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Method for culturing and preparing triple nitrogen source fast-growing probiotic liquid and fleshy layer and application thereof

A probiotic liquid, meat quality technology, applied in biochemical equipment and methods, microorganism-based methods, medical raw materials derived from fungi, etc., can solve the problems of high allergenicity, slow fermentation speed, slow growth speed, etc. Not easy to be damaged by strong acid, short fermentation time, good storage and transportation effect

Inactive Publication Date: 2021-10-08
优仕康生(天津)科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The benefits of multiple probiotic liquids on gastrointestinal diseases are well known, so people call the multiple probiotic liquids represented by kombucha as "weibao", but the culture cycle of multi-component probiotic liquids represented by kombucha is longer , unobstructed at room temperature needs to be cultivated for 15-30 days, so it has been unable to meet the production needs of health care drink preparations and medicinal liquid preparations to form medical and health products that can be mass-produced
Moreover, the cultivation of multiple probiotic liquids represented by kombucha is accompanied by the growth of the biological fleshy layer (bacterium film). The bacterial film is a good carrier of the original probiotic liquid and itself has good biological properties. Slow and lack of awareness is not widely used
In addition, the probiotics formed by using black tea as a nitrogen source have side effects of tea polyphenols and theophylline, which is not conducive to long-term drinking. The nature of black tea’s nitrogen source is also an important reason why the bacterial liquid and bacterial film have not been widely used
In the prior art, there is an exploration of using aloe vera to cultivate probiotic liquid, but the problem is that aloe vera is highly irritating and highly sensitizing. From the perspective of traditional Chinese medicine, aloe vera is a serious drug and has relatively large side effects. In fact, aloe vera is known to be good The antibacterial, anti-inflammatory, and mucous membrane protective effects of Aloe vera are difficult to be utilized in the human cavity with liquid preparations; and the liquid preparations of aloe vera are extremely bitter and do not have the acceptable taste for long-term drinking as a functional drink; The fermentation speed of source-cultured probiotic liquid is slower than that of kombucha, which is not conducive to large-scale production
In the prior art, there is still the exploration of using Hericium erinaceus to cultivate probiotic liquid, but Hericium erinaceus does not have antibacterial and anti-inflammatory effects, only the effect of nourishing the stomach and neutralizing can not significantly improve the function of the probiotic liquid, and Hericium erinaceus Bacteria do not have the medicinal effect of being applied to the body cavity organs other than the gastrointestinal tract

Method used

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  • Method for culturing and preparing triple nitrogen source fast-growing probiotic liquid and fleshy layer and application thereof
  • Method for culturing and preparing triple nitrogen source fast-growing probiotic liquid and fleshy layer and application thereof
  • Method for culturing and preparing triple nitrogen source fast-growing probiotic liquid and fleshy layer and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] In this example, the preparation method and application of aloe vera, Hericium erinaceus, and berry triple nitrogen source quick probiotic liquid and its surface biological succulent layer

[0051] The culture preparation method comprises the following steps:

[0052] 1. Preparation of base liquid: Weigh 1-3 parts of functional nitrogen source, 2-4 parts of growth-promoting nitrogen-sugar dual-source berries, 2-10 parts of sugar, and 100-200 parts of water according to the mass ratio, mix and heat to Keep at 100°C for 5-30 minutes, cool to 1-45°C, and filter to remove slag to obtain the base liquid;

[0053] 2. Inoculation: transfer the base liquid prepared in step 1 to the culture container, and inoculate the probiotic bacteria into the base liquid in the culture container;

[0054] 3. Fermentation: Seal the culture container with a sterilized towel or gauze and ferment. The fermentation temperature is 30-40°C. Fermentation is longer than 24-48 hours, and the applicab...

Embodiment 2

[0069] The aloe nitrogen source of this embodiment is the active ingredient extract of aloe, including commercially available aloe gel, aloe gel freeze-dried powder, aloe gel, etc. prepared by traditional techniques. equivalent. Others are the same as embodiment 1

Embodiment 3

[0071] This example is the application of the instant probiotic liquid prepared by the cultivation and preparation method of Example 1 or 2. The original solution is made into a functional drink and directly consumed for gastrointestinal antibacterial, anti-inflammatory, and mucosal protection; the original solution is made into The enema liquid is used to detoxify the intestinal tract and soften the feces; fill the silkworm cocoon shell with absorbent cotton and set the pull wire as the carrier of the original liquid, which can be left in the vagina to adjust the pH value and flora of the vaginal microenvironment and achieve antibacterial and antibacterial effects. It is a gynecological product for the protection of inflammation and mucous membranes; the original solution is made into an injection liquid that is not used for muscles and blood vessels and is only used as a cavity (centrifuged at a speed of 6000rpm for 10 minutes to get the supernatant) and directly injected into...

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Abstract

The invention discloses a method for culturing and preparing a triple nitrogen source fast-growing probiotic liquid and a fleshy layer and application thereof. Hericium erinaceus and dark berries rich in anthocyanin intervene in probiotic liquid with aloe as a main functional nitrogen source for culture, irritation and sensitization of aloe are reduced, and the good antibacterial, anti-inflammatory and mucosa maintenance effects of aloe and the effect of probiotics are combined, which can be widely used in human body cavity. According to the invention, a second-generation probiotic liquid can be quickly generated to reach the applicable concentration, and applicable fleshy layer mycoderm with the thickness of 1-10MM can be quickly generated on the surface of the probiotic liquid within 2-7 days. According to the invention, the extremely bitter taste of the aloe preparation is overcome, conditions are created for the aloe preparation to become a functional beverage. The fast-growing fleshy layer mycoderm disclosed by the invention can be applied as a native carrier of probiotic liquid and can be applied to multiple fields according to the biological characteristics of the fast-growing fleshy layer mycoderm. In addition, the invention provides a fresh-keeping liquid for fresh-keeping storage of the fresh aloe.

Description

technical field [0001] The present invention relates to a method for cultivating and preparing a triple nitrogen source rapid probiotic liquid and a succulent layer and its application, and relates to a method for cultivating and preparing a triple nitrogen source rapid probiotic liquid and its surface biological succulent layer and its application, belonging to Functional food and beverage and biomedicine fields. Background technique [0002] The benefits of multiple probiotic liquids on gastrointestinal diseases are well known, so people call the multiple probiotic liquids represented by kombucha as "weibao", but the culture cycle of multi-component probiotic liquids represented by kombucha is longer , Unobstructed at room temperature needs to be cultivated for 15-30 days, so it has been unable to meet the production needs of health care drink preparations and medicinal liquid preparations to form medical and health care products that can be mass-produced. Moreover, the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P1/02A61K36/896A61K36/886A61P29/00A61P31/04A61P15/00A61P15/02A61P1/04A61P1/00A61P9/14A61K8/9794A61K8/49A61K8/9728A61K8/99A61K8/02A61K8/98A61Q19/00C12R1/02C12R1/645C12R1/01C12R1/46C12R1/225C12R1/23A61K35/744A61K35/747A61K35/745A61K35/741A61K31/352
CPCC12N1/20C12P1/04C12P1/02A61K36/896A61K36/886A61K36/07A61K36/064A61K35/744A61K35/747A61K35/745A61K35/741A61K31/352A61P29/00A61P31/04A61P15/00A61P15/02A61P1/04A61P1/00A61P9/14A61K8/9794A61K8/498A61K8/9728A61K8/99A61K8/0212A61K8/986A61Q19/00A61K2236/19A61K2800/85A61K2300/00
Inventor 刘金良
Owner 优仕康生(天津)科技发展有限公司
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