Microscopic circular polarization fluorescence spectrum detection system and method based on single photon counting method

Abstract

The invention relates to a microscopic circular polarization fluorescence spectrum detection system and method based on a single photon counting method. The system is characterized in that a white light source, a detected sample, a dichroscope, a photoelastic modulator and a Glan prism are arranged in a line, and light emitted by a switchable light source system enters the dichroscope through a depolarizer; the detection switching reflecting mirror is movably arranged between the dichroscope and the photoelastic modulator, when the detection switching reflecting mirror moves in, emergent light of the dichroscope is reflected by the detection switching reflecting mirror to enter the microscopic imaging system, and when the detection switching reflecting mirror moves out, emergent light of the dichroscope enters the spectrum acquisition system through the photoelastic modulator and the Glan prism, the photoelastic modulator is connected with the photoelastic modulator controller through a line, and the photoelastic modulator controller is connected with the spectrum acquisition system through a line. According to the invention, light source and system conversion can be realized as required, and circular polarization fluorescence spectrum detection of solid samples, especially high-voltage module samples, can be realized.

Description

Technical field [0001] The present invention relates to the field of sample optical detection, and in particular, a microcarbon polarizing fluorescence spectral detection system and method based on single photon counting method. Background technique [0002] The circular polarizing fluorescence spectral detection system is the process of collecting the circularly polarized fluorescence of the radiation by the retarded excitation photointed sample molecular character spectrometry, and the detection of the circularly polarized fluorescence spectrum can assist the light-emitting characteristics of the analysis material can be assisted. [0003] The detection method of round polarizing fluorescence spectroscopy has a phase-locked phase amplifier, a mesh integral method, a single photon counting method, and the like, in these methods, the single optical sub-counting method has the advantages of high efficiency and integral region adjustable. [0004] Under the premise of using a singl...

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Problems solved by technology

[0004] On the premise of using the single photon counting method, when detecting the circularly polarized fluorescence spectrum, it is usually realized by using a xenon lamp or LED light source with a monochromator. This method can only be applied to the circularly polarized fluorescence spectrum of a liquid sample contained in a cuvette Detection, cannot be applied to solid samples, and cannot perform microscopic in-situ detection of target areas

[0005] However, for solid samples, since the distribution of solid samples cannot be as uniform as that of liquid samples, it is hoped that in-situ measurement can be achieved during repeated experiments, that is, the excitation light during multiple measurements can be irradiated on the same surface of the sample. position, which requires the excitation light source to have excellent monochromaticity and coherence, a smaller focus can be obtained when focusing, and microscopic imaging of the sample area can be performed during the measurement process, but in the prior art, there is no simultaneous A detection system that meets the above requirements

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