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Saccharopolysporaspinosa with high yield of spinosad

A technology of Saccharopolyspora spinosa and spinosyn, which is applied in the field of microorganisms and can solve the problems of low yield of spinosyn and the like

Active Publication Date: 2021-09-28
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of low yield of spinosad produced by fermentation of existing Saccharopolyspora spinosyn, and to provide a Saccharopolyspora spinosa with high yield of spinosad

Method used

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  • Saccharopolysporaspinosa with high yield of spinosad
  • Saccharopolysporaspinosa with high yield of spinosad
  • Saccharopolysporaspinosa with high yield of spinosad

Examples

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Embodiment 1

[0017] 1. Isolation of spinosad strains

[0018] In July 2017, soil samples were collected from multiple regions, and the actinomycetes were separated. The separation method is: fill the soil with sterile water to 0.1g / mL, suspend the soil with sterile water, and add 10 glasses Beads were then serially diluted 10-fold, and 10 pieces of separation medium were coated for each concentration. The basic formula of the separation medium is: add 5g of glucose, 3g of yeast extract, and 10g of enzymatic casein (N-Z amine type A) to 1L of distilled water, mix and dissolve fully and adjust the pH to 7.0 with NaOH; 200mL aliquots, add 4g of agar to each bottle; sterilize at 115°C for 30min. After the sterilization is completed, cool to about 65°C, add nalidixic acid to a final concentration of 25 μg / mL to inhibit the growth of Gram-negative bacteria, and nystatin to a final concentration of 50 μg / mL to inhibit fungal growth. Among the colonies that can finally grow, the Streptomyces sam...

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Abstract

The invention discloses Saccharopolysporaspinosa with high-yield of spinosad, and belongs to the technical field of microorganisms. The Saccharopolysporaspinosa with high-yield of the spinosadis SaccharopolysporaspinosaWHU1107 with the preservation number of CCTCC NO: M 2021307, the strain is obtained after mutagenesis, the yield of the spinosad is up to 4 g / L or above after fermentation culture is conducted on the strain in a culture flask containing 25 mL of a fermentation culture medium, the yield is far higher than that of existing reported strains, and the Saccharopolysporaspinosa can be used for producing the spinosad. The invention provides a new material for industrial production of the spinosad.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Saccharopolyspora spinosa with high spinosad production. Background technique [0002] Spinosyns (spinosyns) are secondary metabolites obtained after aerobic fermentation of Saccharopolyspora spinosa, and are a new type of green broad-spectrum biopesticides belonging to the Ester antibiotics. The unique insecticidal mechanism of spinosyn shows the safety of biological pesticides and the rapidity of chemical pesticides, and due to the easy degradation of spinosyns, it is harmless to the environment, crops and mammals, and has no carcinogenicity, teratogenicity, or mutagenicity or neurotoxicity, so it has won the "Presidential Green Chemicals Challenge Award" in the United States three times. Currently, spinosyns are still produced by aerobic fermentation by S. spinosa. Although there are many patent reports to increase the yield of spinosyn by improving the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/62C12R1/01
CPCC12N1/20C12P19/62
Inventor 刘天罡刘然邓子新
Owner WUHAN UNIV
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