Method for screening and constructing primary hepatocyte klotho gene transduction stem cells
A construction method and stem cell technology, applied in the field of primary liver cell klotho gene transduction stem cell screening and construction, can solve the problems of difficult to achieve ideal amplification effect, high cost, and difficult application, so as to improve survival ability and success rate , improve the effect of self-renewal
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[0013] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0014] A method for screening and constructing primary hepatocyte klotho gene-transduced stem cells, a method for screening and constructing primary hepatocyte klotho gene-transduced stem cells, the specific steps of the method for transducing stem cells are:
[0015] S1: Use the whole genome DNA of the human being to be tested containing the klotho gene as a template, and then amplify the polymorphic DNA sequence of the exon 4 region of the klotho gene by PCR, and the DNA seq...
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