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Sporopollen-nanogold compound as well as preparation method and application thereof

A sporopollenin and nano-gold technology, applied in the field of non-natural enzyme mimics, can solve problems such as easy aggregation, reduced catalytic performance, and insufficient stability of nano-gold materials

Active Publication Date: 2021-10-15
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single nano-gold material is often not stable enough and easy to aggregate, which reduces its catalytic performance. Therefore, composite materials such as graphene / Au and Bp / Au have been studied.
Although these composite materials improve the catalytic ability of gold nanoparticles, they need to be freshly prepared, and the samples cannot be stored for a long time, which is not conducive to industrialization, and cannot be recycled and is not friendly to the environment.

Method used

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  • Sporopollen-nanogold compound as well as preparation method and application thereof
  • Sporopollen-nanogold compound as well as preparation method and application thereof
  • Sporopollen-nanogold compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Such as figure 1 Shown, the concrete steps that present embodiment prepares sporopollenin are as follows:

[0029] (1) Weigh an appropriate amount of camellia pollen and add clean water, after dispersing in ultrasonic, add a large amount of clear water to filter and wash the camellia pollen three times, then add deionized water to wash the camellia pollen once, and finally drain the pollen in the Dry at 45°C to obtain surface-removed pollen.

[0030] (2) Take part of the pollen on the above-mentioned surface, add an appropriate amount of acetone, heat and stir at 70°C and 250rpm for 4 hours, then stop heating and stirring, and filter with suction; then add fresh acetone, and heat at 70°C and 250rpm After heating and stirring for 2 hours, remove the acetone by suction filtration (6 hours of shared acetone treatment); then add hot absolute ethanol for suction filtration and washing three times to remove residual acetone, add hot deionized water for suction filtration and...

Embodiment 2

[0034] After mixing 50 mL of water and 250 μL of chloroauric acid solution (50 mM) and heating to boiling, slowly add 3.5 mL of 1% sodium citrate solution dropwise to the solution, and continue heating for 20 minutes to obtain the following: figure 2 The gold nanoparticles shown were stored at 4°C after cooling naturally for later use. Measure its ultraviolet absorption spectrum, by figure 2 (a) It can be seen that the nano-gold is purple-red, and the ultraviolet absorption peak is 520nm.

[0035] Weigh a certain amount of sporopollenin prepared in Example 1, add ultrapure water to prepare it into a 1 mg / mL sporopollenin dispersion, and disperse with an ultrasonic cleaner. The sporopollenin dispersion and the concentration of the chloroauric acid solution of 0.1, 0.5, 1.0, 1.5, 2.0mmol / L were ultrasonicated for 2h under 90% power to make Sp-Au (i.e. the sporopollenin-nanogold of the present invention complex), the volume ratio of sporopollenin dispersion to chloroauric aci...

Embodiment 3

[0038] Add HAc-NaAc buffer (0.2M, pH=4.0), TMB, catalyst (i.e. Sp-Au, figure 2 C3) and G in 2 o 2 , put into the ultraviolet spectrophotometer to scan, and measure the change of the absorbance of the mixture at 652nm with time. Immobilized Sp-Au (1mg / mL, 400μL), H 2 o 2 (final concentration 10mM), change the TMB concentration (50-600μM), the changed solution volume is supplemented with buffer solution, the total volume of the mixed solution is 3mL, the change of the absorbance of the detection system at 652nm is obtained, and the kinetic curve with TMB as the substrate is obtained . Fix Sp-Au (1mg / mL, 400μL), TMB (final concentration 600μM), change H 2 o 2 Concentration (0.5-50mM), the changed solution volume is supplemented with buffer solution, the total volume of the mixed solution is 3mL, and the change of the absorbance of the detection system at 652nm is obtained in the form of H 2 o 2 Kinetic curve for the substrate. Fit the kinetic curve, according to the Mie...

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Abstract

The invention discloses a sporopollen-nanogold compound and a preparation method and application thereof. The sporopollen-nanogold compound is prepared from sporopollen and chloroauric acid, the chloroauric acid reacts with reducing groups including hydroxyl on the surface of the sporopollen to generate nanogold on the surface of the sporopollen in situ, and hydrogen peroxide can be catalyzed to subject 3,3',5,5'-tetramethylbenzidine to color change reaction. The compound can be used for glucose detection.

Description

technical field [0001] The invention belongs to the technical field of non-natural enzyme mimics, and in particular relates to a sporopollenin-nano-gold complex and a preparation method and application thereof. Background technique [0002] As a kind of catalyst, enzyme has the properties of high catalytic efficiency and specific selection under mild and suitable conditions, and has great practical application value in the fields of medicine, chemical industry, agriculture, biosensor development, environmental protection and food industry. However, most natural enzymes are proteins, lack long-term stability, are easily denatured and inactivated by factors such as temperature and pH, and are easily decomposed by proteases in the environment. In addition, the process of preparing, purifying, and storing enzymes is cumbersome, costly, and time-consuming, while enzyme mimics have high catalytic properties, good stability, low preparation costs, and simple preparation steps, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/78G01N21/33
Inventor 孙莉萍温雪琴徐宛
Owner XIAMEN UNIV
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