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Drug-resistant breast cancer treatment drug and screening method thereof

A screening method, breast cancer technology, applied in the field of medicine, can solve problems such as blocking the effectiveness of breast cancer treatment, achieve the effect of strengthening cancer treatment effect, increasing the number, and reducing the level of glycolysis

Inactive Publication Date: 2021-09-28
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent decades, breast cancer treatment technology has developed greatly, and anti-breast cancer first-line treatment drugs emerge in endlessly, but the occurrence of drug resistance hinders the effectiveness of breast cancer treatment to a large extent.

Method used

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  • Drug-resistant breast cancer treatment drug and screening method thereof
  • Drug-resistant breast cancer treatment drug and screening method thereof
  • Drug-resistant breast cancer treatment drug and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1. Drug screening method and result

[0056] 1.1 Confirm the resistance of breast cancer drug-resistant cell lines MCF-7-TR and T-47D-TR to known breast cancer drugs

[0057] Firstly, the resistance of MCF-7-TR and T-47D-TR to different breast cancer drugs was analyzed. After the two drug-resistant cell lines were treated with different concentrations of breast cancer drugs, the drug resistance index was evaluated by MTT experiment.

[0058] The cells were seeded into 96-well plates (2000 cells / well), and after the cells adhered to the wall overnight, they were treated with different concentrations of drugs for 24h, 48h or 72h, respectively. Then add 20 μL of MTT solution (5 mg / mL) to each well, place in a 37°C incubator and continue to incubate for 4 hours, remove the old medium in the well, add DMSO (100 μL / well) and mix well on a horizontal shaker. The OD value was detected at 570 nm using a microplate reader. The results were expressed as the inhibition...

Embodiment 2

[0087] Example 2 Drugs to be screened inhibit proliferation of breast cancer drug-resistant cells

[0088] According to the experimental results of each step in Example 1, only baicalein can meet the expectations of the comprehensive evaluation. The principle of the comprehensive evaluation is that the drug to be screened needs to reach the following three test results at the same time: (1) significantly increase the reference drug-induced resistance (2) It can down-regulate the lactic acid level of drug-resistant cells and the expression levels of GLUT-1, PDK-1, LDHA and other proteins; (3) It can at least increase the mitochondrial function of cells, increase Mitochondrial number, increased mitochondrial / intracellular ROS, and downregulated changes in mitochondrial membrane potential had more than two effects. The purpose of this experiment is to further test the effect of the drug to be screened on the reference drug to inhibit the proliferation of drug-resistant cells, in ...

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Abstract

The invention relates to a drug-resistant breast cancer drug screening method and an obtained effective treatment drug. The drug-resistant breast cancer drug screening method comprises the following steps that a drug-resistant breast cancer cell strain is selected, the resistance of the cell strain to an anti-breast cancer drug is confirmed, and a reference drug is selected; and the influence effect of a to-be-screened drug on cell apoptosis inhibition of a reference drug is detected, the influence on the glycolysis level of drug-resistant cells and the influence on mitochondria of the drug-resistant cells is detected, and the screened baicalein with excellent comprehensive evaluation is an effective anti-breast cancer drug sensitizer. The drug-resistant breast cancer drug screening method is especially suitable for obtaining a matched sensitizing drug aiming at a specific drug-resistant drug, so that precise medical treatment aiming at a specific drug-resistant breast cancer patient is facilitated.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a screening method for drug-resistant breast cancer drugs and the obtained effective therapeutic drugs. Background technique [0002] Breast cancer is the first malignant tumor that seriously threatens the health of women all over the world. In recent decades, breast cancer treatment technology has developed greatly, and anti-breast cancer first-line treatment drugs emerge in an endless stream, but the occurrence of drug resistance hinders the effectiveness of breast cancer treatment to a large extent. The generation of tumor drug resistance can be divided into primary drug resistance and acquired drug resistance. According to the drug resistance characteristics of tumor cells, the acquired drug resistance can be divided into primary drug resistance (PDR) and multidrug resistance. Drugs (multidrug resistance, MDR) two categories. PDR refers to cross-resistance to inducing d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02A61K31/352A61K31/138A61P15/14A61P35/00
CPCG01N33/5011A61K31/352A61K31/138A61P15/14A61P35/00C12N2503/02G01N2500/10A61K2300/00
Inventor 陈妍沈祥春张璟钰胡晓霞张敏琴陈宗跃酆啸
Owner GUIZHOU MEDICAL UNIV
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