steam treatment, steams on steam under ...

Embodiment 2

[0040] Embodiment 2, a kind of preparation method of marine fish skin collagen oligopeptide

[0041] The preparation method of the marine fish skin collagen oligopeptide comprises the following steps:

[0042] S1 Wash the deep-sea salmon skin with water, cut it into small pieces of 0.2cm×0.2cm, add n-hexane for degreasing treatment, the amount of n-hexane added is 8 times the weight of the fish skin, the degreasing temperature is 10°C, and the degreasing time is 15h, filter, clean with water, get the fish skin after degreasing, add the sodium chloride aqueous solution that mass concentration is 3% in the fish skin after degreasing, the add-on of sodium chloride aqueous solution is 13 times of the fish skin quality after degreasing, at Soak at 10°C for 8 hours, stir at a speed of 150r / min during the soaking process, and filter to obtain the pretreated fish skin;

[0043] S2 adds deodorizing liquid to the pretreated fish skin obtained in step S1, the addition of the deodorizing...

Embodiment 3

[0048] Embodiment 3, a kind of preparation method of marine fish skin collagen oligopeptide

[0049] The preparation method of the marine fish skin collagen oligopeptide comprises the following steps:

[0050] S1 Wash the deep-sea salmon skin with water, cut it into small pieces of 0.2cm×0.2cm, add n-hexane for degreasing treatment, the amount of n-hexane added is 7 times the weight of the fish skin, the degreasing temperature is 6°C, and the degreasing time is 12h, filter, clean with water to obtain degreased fish skin, add a mass concentration of 3% sodium chloride aqueous solution in the degreased fish skin, the addition of sodium chloride aqueous solution is 12 times the quality of the degreased fish skin. Soak at 6°C for 7 hours, stir at a speed of 120r / min during the soaking process, and filter to obtain the pretreated fish skin;

[0051] S2 adds deodorizing liquid to the pretreated fish skin obtained in step S1, the addition of the deodorizing liquid is 5 times the qua...

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Abstract

The invention belongs to the technical field of preparation of marine fish skin collagen oligopeptide, in particular to a marine fish skin collagen oligopeptide and a preparation method and application thereof. The preparation method of the marine fish skin collagen oligopeptide of the present invention comprises the steps of cutting fish skin into small pieces, carrying out degreasing treatment, adding sodium chloride aqueous solution, soaking, filtering, adding deodorant liquid to the pretreated fish skin, and steaming Treatment: freeze-dry the fish skin after steam treatment, pulverize, add water to the fish skin powder, homogenize, adjust the pH of the homogenized material, add compound enzymes, enzymolysis, carry out ultrafiltration treatment on the obtained enzymolysis solution, and collect the retentate , the obtained retentate is subjected to electrodialysis treatment, and the desalted peptide liquid is concentrated and dried to obtain it. The marine fish skin collagen oligopeptide prepared by the invention has good taste and no fishy smell, and is applied in the preparation of oral liquid, and the obtained product has good taste. And the oral liquid containing the marine fish skin collagen oligopeptide provided by the present invention has a significant anti-fatigue effect and can relieve physical fatigue.

Description

technical field [0001] The invention belongs to the technical field of marine fish skin collagen oligopeptide preparation, and in particular relates to a marine fish skin collagen oligopeptide, a preparation method and application thereof. Background technique [0002] my country is the largest country in the world in importing and exporting processed fish products, and the annual import and export volume of fish products is as high as 50% of the world's total. And in the process of fish industry processing, the leftovers of accompanying a large amount of fish accounts for about 30-50% of fish weight, wherein the proportion of fish skin is higher. Most of these discarded fish skins are used as agricultural fertilizer or feed, and most of them are discarded directly as garbage, which not only causes a waste of resources, but also causes serious environmental pollution. In recent years, the research and development of collagen, peptides and oligopeptides using fish skin as ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34A23L33/18
CPCC07K14/78C12P21/06A23L33/18A23V2002/00A23V2200/30A23V2250/038A23V2250/21A23V2250/5062A23V2250/55
Inventor 谢涛王中振谢骞刘家生
Owner GUANGZHOU TIANQI BIO TECH
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