Separation and purification method of salmonella pullorum specific lytic bacteriophage

Abstract

The invention belongs to the technical field of bioengineering, and discloses a separation and purification method of salmonella pullorum specific lytic bacteriophage, which comprises the following steps of: separating salmonella pullorum bacteriophage; identifying the bacteriophage by adopting a spot method and a double-layer agar plate method; purifying the bacteriophage by adopting the double-layer agar plate method; and determining the titer of the bacteriophage: counting plaques by adopting the double-layer agar plate method, and calculating the titer of the bacteriophage according to a relational expression between the titer of the bacteriophage and the number of the plaques. According to the invention, clinical isolates of salmonella pullorum are taken as host bacteria; a strong-splitting bacteriophage YSP2 is separated and screened from sewage; the bacteriophage belongs to caudal viridae, is stable in biological characteristic, specifically splits salmonella, and has a better treatment effect on pullorum disease; and the optimal treatment effect can be achieved when the bacillus subtilis and the host bacteria enter a human body at the same time.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for separating and purifying specific lytic phages of Salmonella pullorum. Background technique [0002] At present: Pullorum pullorum is a common infectious disease caused by Salmonella pullorum pullorum. On the one hand, the disease has caused serious harm and economic losses to the global breeding industry; The emergence of drug-resistant bacteria and antibiotic residues in poultry products have seriously endangered human health. As a natural enemy of bacteria, phages can not only overcome the problems of strain resistance and drug residues, but also can be easily obtained in nature, and are gradually being researched and utilized as a substitute for antibiotics. [0003] Salmonella pullorum is widely spread in developing countries, causing serious economic losses to the poultry industry. Antibiotic treatment of pullorum still exists in many count...

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Problems solved by technology

[0005] (1) There is no effective preparation for removing Salmonella pullorum in chickens and in the environment

[0006] (2) Antibiotics can play a certain role in the prevention and treatment of Salmonella pullorum, but the problem of drug resistance affects the effect of antibiotics

[0007] The difficulty of solving the above problems and defects is: Salmonella pullorum can be transmitted horizontally and vertically, and has intracellular parasitic characteristics, and the resistance in the environment is strong, so it leads to the removal and purification of Salmonella pullorum in chickens and in the feeding environment more difficult

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